LOCUS       sequence-368224-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains TEVp display cassette, atc inducible (in any E. coli 
            strain) BxbI and arabinose inducible TP901.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Ahan RE, Kirpat BM, Saltepe B, Seker UOS
  TITLE     A Self-Actuated Cellular Protein Delivery Machine.
  JOURNAL   ACS Synth Biol. 2019 Apr 19;8(4):686-696. doi: 
            10.1021/acssynbio.9b00062. Epub 2019 Mar 12.
  PUBMED    30811932
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00062"; journalName: "ACS Synth Biol"; date: 
            "2019-04-19- 19"; volume: "8"; issue: "4"; pages: "686-696"
COMMENT     Sequence Label: pSecEffector
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2..628)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     533..552
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        644..699
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    680..698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1886..1913
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1996..2874)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWAYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2901..3185
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3108..3127
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3239..3256
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4724..4756
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag
                     /note="wild-type LAA variant"
                     /translation="AAKDENYALAA"
     terminator      4771..4857
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(4907..4954)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4950..4968
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(5021..5038)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7538..8245)
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     CDS             complement(8261..8278)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     sig_peptide     complement(8285..8350)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     protein_bind    8401..8458
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    8613..8662
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     terminator      8695..8722
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(8799..8816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8933..9478)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9013..9032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9592..9686)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9707..10366)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10281..10300
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10367..10469)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc
       61 cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata
      121 atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt
      181 gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat
      241 tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact
      301 gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag
      361 cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag
      421 ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc
      481 gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt
      541 tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca
      601 ctttactttt atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcgtt
      661 gatagagtta ttttaccact ccctatcagt gatagagaaa agaattcaaa agatctgaag
      721 gagggtacca tgggaacggt ggcgcagatg gaattagaag cgatcaaaga gcggaaccgt
      781 tcggctgcgc atttcaatat ccgcgccggg aaataccgag gatccctgcc gccgtgggga
      841 tacctgccta cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt gcagcgagag
      901 cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca tctggtggcc
      961 cacgacctga accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca gctgcaaggc
     1021 cgcgagccgc agggccggga gtggtcggct accgcgctga agcgatcgat gatctccggg
     1081 gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc gagacgacga cggagccccg
     1141 ctggtgcggg ctgagccgat cctgacccgt gagcagctgg aggcgctgcg cgccgagctc
     1201 gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct gcgggtgttg
     1261 ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg taagcacccg
     1321 cgctaccgct gccgctcgat ggggttcccg aagcactgcg ggaacggcac ggtggcgatg
     1381 gccgagtggg acgcgttctg cgaggagcag gtactggatc tgctcgggga cgcggagcgt
     1441 ctggagaaag tctgggtagc gggctcggac tccgcggtcg aactcgcgga ggtgaacgcg
     1501 gagctggtgg acctgacgtc gctgatcggc tccccggcct accgggcggg ctctccgcag
     1561 cgagaagcac tggatgcccg tattgcggcg ctggccgcgc ggcaagagga gctggagggc
     1621 ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg ggcagcggtt cggggactgg
     1681 tggcgggagc aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa cgttcggctg
     1741 acgttcgacg tccgcggcgg gctgactcgc acgatcgact tcggggatct tcaggagtac
     1801 gagcagcatc tcaggctcgg cagcgtggtc gaacggctac acaccgggat gtcgtaataa
     1861 tactagatgc attaataatc acactggctc accttcgggt gggcctttct gcgtttatat
     1921 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta tttcctgcag
     1981 ggcggccgcc atatgttatg acaacttgac ggctacatca ttcacttttt cttcacaacc
     2041 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
     2101 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
     2161 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
     2221 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
     2281 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
     2341 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
     2401 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
     2461 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
     2521 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
     2581 aaagtaagcc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
     2641 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
     2701 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     2761 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     2821 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     2881 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
     2941 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat
     3001 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
     3061 cggcagaaaa gtccacattg attatttgca cggcgtcact ctttgctatg ccatagcatt
     3121 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     3181 tccataccgt ttttttgggc tagctactag agtcacacag gaaacctact agatgaaaca
     3241 tcatcaccat caccaccagg ccggcactaa gaaagtagca atctatacac gagtatccac
     3301 tactaaccaa gcagaggaag gcttctcaat tgatgagcaa attgaccgtt taacaaaata
     3361 tgctgaagca atggggtggc aagtatctga tacttatact gatgctggtt tttcaggggc
     3421 caaacttgaa cgcccagcaa tgcaaagatt aatcaacgat atcgagaata aagcttttga
     3481 tacagttctt gtatataagc tagaccgcct ttcacgtagt gtaagagata ctctttatct
     3541 tgttaaggat gtgttcacaa aaaataaaat agactttatc tcgcttaatg aaagtattga
     3601 tacttcttct gctatgggta gcttgtttct cactattctt tctgcaatta atgagtttga
     3661 aagagagaat ataaaagaac gcatgactat gggtaaacta gggcgagcga aatctggtaa
     3721 gtctatgatg tggactaaga cagcttttgg gtattaccac aacagaaaga caggtatatt
     3781 agaaattgtt cctttacaag ctacaatagt tgaacaaata ttcactgatt atttatcagg
     3841 aatatcactt acaaaattaa gagataaact caatgaatct ggacacatcg gtaaagatat
     3901 accgtggtct tatcgtaccc taagacaaac acttgataat ccagtttact gtggttatat
     3961 caaatttaag gacagcctat ttgaaggtat gcacaaacca attatccctt atgagactta
     4021 tttaaaagtt caaaaagagc tagaagaaag acaacagcag acttatgaaa gaaataacaa
     4081 ccctagacct ttccaagcta aatatatgct gtcagggatg gcaaggtgcg gttactgtgg
     4141 agcaccttta aaaattgttc ttggccacaa aagaaaagat ggaagccgca ctatgaaata
     4201 tcactgtgca aatagatttc ctcgaaaaac aaaaggaatt acagtatata atgacaataa
     4261 aaagtgtgat tcaggaactt atgatttaag taatttagaa aatactgtta ttgacaacct
     4321 gattggattt caagaaaata atgactcctt attgaaaatt atcaatggca acaaccaacc
     4381 tattcttgat acttcgtcat ttaaaaagca aatttcacag atcgataaaa aaatacaaaa
     4441 gaactctgat ttgtacctaa atgattttat cactatggat gagttgaaag atcgtactga
     4501 ttcccttcag gctgagaaaa agctgcttaa agctaagatt agcgaaaata aatttaatga
     4561 ctctactgat gtttttgagt tagttaaaac tcagttgggc tcaattccga ttaatgaact
     4621 atcatatgat aataaaaaga aaatcgtcaa caaccttgta tcaaaggttg atgttactgc
     4681 tgataatgta gatatcatat ttaaattcca actcgctacc ggtgctgcta aggacgaaaa
     4741 ctacgctctg gctgcttaaa cgcgtggcat caaataaaac gaaaggctca gtcgaaagac
     4801 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     4861 ccgccctaga cccattcgcc aatccggata tagttcctcc tttcagcaaa aaacccctca
     4921 agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca
     4981 actcagcttc ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct
     5041 cgagtcatca gaaggtcaca ttcagtgtgg cctgaccgtt atacccttca gcgctgctgc
     5101 cgctgacgct gtgggcataa ccggcctgaa cgcccagggt gatattttcc cggacacggg
     5161 cttccagtcc ggcctgcagg tccagtgatg tgccattctg tgagggagag aacgtcatcc
     5221 cgctgcctgc agtggaagtc cccacacgca tatctccccg ggagctgaag gtgcggataa
     5281 cagaaggctg tacccaccag ttcaccggta attcactcac actgtgtttt gcactgtcac
     5341 gcaggggggc acgggatgag gtgccttcgc caaaggtcat atcgttgtgg ctgcccagac
     5401 ggaaaccggc acgcacatgt tgtgcactgc catgcccgaa cttcacataa ccggcgttgt
     5461 ccttaccgtc atccagggaa agtccctgcc aggtatactg cagttgtggc tccagcatca
     5521 ggttgtcagt gatactgaag ggcagaccgg tttccagtga gcccagccag ccccagcccc
     5581 gggcgcggaa gtcgttattg tccgatgacg ctttcatgct gtggcgggtt ccctgtgcca
     5641 caatgtcagc ccacaggccg gaggacgtgt gtaccagatt caggtatccg cccaggctgc
     5701 cggcatcatc ccggaccgtg ccggcacggg agccgtcatc atccttaaca tcaacggaag
     5761 aatggccagc agcaccatat acccccgcgg tcacagacat accggcaacc tctgttctca
     5821 tcaggtcacc ctccagacgg acgaatccat agctgccgct gctttccggc gtggccccac
     5881 gggcaatacc gccattgtta tcgtgaccga gatgaccgcc ctgaatgctg agacggacgc
     5941 tgttgttttc accatttaca ccggtctgat ggctgcggga gcctgccaca atccggtcat
     6001 agtccattgc ctgtgtcagc atggaggcat acagggggac ttctgcacga taagcatttt
     6061 cactgcgcag ataccagctc tcatcactgt cccggttgag ggagtagtta aaggcaccgg
     6121 cctgcagcct gttcccctgg acaaaggccc cttcctccgt ggtggcaccg ttaatggctt
     6181 ccaccacctg aataccctta ccgctggtcg ccagccccga cgcactgttg ccggcgttca
     6241 ccaggttcag gatggttttt ccggttgccc tgccgccgtc aatgaccagt ctgtcagcat
     6301 tgttctgtgc catatccggg cgtacacgca ggctgatggt gccattctgt ccgttcaggt
     6361 ttttcacttt cagggttgcc ggtacgaact tccctgtgcg ggtggaggtg aaatgaatct
     6421 gtccggcatg gctgaggtca tccaccaccg actgcaccgt ggcgttatcg gggatattcc
     6481 aggtggcacc ggaggcgaga gtgacattcg tggggtcaat ggcaccgttc agcacagtgc
     6541 tgccggtcag cttcagggct gtaccgcgct gagcaatgac atccgtggtg acggtactgt
     6601 cgttcagcgt cagcgtgcct tcattcaggt tgacggcttt ctgggtgagt gtggtgttgc
     6661 tgacagtgag tgtgccactt cctgattttt ccacgctgcc gttaccggtg agagagcctc
     6721 cctgcaggag tgcatcgcct tcacggatgg tcagggtatc gttgttcacc gtcttcccgg
     6781 aaagggtaag tatggcgccg ttattcagcg tggtggtgcc cgccagtgtg ccgcccctgg
     6841 cggtgaacag tccgccattt accgtggtat ccgtggccgt atcaccggcg ttcagcgtga
     6901 atgaactgcc tttttccagc atcagggcat ccgcctgacc gcctccgata ctgaatgcct
     6961 ttccgtcgct ccgggtacca ctgacagcgg caccggaatc ggccagcagt acaccgccat
     7021 ttcccatgga tacggtggtg gcggtgccac cgttgcggac atccagcgtt ccgccatcat
     7081 ccacgcgggt attagtggct gtgtgtccgg tcagcacatc caggcgtccg ccattttcca
     7141 gtacgacatt atcagcttta ccctccacaa cagagaatgc tcccaggcgg tttatgccgg
     7201 taacggttgc agccgtactg gtaaccagtg cgccgccctg cttcagggtg acattcgtgg
     7261 ctgtaccacc ggcgtccacc tgcagtctgc ccttctgatt aacggtggta ttcccggcca
     7321 cacccccgtt tttgacaatc tgccagccgt cactgttcac aacagtgtca gacgctgtac
     7381 cgccgttgtg cacatactgg tatcccccat tcagcgtggt atccagtgcg tgaccatgta
     7441 cagtctggtc gccgccggca taaaccacag tggtatttgc cgttccttca gctctcacaa
     7501 tctggcgacc gtttttattg atggttgtgc gcttaagatt catcagctgg gtggcttctt
     7561 tcaccggctg gaacggttct tccggtttca ccatgaacac tttatggccg ccccacagca
     7621 cgctatctgc attcaggcgc caaccgctca cccactgctg tgcttcctgg ttggtcagca
     7681 gttccatgaa gttcttcggc acgctggtaa agtaattatt ggtgttggta aaattactgg
     7741 cactatgaat gcccacgata aagccatcgc gggtactcac cagcgggctg ccacactggc
     7801 catctttggt ttgaatccag tgtttccaga agatgccatc gctgctcgga aaggtgcaac
     7861 tggtatcgct caccatgctg ctcatgcttt tggtctgaaa attggtggtc accagacaaa
     7921 tgcgttcttc gcgctgcggt tcgcggaatt tcagtttctg cggaaacggc gggaagtctt
     7981 tcggcatgcg gatgataatc atgtcgcggc catcgatcag gtgctgctgc agggtggtgg
     8041 tgttcttcac tttgaacacg ccatgcaggc tctgcaccag cagggtgcca ttgttgcggc
     8101 gaaacaggtg tttgttggtg atgataaacg ggccgaagcc gatgccatac aggctggtgg
     8161 tatggccatc actctcgttg gtcaggtggc agatggtact gctgatcggg ttgtaatcgc
     8221 gaggaccctt aaacaggctc tcaccggatc caccgccacc gtggtggtgg tggtggtggc
     8281 ccatggccat cgccggctgg gcagcgagga gcagcagacc agcagcagcg gtcggcagca
     8341 ggtatttcat atgtatatct ccttcttaaa gttaacatgt ggatgggcag tggccggttg
     8401 gtcgtggttt gtctggtcaa ccaccgcggt ctcagtggtg tacggtacaa accccgacgg
     8461 taccacagct aacaccacgt cgtccctatc tgctgcccta ggtctatgag tggttgctgg
     8521 ataactttac gggcatgcat aaggctcgta taatatattc agggagacca caacggtttc
     8581 cctctacaaa taattttgtt taacttttta aggcccggat gatcctgacg acggagaccg
     8641 ccgtcgtcga caagccggcc gaggacgtag ccttcaagtt aatacgttca cactggctca
     8701 ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat
     8761 taatccggct tttttattat ttggatatat tccgcttcct cgctcactga ctcgctacgc
     8821 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     8881 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     8941 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     9001 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     9061 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     9121 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     9181 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     9241 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     9301 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     9361 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     9421 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     9481 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
     9541 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc
     9601 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     9661 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     9721 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     9781 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     9841 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     9901 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     9961 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
    10021 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
    10081 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
    10141 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
    10201 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
    10261 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
    10321 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
    10381 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
    10441 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccagata tcgacgt
//
