LOCUS       sequence-368168-        9179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Swi6L-GFP with nuclear export signal mutated to alanines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Belanger KD, Yewdell WT, Barber MF, Russo AN, Pettit MA, Damuth EK, 
            Hussain N, Geier SJ, Belanger KG
  TITLE     Exportin Crm1 is important for Swi6 nuclear shuttling and MBF 
            transcription activation in Saccharomyces cerevisiae.
  JOURNAL   BMC Mol Cell Biol. 2022 Feb 21;23(1):10. doi: 
            10.1186/s12860-022-00409-6.
  PUBMED    35189816
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Cell Biol."; date: "2022-02-21"; volume: "23(1):10. doi"; pages: " 
            10.1186/s12860-022-00409-6"
COMMENT     Sequence Label: KBB418
FEATURES             Location/Qualifiers
     source          1..9179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3173..3889
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3206..3234)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3829..3849
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(4237..4255)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4276..4292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4276..4292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4289..4311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4300..4316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4324..4330,4331..4348,4349..4354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4369..4390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4507..4524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4678..5266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4758..4777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5437..6228,6229..6297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6060..6079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6298..6402)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6439..6942
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     6983..7001
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7039..7061)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7161..7180
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7201..7481
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7482..8156
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8259..8714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8396..8417)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8608..8627
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8840..8862
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8854..8871
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8855..8871
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8878..8897
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8878..8896
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     8929..8945
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8929..8945
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gccaatttac tcaaataaat tttgcaatac ttaattcgtt tttgtaatga atttgtactc
       61 gtttcactta tattttcctt ccacaggtgt aaaatgcatg aaaataaatc aatacaacac
      121 caaagtgatc tttttaacat aacatcccag ggaccatctt ttagctgttt cagtttctcg
      181 ttatacaacg acattgtaaa atttagcata taaattttgg ctttttcttg atcatgtatt
      241 agcgtgttca tatcgtgtaa gcttttgtca ctgtcttctg cttcactctc gccgccaatc
      301 tccttcttaa acgcttcaat agtatcaaga agttctgtcg ctagtgcggt catctcttgc
      361 gaccgatctt cttcactcag tatcaattcc accgcatata actgaagata gtatccgatg
      421 atccctaacc caactttgtc aaaatccttc gctgtagcga caacccttgc agcgttacta
      481 gccatagaat ttatttaaaa tgtggacaat tagtttctaa aaattgaatc aaataaaata
      541 agaaagctta ttgttccttt ttttgtcttc acctggaccg tttggataag atcattcacc
      601 aaatcttagt tttttttttt agtttccttc tccaacgatt tcacaataat aaaaaaaaac
      661 aacaaaaaca cgattgagaa acatcaatat gaactaagca atagctaaaa aaaaaaagaa
      721 taataaaggg gaacacagta taattctcga gaggatggcg ttggaagaag tggtacgata
      781 cttaggacct cacaatgaga tccctttgac actcactaga gactcggaga ctggccattt
      841 cctcctgaaa cattttctgc ccattttgca gcaatatcat gacacgggga atattaacga
      901 gaccaacccc gatagtttcc ccactgatga ggaaagaaat aaattactgg cacattatgg
      961 aattgctgta aatacagacg accgaggtga gttatggata gagctggaaa aatgtttaca
     1021 attattaaac atgctaaatt tattcggttt gttccaggat gcattcgaat ttgaggagcc
     1081 tgaaacagat caggatgaag aagatcccag ccattcgaaa ctaccagaaa acaagaccaa
     1141 aagtgaaaat tctaaggata atatcagctc aaagaggatt aataatttac aagatatgag
     1201 cctggattct gatgcacaca gagaattagg ctctccttta aaaaaactaa aaatagatac
     1261 ctctgtaata gatgctgaga gtgactccac tccgaatact gccagaggca agcctaacga
     1321 tgatattaat aagggcccta gcggcgacaa tgaaaataat ggcactgatg acaatgacag
     1381 aaccgctgga cctatcataa cattcactca tgacctaact tctgactttt taagcagtcc
     1441 actgaaaatc atgaaagcac taccttctcc agttgtaaat gataatgaac agaagatgaa
     1501 agcagaggca ttcgcacaac ggttgttatt tccagaaatt caagaaatgc ctacatccct
     1561 taataatgac agcagtaata gaaattcaga aggggggagc tcaaaccaac aacaacagca
     1621 cgtatcattt gatagccttt tgcaagaggt aaacgacgct tttcctaata ctcaattaaa
     1681 tcttaatatt cctgtagatg agcatggaaa cacaccatta cattggctga cttcaatagc
     1741 aaacctggaa ttagtgaaac acctggttaa gcatggttca aacagattat atggtgataa
     1801 tatgggggag tcatgcctag tgaaagctgt caaatcagta aataattacg actctggtac
     1861 ttttgaggca cttctagatt atttatatcc atgtttaatt ttggaagact caatgaatag
     1921 aacaattttg caccatatca ttattacgtc tggtatgact ggctgttcag cagctgcgaa
     1981 atattattta gatattttaa tgggatggat tgtcaagaaa caaaatagac ccattcaaag
     2041 tggtactaac gaaaaagaaa gcaaaccgaa tgacaaaaat ggggaaagaa aggactctat
     2101 actagaaaat ttggacctga aatggataat agcaaacatg ctcaatgcgc aggactctaa
     2161 tggcgatact tgcttgaaca ttgcagcaag attgggaaac atttcaatag tagacgcttt
     2221 attagattac ggtgctgacc catttattgc aaacaaatca gggttgagac ccgtggattt
     2281 tggggcaggt acttcaaaat tacaaaatac gaacggcggt gacgaaaact cgaagatggt
     2341 ctctaagggt gattacgacg gtcaaaaaaa tggaaaggcc aaaaaaataa ggtctcaact
     2401 gttgaaaaac ccacctgaaa caacttcgtt aattaatgat gtccaaaatt tactgaattc
     2461 aatctcgaaa gattatgaga atgaaacagt gcaatataat gagaaattag aaaaactaca
     2521 caaggaattg aacgaacagc gagaagaatt agctaattct agagaacaac tagcaaatgt
     2581 gaagcaattg aaagatgaat actcactaat gcaagagcaa ttgaccaatt taaaagcagg
     2641 aattgaagaa gaagaggaaa gttttaggga agagagcaaa aagctaggaa taattgcaga
     2701 tgaaagttca ggtattgatt gggactctag tgaatatgat gcagatgaac ccttcaaagt
     2761 agagttcctt tcagatttct tggaggataa attacaaaag aattatgaag gcgatatttc
     2821 caaactatta gaagcggagt cgaaagagca gataatggaa cagatacgaa atcaacttcc
     2881 cgcagaaaaa atccaatcga tgcttccacc aacggtctta ttgaaggcaa gaataaatgc
     2941 atacaagcgg aatgataaac acctaaccaa tgtattggat acaatcagca ccaaacaatc
     3001 agaattagag aataaattta gaagagtgtt atctttatgt ttgaaaatcg acgaaaataa
     3061 agttgacaat atgcttgatg gtttgttaca ggccatatct tctgaagacc cacaagacat
     3121 tgacactgac gaaatgcaag attttttaaa aaagcatgct tcaggtcgag ctatggctag
     3181 caaaggagaa gaactcttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     3241 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     3301 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     3361 tactttcact tatggtgttc agtgcttttc aagatacccg gatcatatga aacggcatga
     3421 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     3481 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     3541 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttgggc acaaattgga
     3601 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     3661 agctaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     3721 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     3781 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     3841 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaag gatccggctg
     3901 ctaacaaagc ccgaaaggaa gcttcggtat acttattttt actccccctt ctgtgttttc
     3961 ttcttttttt tttttttttt tttttggaat ttactgttgt ttctttcgtg gctgtttctt
     4021 aatcttatac gtacctttat ctatttccgt acagttgtaa tctatcttct ttagatcttg
     4081 ccatttttta tgcggtatat cccaatggca ttttttagct acgagactgg ccacgtcatc
     4141 acagaatccc aacaggttta agtcaaactc tgcatgtgta accatatccc tattgatcaa
     4201 aatctgcgga acatgtgaag gtaccagctt ttgttccctt tagtgagggt taatttcgag
     4261 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4321 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4381 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4441 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4501 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4561 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4621 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5101 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5221 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5281 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5341 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5401 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5461 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5521 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5581 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5641 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5701 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5761 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5821 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5881 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5941 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6001 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6061 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6121 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6181 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6241 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6301 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6361 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6421 gccacctggg tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata
     6481 taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt
     6541 tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct
     6601 cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac
     6661 acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc
     6721 tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac
     6781 ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt tactttctaa
     6841 aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc
     6901 attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtctaag aaaccattat
     6961 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     7021 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     7081 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     7141 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatagatc
     7201 acgacattac tatatatata atataggaag catttaatag aacagcatcg taatatatgt
     7261 gtactttgca gttatgacgc cagatggcag tagtggaaga tattctttat tgaaaaatag
     7321 cttgtcacct tacgtacaat cttgatccgg agcttttctt tttttgccga ttaagaatta
     7381 attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga ctattgagca
     7441 cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt attaatttca
     7501 caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag gccgcagaat
     7561 gtgctctaga ttccgatgct gacttgctgg gtattatatg tgtgcccaat agaaagagaa
     7621 caattgaccc ggttattgca aggaaaattt caagtcttgt aaaagcatat aaaaatagtt
     7681 caggcactcc gaaatacttg gttggcgtgt ttcgtaatca acctaaggag gatgttttgg
     7741 ctctggtcaa tgattacggc attgatatcg tccaactgca tggagatgag tcgtggcaag
     7801 aataccaaga gttcctcggt ttgccagtta ttaaaagact cgtatttcca aaagactgca
     7861 acatactact cagtgcagct tcacagaaac ctcattcgtt tattcccttg tttgattcag
     7921 aagcaggtgg gacaggtgaa cttttggatt ggaactcgat ttctgactgg gttggaaggc
     7981 aagagagccc cgaaagctta cattttatgt tagctggtgg actgacgcca gaaaatgttg
     8041 gtgatgcgct tagattaaat ggcgttattg gtgttgatgt aagcggaggt gtggagacaa
     8101 atggtgtaaa agactctaac aaaatagcaa atttcgtcaa aaatgctaag aaataggtta
     8161 ttactgagta gtatttattt aagtattgtt tgtgcacttg ccgatctatg cggtgtgaaa
     8221 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     8281 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     8341 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     8401 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     8461 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     8521 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     8581 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     8641 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     8701 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8761 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8821 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     8881 tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt
     8941 ggatcccccg ggctgcagcg tttacgctat cggcatcatc aataaattca ggtacctcat
     9001 ccacaggctc gtatgatgtt gtttggtctt ttggggctag gccttcgact ttatcattat
     9061 tatcgttttc caaatcgctt gtctggtgat caacatcttc atccttaatc tcctcgctat
     9121 catccgcaaa atctgcataa tctaacgttt tctcgtcgct ggaaccaatt tcacccttt
//
