LOCUS       sequence-368166-       12253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA 
            and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Brown JT, Haraczy AJ, Wilhelm CM, Belanger KD
  TITLE     Characterization of nuclear pore complex targeting domains in Pom152
            in Saccharomyces cerevisiae.
  JOURNAL   Biol Open. 2021 Oct 15;10(10). pii: 272274. doi: 10.1242/bio.057661.
            Epub 2021 Oct 20.
  PUBMED    34557894
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2021-10-15"; volume: "10(10). pii: 272274. doi"; 
            pages: " 10.1242/bio.057661. Epub 2021 Oct 20"
COMMENT     Sequence Label: KBB521
FEATURES             Location/Qualifiers
     source          1..12253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(578..646,647..1438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(796..815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1609..2197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2098..2117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2351..2368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2485..2506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2521..2526,2527..2544,2545..2551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2559..2575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2564..2586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2583..2599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2583..2599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2620..2638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2666..2682
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2667..2683
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4061..4087
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4103..4129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4145..4171
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6182..6892
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6209..6237)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6832..6852
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(8964..8980)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8964..8980)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9012..9031)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9013..9031)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9038..9055)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9038..9054)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9047..9069)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9195..9650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9282..9301)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9492..9513
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9951..10358
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10359..11453
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11947..11966)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12066..12088
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12126..12144)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(12186..12253,1..436)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa
       61 gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt
      121 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
      181 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
      241 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
      301 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
      361 tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat
      421 ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac
      481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      541 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      601 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      661 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      721 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      781 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      841 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      901 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      961 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1021 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1081 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1141 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1201 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1261 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1321 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1381 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1441 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1501 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1561 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1621 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1681 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1741 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1801 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1861 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1921 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1981 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2041 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2101 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2161 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2221 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2281 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2341 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2401 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2461 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2521 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2581 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
     2641 acaaaagctg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
     2701 ttcctgcagc ccgggggatc cacagttgac aacgatctca agattggttg ggatttaaat
     2761 cctatagcag tgcatcagaa gaagcaaaaa ggccagtgga cagtcattaa gtttatcacc
     2821 agaaagggct tccagaccat cacaggtgaa gagaaagaaa aaaaataatg cgctggtatt
     2881 atagaaatag agtttcatta tataagaaca tataggaaga atacattatt cctatctagg
     2941 gcatcaaata attgcaacca cttaatgtta acttccttca taattcaaaa cattatgttc
     3001 tatagcacta tgtattttat gtcattatat acgtagtcaa taactttata cgtttttttt
     3061 attgaacaaa agtaagaatt gcttaagaca taataagagg aaactcatcg aaccaattca
     3121 aaacggatta tagatttatc ataccagata cgtttatcag gaggcttgta taatggagca
     3181 cagatataac gtgtttaatg atactccacg agggaaccat tggatgggca gttctgtgtc
     3241 aggatcacca cgtccatctt atagcagtcg tcctaatgta aacacaacac gacgcttcca
     3301 atatagtgac gatgagcccg ctgagaaaat ccgaccttta cgctcgagga gctttaaaag
     3361 tactgaaagt aacataagcg atgaaaaatc aaggatatct gaacgtgaca gcaaagaccg
     3421 ctacattaat ggtgataaaa aggtagacat ttattcactg cctctaatat caaccgatgt
     3481 tttagaaatt tccaaacaaa ggacatttgc ggtgatattg tttttaataa ttcaatgtta
     3541 taaaatatat gacctggtaa tacttaaatc ggggttaccg ctttcgggtt tactgtttaa
     3601 gaactatcga tttaatttca tatccaagta tttcattata gattccttct tcctgtatgt
     3661 tttaccatcc tttaacattc ctaggttgac ttttaagcca tgggttgttt atctccagat
     3721 tttagctatg ctactattga acattttcat atcaagcgat cacgagttcg ttttgatttc
     3781 attaattatg accacatgga gaaaacttta tacgaaagaa ttaagtgtaa caggttcagc
     3841 cattaaccat catcgtattt ttgattcttc cgctcatttt aagggtgctc taacaatcaa
     3901 gatcctacct gaaaacacag ctatgtttaa tcctcttcat gaatcatatt gtttgcccat
     3961 ggataccaat ctttttaaaa ttaactcaat tgacgtaccc atcagaataa actcaactga
     4021 agagattgaa tatatcgagt tggaatatag agatcttggt tacccatacg acgtcccaga
     4081 ttacgctagc ggtgatcttg gttacccata cgacgtccca gattacgcta gcggtgatct
     4141 tggttaccca tacgacgtcc cagattacgc tagcggtgat ctttacacaa attcggtaga
     4201 gttacggtct ttgagtaaga aagacttcaa aatcatcgat aaccccaaat cctttttaaa
     4261 aaaagatcag tcagttctaa agtcacattc aaacgatttt gaagaaggca gtacaattcg
     4321 ttatttggct gtaactcttc aagatattgg cttttatcaa atcaaaaaaa ttgttgattc
     4381 aaaaaaactg aatttaaaga tccatcagtc acacttggtt gtaccctact gtccaattgc
     4441 atctattact ggaactggaa gcaatgatag atgtattggc gattcagata atgtttcttt
     4501 tgaaattcaa ggtgtgcccc caatgaaatt agcatactct aagatcgtta atggtcaaac
     4561 tttttcatat gtagactcca gcttgcaacc agaatatttt gagtctcctt tgcagtcaag
     4621 caaatccaaa caaagtttta ctcaaggaga actaaacgat ttgaaatggg gaagaaacca
     4681 acctgttaat attaatttag actcttccat tacccaggac ggcaaatttg catataaaat
     4741 tgataaaata actgatggtc tgggaaatgt cgtggatttt acatctctcc ctgaagaatt
     4801 aaaaaagcgc tatgatctat cttataattt caatgttcat gaggttccac gtgcagcgct
     4861 agaagaaaga tttgatccaa agtcacccac aaaaaggtct attgccatcg tgtttgaaga
     4921 aatcaaaaac tggatttcag acattccata tgttattagc ctatcctata ctgatgcaca
     4981 agacaagtcg aaaaagataa tgaatgttac tacagattct ttgaccaaag tgttgcaagc
     5041 ggatcttccg gggtcatata acttagaata tattgaatct aagttttgtc caggtgaaat
     5101 tgtgggtaaa tcaaacgttc ttgtaactat gccagttgca cctactatgg aagttaaatc
     5161 atttccaata ttggaccagt gtgtgggaca ggttggtcta aactttgaac tatcttttac
     5221 tggggctcca ccatactatt ataacactaa aatctataag cttgaaaacg gtgaaagaaa
     5281 gttatatgat gcaaagagat acacctctga aggtacgaga aatcgcttca gctatagtcc
     5341 acctaaagaa ggtaactatg aaatcgtgtt tgatactgtt tctaataaat tattcactga
     5401 accaatcaaa ttggaacctg taaaagaata tactttcaag acatcaatga gggtgaaacc
     5461 aagcgcatca ctaaaattac accatgattt gaaactctgt ttgggtgacc acagcagtgt
     5521 cccagtagca ctaaaaggcc aaggcccttt tacgttaaca tatgatatca ttgaaacttt
     5581 ttctagcaag aggaaaactt ttgaaattaa agaaataaaa accaacgaat atgtcattaa
     5641 aacaccagtc tttactactg gtggtgatta tattctatct ttggtttcta tcaaggattc
     5701 taccggctgt gtagttggac tcagccaacc agatgctaaa atacaggtga ggagagatat
     5761 tccatctgct gcattcaatt tttttgaacc tatcaaagaa gctaaaatta agcacggttc
     5821 tgtcactgaa attccgctca aattaagtgg agaggggcca ttcaccgtta agtttaaaca
     5881 catggattac gatggaaaca ttgtgaaaga atttgaaaac aaatttcaga atagttataa
     5941 gcccgctttg aaagtaagta aagaagggct ttaccaattg gtcgatattc gtgactcaag
     6001 ttgccaaggt aatgtgattt accggaacag tctgtataag gtatctttct tggagaagcc
     6061 aaaatttgca attcaagaca atcatcacat tacaaaagta acggaaaatt tattctctaa
     6121 agaagaagtc tgtcaaggga tggaaggtac ggttgatttg gctctatttg gttctccagc
     6181 tagcaaagga gaagaactct tcactggagt tgtcccaatt cttgttgaat tagatggtga
     6241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     6301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     6361 cactactttc acttatggtg ttcagtgctt ttcaagatac ccggatcata tgaaacggca
     6421 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     6481 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     6541 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg ggcacaaatt
     6601 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     6661 caaagctaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     6721 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     6781 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     6841 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaggatccgg
     6901 ctgctaacaa agcccgaaag gacggtctat agaggtgtgt taaaagcaga cttttgaaca
     6961 tccacagaaa ttggctcaat gacctatccg aaaagaatac ctataaatgc gtggtctgaa
     7021 gttcataggg tggcaaggcc actaattatt actttcgacg catataatac actatacgcc
     7081 acaaaacttc ccgtcatgga acaatactgc attgttgggc gtaaatatgg tatcaaggcg
     7141 aatccttcga ctttaacaaa caattttcca cacgttttta aaaaactgaa ggaagactat
     7201 ccccaatacg gaaaatattc tggtatcaag cccgaacaat ggtggtccat cttgattagg
     7261 aacgtcttcg caccaaacga aattcccgat gagatgataa atgaaatatt aatgcggttt
     7321 gagggcttcg attcgtattt tgtttatcca gatttgatca aatttttaaa ggatttgaag
     7381 tcaagacatc ctgatgttat cttaggaata gtaagcaata ctgatccaat tttttacaaa
     7441 ttattaaaga atatcggatt atttgagaca ttttccggtc acatttattt atcatatgag
     7501 cttaatctcg ccaaacctga tagagcaata tttcaatatg cgcttgacga tattatatct
     7561 aaacaacctc atttactcga aaagtacacc agggaggaaa ttctacagca ttgctttcac
     7621 atcggagatg aattaaagaa cgatctcgaa ggggcagaag ctgccggttg gactggtata
     7681 ctgttagata gaaatgataa atacgggttt ctttcaaact cgattagtaa accaatgcgg
     7741 gacgaataca agctgtcaat tgataaaata gataataatt caataaacac ttgggaagcc
     7801 aatactaagc aaactgacac gttacagttg agtgaaagaa agtacgttgt ttcgaatctt
     7861 gaggttttag aggaactctt tccctgagcg cctaacgtta cagtaacaaa tatgtatgat
     7921 agatattatt catatccgcc agtaatattt actatatctt tgaatgggtt tgtttattaa
     7981 taatctacta tgtgtaaata tatgatccaa gtattataca gtttagacac tgattgtttt
     8041 ccagacgttc aaaccgtcag taccggtact tactaaacaa cctgggattt gcttatgcca
     8101 tttgacatct ttaacttcct tttgccagtg aacaaacaat aattgtggtg gaatttcttg
     8161 tagctctttt gtttcggcgg cctgttgttt aatttcctca tcgtcagctt ctacagacaa
     8221 atcccacaaa gtcacagtat tatcttctga gcctaccgca acgatagact catctaatgg
     8281 gttgaatgca atggaagtaa tggctccctt atggaagtca tattgagcaa ccggttggac
     8341 ggcgtcagca ttacttggcg taaactgtct taaatcccat actccccatg taccgttatc
     8401 gtcaccgctt gctagcaagt aaccaatttt atcactccaa cttatgacat ttacgtcagt
     8461 attagaagct ttaacagaga tagcaggttt atgttttttt gatcttgtgt cccaaatcct
     8521 tatatatcca tcacatcctg cggttgcaaa aacggtggat tcagtgcgag accactggat
     8581 atcttctatg gatttattgt ttgaaacagt aaatggttgt ttatcagtca cccatctcga
     8641 tgtgtgcctt tgggtaaaat atatttgtcc tgagcaatca cctgatagta acgcaccagt
     8701 cttgatcaat ggtgaccaat ccaacccgta gccttcaaca ttcccatgat tttttacagt
     8761 gtgaatagga cgcttagcag acttcggaat ctgataacct ggtgtggaaa aagctttgct
     8821 ttgtggagct agattgtata tataaacatc accattttcg ctcattgtag cggttaacac
     8881 ctcttgatta gaaatggcaa aaggagaaac ctttaatcta ttggttgtat ctctcaatgg
     8941 tatattttca ttctcaatga ctggatccac tagttctaga gcggccgcca ccgcggtgga
     9001 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     9061 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     9121 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     9181 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     9241 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     9301 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     9361 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     9421 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9481 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9541 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9601 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg
     9661 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatcgaccgg tcgaggagaa
     9721 cttctagtat atctacatac ctaatattat tgccttatta aaaatggaat cccaacaatt
     9781 acatcaaaat ccacattctc ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt
     9841 tcaaaaacgt tatatttata ggataattat actctatttc tcaacaagta attggttgtt
     9901 tggccgagcg gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt aactgtggga
     9961 atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct
    10021 caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat
    10081 tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag
    10141 aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga agcgttcatg
    10201 actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt
    10261 ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag
    10321 caatatatat atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg
    10381 tcgttttgcc aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta
    10441 aagctatttc tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg
    10501 gtgctgctat cgatgctaca ggtgttccac ttccagatga ggcgctggaa gcctccaaga
    10561 aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc ggtagtgtta
    10621 gacctgaaca aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac
    10681 catgtaactt tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta
    10741 aaggtactga cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa
    10801 aggaagacga tggtgatggt gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc
    10861 aaagaatcac aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt
    10921 ggtccttgga taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg
    10981 aaaccatcaa gaacgaattc cctacattga aggttcaaca tcaattgatt gattctgccg
    11041 ccatgatcct agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt
    11101 ttggtgatat catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat
    11161 ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc
    11221 acggttctgc tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg
    11281 ctgcaatgat gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg
    11341 cagttaaaaa ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta
    11401 ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt
    11461 ctcttttttt atgatatttg tacataaact ttataaatga aattcataat agaaacgaca
    11521 cgaaattaca aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata
    11581 catttatcaa gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact
    11641 aatggctcaa cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga
    11701 gggcaccaca caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat
    11761 atattggagg attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc
    11821 tgaccatttg atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag
    11881 ttccctcaag aattttactc tgtcagaaac ggccttaacg acgtagtcga tatggtgcac
    11941 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
    12001 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
    12061 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
    12121 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
    12181 ggacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt
    12241 gggaatttac tct
//
