LOCUS       sequence-368231-        4719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric hyperfolder YFP fluorescent protein: bacterial expression 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Campbell BC, Paez-Segala MG, Looger LL, Petsko GA, Liu CF
  TITLE     Chemically stable fluorescent proteins for advanced microscopy.
  JOURNAL   Nat Methods. 2022 Nov 7. pii: 10.1038/s41592-022-01660-7. doi: 
            10.1038/s41592-022-01660-7.
  PUBMED    36344833
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Nov 7. pii: 10.1038/s41592-022-01660-7. doi: 
            10.1038/s41592-022-01660-7."
COMMENT     Sequence Label: pBAD-mhYFP
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..308
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     231..250
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             351..368
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(423..444)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1143..1160)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1313..1399
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1491..1518
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1537..1628
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1629..1697,1698..2489)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1847..1866)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2660..3248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3149..3168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3402..3419
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3434..3574
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3500..3519)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3660..3682
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
       61 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
      121 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
      181 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
      241 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
      301 ttctccatac ccgttttttg ggctaacagg aggaattaac catggggagc caccatcacc
      361 atcaccatgg cagatctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      421 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
      481 agggcgatgc caccaacggc aagctgaccc tgaagctcat ctccaccacc ggcaagctgc
      541 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cctgatggtg ttcgcccgct
      601 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      661 aggagcgcac catctctttc gaggacgacg gttactacaa gacccgcgcc gaggtgaagt
      721 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
      781 gcaacatcct ggggcacaag ctggagtaca acttcaaccc ccacaacgtc tatatcacgg
      841 ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac gttgaggacg
      901 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      961 tgatgcccga caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga
     1021 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatga
     1081 acgagctgta caagtaagaa ttcgaagctt ggctgttttg gtggatgaga gaagattttc
     1141 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     1201 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     1261 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     1321 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1381 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     1441 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     1501 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     1561 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1621 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1681 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1741 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1801 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1861 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1921 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     1981 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2041 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2101 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2161 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2221 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2281 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2341 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2401 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2461 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2521 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2581 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2641 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2701 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2761 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2821 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2881 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2941 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3001 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3061 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3121 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3181 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3241 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3301 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3361 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3421 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3481 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     3541 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     3601 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3661 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat
     3721 caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc tgtcaaatgg acgaagcagg
     3781 gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta
     3841 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg
     3901 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac
     3961 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct
     4021 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg
     4081 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct
     4141 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg
     4201 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat
     4261 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
     4321 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat
     4381 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
     4441 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag
     4501 caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc
     4561 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc
     4621 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta
     4681 tcccggcagc aggggatcat tctgcgcttc agccatact
//
