LOCUS       sequence-36818-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kasler HG, Victoria J, Duramad O, Winoto A
  TITLE     ERK5 is a novel type of mitogen-activated protein kinase containing 
            a transcriptional activation domain.
  JOURNAL   Mol Cell Biol. 2000 Nov;20(22):8382-9.
  PUBMED    11046135
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2000-11"; volume: "20(22)"; pages: "8382-9"
COMMENT     Sequence Label: pCI-HA-ERK5-FL
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          105..237
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     220..239
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        282..300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             316..342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             322..348
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 aaactatgcg gtgttatcac agnttaattg ctaacgcagt cagtgcttct gacacaacag
       61 tctcgaactt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta
      121 caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc
      181 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg
      241 tccactccca gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg
      301 ctagcgggta tggcttaccc atacgatgtt ccagattacg ctagtttgcc caccatggcc
      361 gaaccgctga aggaggagga cggcgaagat ggctctgggg agccccctgg gagggtgaag
      421 gcagaacccg ttcacaccgc tgcctctgtg gtggccaaga acctggccct gctcaaagcc
      481 cgctccttcg acgtgacctt tgacgtgggg gacgagtacg agatcatcga gaccataggc
      541 aatggggcct acggggtggt gtcttcggcg cgccgccgcc tcacgggcca gcaggtggcc
      601 atcaagaaga tacctaatgc ttttgatgtg gtgaccaatg ccaaacggac cctcagggag
      661 ctgaagatcc tcaaacactt caaacacgac aatatcatcg ccatcaagga catcctgaag
      721 cctactgtgc cctatggaga attcagatct gtctatgtgg tactggacct catggagagc
      781 gacctacacc agatcattca ctcttcacag ccgctcaccc tggaacatgt gagatacttc
      841 ctgtaccagc tgcttcgggg cctcaaatac atgcactctg ctcaggtcat ccaccgtgat
      901 cttaaaccct
//
