LOCUS       sequence-36821-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Smyth JW, Hong TT, Gao D, Vogan JM, Jensen BC, Fong TS, Simpson PC, 
            Stainier DY, Chi NC, Shaw RM
  TITLE     Limited forward trafficking of connexin 43 reduces cell-cell 
            coupling in stressed human and mouse myocardium.
  JOURNAL   J Clin Invest. 2010 Jan 4. 120(1):266-79.
  PUBMED    20038810
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2010 Jan 4. 120(1):266-79."
COMMENT     Sequence Label: pTRE-TIGHT-Cx43-eYFP
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     223..244
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      286..295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agngctctgg agtaccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
       61 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
      121 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
      181 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      241 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
      301 gtggatcccg ggcccgcggt accgtcgact gcagaattcg aagcttgatc tccaggtcat
      361 caggccgagg tctgctgctg gcacgactgc tggctctgct tgaaggtcgc tggtccacaa
      421 tggctagtgg ctgtaattca tgtccagcag ctagtttttt tgaattctgg ttatcatcgg
      481 ggaaatcaaa aggctgtgca tgggagttag agatggtgct tcccgcctgc cccattcgat
      541 tttgttctgc actgtaatta gcccagtttt gctcacttgc ttgcttgttg taattgcggc
      601 aagaagaatt gtttctgtcg ccagtaacca gcttgtaccc aggaggagac ataggcgaga
      661 ggggagcggt tggtgaggag cagccattga aataagcata tttttgagac ccacagtctt
      721 tggcagggct cagcgcacca ctggtcgcat ggtaagggtc gctctttccc ttaacccgat
      781 ccttaacgcc cttgaagaaa acatagaaga gttcaatgat attcaaggcc agggacacca
      841 aggacaccac cagcatgaag atgatgaaga tggttttctc cgtggggcga gagaggaaac
      901 agtccacctg atgtgggcag ggatctcttt tgcaa
//
