LOCUS       sequence-368227-        6833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atc inducible (in any E. coli strain) BxbI and arabinose inducible 
            TP901.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ahan RE, Kirpat BM, Saltepe B, Seker UOS
  TITLE     A Self-Actuated Cellular Protein Delivery Machine.
  JOURNAL   ACS Synth Biol. 2019 Apr 19;8(4):686-696. doi: 
            10.1021/acssynbio.9b00062. Epub 2019 Mar 12.
  PUBMED    30811932
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00062"; journalName: "ACS Synth Biol"; date: 
            "2019-04-19- 19"; volume: "8"; issue: "4"; pages: "686-696"
COMMENT     Sequence Label: pSecEffector wo TEVp
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..42)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(602..696)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(717..1376)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1291..1310
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1377..1479)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      1516..1543
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(1620..1722)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(1752..2378)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     2283..2302
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        2394..2449
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    2430..2448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      3636..3663
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3746..4624)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWAYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4651..4935
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4858..4877
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             4989..5006
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6474..6506
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag
                     /note="wild-type LAA variant"
                     /translation="AAKDENYALAA"
     terminator      6521..6607
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(6642..6659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6776..6833,1..488))
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
       61 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
      121 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
      181 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      241 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
      301 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
      361 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
      421 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
      481 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta
      541 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg
      601 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
      661 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
      721 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      781 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      841 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      901 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
      961 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1021 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1081 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1141 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1201 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1261 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1321 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1381 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1441 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccagata
     1501 tcgacgtctc acactggctc accttcgggt gggcctttct gcgtttatat actagagaga
     1561 gaatataaaa agccagatta ttaatccggc ttttttatta tttcctgcag ggcggccgct
     1621 ttaacttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1681 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag
     1741 atatcgacgt cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc
     1801 aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct
     1861 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
     1921 tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca
     1981 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
     2041 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
     2101 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
     2161 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg
     2221 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
     2281 tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg
     2341 ctgttaatca ctttactttt atctaatcta gacatcatta attcctaatt tttgttgaca
     2401 ctctatcgtt gatagagtta ttttaccact ccctatcagt gatagagaaa agaattcaaa
     2461 agatctgaag gagggtacca tgggaacggt ggcgcagatg gaattagaag cgatcaaaga
     2521 gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag gatccctgcc
     2581 gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt
     2641 gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca
     2701 tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca
     2761 gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga agcgatcgat
     2821 gatctccggg gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc gagacgacga
     2881 cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg aggcgctgcg
     2941 cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct
     3001 gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg
     3061 taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg ggaacggcac
     3121 ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc tgctcgggga
     3181 cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg aactcgcgga
     3241 ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct accgggcggg
     3301 ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc ggcaagagga
     3361 gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg ggcagcggtt
     3421 cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa
     3481 cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact tcggggatct
     3541 tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac acaccgggat
     3601 gtcgtaataa tactagatgc attaataatc acactggctc accttcgggt gggcctttct
     3661 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
     3721 tttcctgcag ggcggccgcc atatgttatg acaacttgac ggctacatca ttcacttttt
     3781 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
     3841 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
     3901 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
     3961 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
     4021 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
     4081 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
     4141 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
     4201 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
     4261 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
     4321 cgcgcggacg aaagtaagcc cactggtgat accattcgcg agcctccgga tgacgaccgt
     4381 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
     4441 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
     4501 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
     4561 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     4621 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     4681 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     4741 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     4801 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcact ctttgctatg
     4861 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     4921 ctactgtttc tccataccgt ttttttgggc tagctactag agtcacacag gaaacctact
     4981 agatgaaaca tcatcaccat caccaccagg ccggcactaa gaaagtagca atctatacac
     5041 gagtatccac tactaaccaa gcagaggaag gcttctcaat tgatgagcaa attgaccgtt
     5101 taacaaaata tgctgaagca atggggtggc aagtatctga tacttatact gatgctggtt
     5161 tttcaggggc caaacttgaa cgcccagcaa tgcaaagatt aatcaacgat atcgagaata
     5221 aagcttttga tacagttctt gtatataagc tagaccgcct ttcacgtagt gtaagagata
     5281 ctctttatct tgttaaggat gtgttcacaa aaaataaaat agactttatc tcgcttaatg
     5341 aaagtattga tacttcttct gctatgggta gcttgtttct cactattctt tctgcaatta
     5401 atgagtttga aagagagaat ataaaagaac gcatgactat gggtaaacta gggcgagcga
     5461 aatctggtaa gtctatgatg tggactaaga cagcttttgg gtattaccac aacagaaaga
     5521 caggtatatt agaaattgtt cctttacaag ctacaatagt tgaacaaata ttcactgatt
     5581 atttatcagg aatatcactt acaaaattaa gagataaact caatgaatct ggacacatcg
     5641 gtaaagatat accgtggtct tatcgtaccc taagacaaac acttgataat ccagtttact
     5701 gtggttatat caaatttaag gacagcctat ttgaaggtat gcacaaacca attatccctt
     5761 atgagactta tttaaaagtt caaaaagagc tagaagaaag acaacagcag acttatgaaa
     5821 gaaataacaa ccctagacct ttccaagcta aatatatgct gtcagggatg gcaaggtgcg
     5881 gttactgtgg agcaccttta aaaattgttc ttggccacaa aagaaaagat ggaagccgca
     5941 ctatgaaata tcactgtgca aatagatttc ctcgaaaaac aaaaggaatt acagtatata
     6001 atgacaataa aaagtgtgat tcaggaactt atgatttaag taatttagaa aatactgtta
     6061 ttgacaacct gattggattt caagaaaata atgactcctt attgaaaatt atcaatggca
     6121 acaaccaacc tattcttgat acttcgtcat ttaaaaagca aatttcacag atcgataaaa
     6181 aaatacaaaa gaactctgat ttgtacctaa atgattttat cactatggat gagttgaaag
     6241 atcgtactga ttcccttcag gctgagaaaa agctgcttaa agctaagatt agcgaaaata
     6301 aatttaatga ctctactgat gtttttgagt tagttaaaac tcagttgggc tcaattccga
     6361 ttaatgaact atcatatgat aataaaaaga aaatcgtcaa caaccttgta tcaaaggttg
     6421 atgttactgc tgataatgta gatatcatat ttaaattcca actcgctacc ggtgctgcta
     6481 aggacgaaaa ctacgctctg gctgcttaaa cgcgtggcat caaataaaac gaaaggctca
     6541 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     6601 gacaaatccg ccgccctaga cctagggata tattccgctt cctcgctcac tgactcgcta
     6661 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     6721 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
     6781 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tgg
//
