LOCUS       sequence-368187-        5588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated cytosolic expression of jGCaMP8f under the synapsin 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5588)
  TITLE     GCaMP8/jGCaMP8 AAV plasmids
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-syn-NES-jGCaMP8f-WPRE
FEATURES             Location/Qualifiers
     source          1..5588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        114..561
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      593..602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             650..667
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     949..970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1076..1097)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1337..1356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1928..2516
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1981..2001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2399..2410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2519..2535)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2520..2536)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2565..2686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2576..2595)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2630..2649
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2848..2977
                     /label=AAV2 ITR
     rep_origin      3049..3504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3136..3155)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3346..3367
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3530..3634
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3635..3703,3704..4495)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3853..3872)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4666..5254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5155..5174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(5491..5588,1..32)
                     /label=AAV2 ITR
ORIGIN
        1 gggagtggcc aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta
       61 cttatctacg tagccatgct ctaggaagat ctctgcagag ggccctgcgt atgagtgcaa
      121 gtgggtttta ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac
      181 ccactggaca agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg
      241 aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg
      301 ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg
      361 tcactcgccg gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc
      421 gccggcccag ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat
      481 ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc
      541 gtgtcgtgcc tgagagcgca gtcgaattca agctgctagc aaggatccac ccgccaccat
      601 gggtaacctg aacgaactgg cattgaagtt ggccggtttg gacatcatgc atcatcacca
      661 tcatcacacg cgtcgcaaga agaccttcaa ggaggtggcc accgccgtga agatcatcgc
      721 ccgcctgatg ggcctgaaga tcaacgtcta tatcaaggcc gacaagcaga agaacggcat
      781 caaggcgaac ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
      841 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      901 gagcgtggag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
      961 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
     1021 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1081 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
     1141 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1201 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1261 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
     1321 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1381 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1441 cctggggcac aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga
     1501 atttaaagag gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga
     1561 gctggggacg gtgatgcggt ctctcggact gaaccccaca gaagcagagc tgcaggacat
     1621 gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat
     1681 gatggcaaga aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt
     1741 gtttgataag gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa
     1801 ccttggagag aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga
     1861 tggggatggt caggtaaact acgaagagtt tgtacaaatg atgacagcga agtaaaagct
     1921 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1981 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2041 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2101 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2161 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2221 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2281 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2341 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2401 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2461 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2521 ataccgtcga cctcgacccg ggcggccgct tcgagcagac atgataagat acattgatga
     2581 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2641 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2701 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa
     2761 cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt
     2821 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     2881 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2941 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg gcgtaatagc gaagaggccc
     3001 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     3061 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3121 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3181 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3241 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3301 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3361 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3421 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3481 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3541 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3601 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3661 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3721 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3781 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3841 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3901 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3961 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4021 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4081 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4141 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4201 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4261 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4321 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4381 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4441 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4501 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4801 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5401 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     5461 ccccgcgcgt tggccgattc attaatgcag ctgcgcgctc gctcgctcac tgaggccgcc
     5521 cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg
     5581 cgcagaga
//
