LOCUS       sequence-36827-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 818 (=HL 716 + pHL 98)
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    212..230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    237..255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(276..294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        362..466
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagtcttctc ctttcgcatg ctatcatgaa ctttcagcgt attctgactc ataaggtggc
       61 tcctacccgt gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg
      121 ctgcggcaaa taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa
      181 gcgtgttgaa ctggttccgg tgtcgacagt atctctatca ctgataggga tgtcaatctc
      241 tatcactgat agggactcga ggtgaagacg aaagggcctc gtgatacgcc tatttttata
      301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
//
