LOCUS       sequence-368276-       11488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in maize plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Xu W, Yang Y, Yang B, Krueger CJ, Xiao Q, Zhao S, Zhang L, Kang G, 
            Wang F, Yi H, Ren W, Li L, He X, Zhang C, Zhang B, Zhao J, Yang J
  TITLE     A design optimized prime editor with expanded scope and capability 
            in plants.
  JOURNAL   Nat Plants. 2022 Jan;8(1):45-52. doi: 10.1038/s41477-021-01043-4. 
            Epub 2021 Dec 23.
  PUBMED    34949802
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-01043-4"; journalName: "Nat Plants"; date: 
            "2022-01"; volume: "8"; issue: "1"; pages: "45-52"
COMMENT     Sequence Label: PE-P3-maize
FEATURES             Location/Qualifiers
     source          1..11488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2111..2176
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2183..2203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2213..2233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2243..2263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2273..2293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4445..6958,6959..6961,6962..8545)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5631..5639
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8543..8557
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             8564..8584
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8594..8614
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8624..8644
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8654..8674
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8729..8981
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9416..9438)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9632..9651)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9751..9773
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9904..9923)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9915..10724
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9982..10001)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(10902..11488,1..2)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11391..11410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccggc gccctaggcc
      121 aagcttgtcg tgcccctctc tagagataat gagcattgca tgtctaagtt ataaaaaatt
      181 accacatatt ttttttgtca cacttgtttg aagtgcagtt tatctatctt tatacatata
      241 tttaaacttt actctacgaa taatataatc tatagtacta caataatatc agtgttttag
      301 agaatcatat aaatgaacag ttagacatgg tctaaaggac aattgagtat tttgacaaca
      361 ggactctaca gttttatctt tttagtgtgc atgtgttctc cttttttttt gcaaatagct
      421 tcacctatat aatacttcat ccattttatt agtacatcca tttagggttt agggttaatg
      481 gtttttatag actaattttt ttagtacatc tattttattc tattttagcc tctaaattaa
      541 gaaaactaaa actctatttt agttttttta tttaataatt tagatataaa atagaataaa
      601 ataaagtgac taaaaattaa acaaataccc tttaagaaat taaaaaaact aaggaaacat
      661 ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag tctaacggac
      721 accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc acggcatctc
      781 tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt gctccgctgt
      841 cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca ggcggcctcc
      901 tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc cttcgctttc
      961 ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc ccaacctcgt
     1021 gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg tcggcacctc
     1081 cgcttcaagg tacgccgctc gtcctncccc ccccccctct ctaccttctc tagatcggcg
     1141 ttccggtcca tggttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt
     1201 gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta cgtcagacac
     1261 gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt
     1321 tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc
     1381 cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt
     1441 tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa
     1501 ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca
     1561 tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat
     1621 gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg
     1681 atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa
     1741 ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta
     1801 cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt
     1861 actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac
     1921 ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg
     1981 atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt
     2041 tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcagta
     2101 cgtaagcatg gactacaagg accacgacgg ggattacaaa gaccacgaca tagactacaa
     2161 ggatgacgat gacaaaatgg caccgaagaa aaaaaggaag gtcggcggct ccccgaagaa
     2221 aaaaaggaag gtcggcggct ccccgaagaa aaaaaggaag gtcggcggct ccccgaagaa
     2281 aaaaaggaag gtcggaatcc atggcgttcc agaaaccctc aacatcgagg acgagtacag
     2341 gctccacgag acctccaagg agccggacgt gtccctcggc tccacctggc tctccgactt
     2401 cccgcaggcc tgggccgaga ccggcggcat gggcctcgcc gtgaggcagg ccccgctcat
     2461 catcccgctc aaggccacct ccaccccggt gtccatcaag cagtacccga tgtcccagga
     2521 ggccaggctc ggcatcaagc cgcacatcca gaggctcctc gaccagggca tcctcgtgcc
     2581 gtgccagtcc ccgtggaaca ccccgctcct cccggtgaag aagccgggca ccaacgacta
     2641 caggccggtg caggacctca gggaggtgaa caagagggtg gaggacatcc acccgaccgt
     2701 gccgaacccg tacaacctcc tctccggcct cccgccgtcc caccagtggt acaccgtgct
     2761 cgacctcaag gacgccttct tctgcctcag gctccacccg acctcccagc cgctcttcgc
     2821 cttcgagtgg agggacccgg agatgggcat ctccggccag ctcacctgga ccaggctccc
     2881 gcagggcttc aagaactccc cgaccctctt caacgaggcc ctccacaggg acctcgccga
     2941 cttcaggatc cagcacccgg acctcatcct cctccagtac gtggacgacc tcctcctcgc
     3001 cgccacctcc gagctcgact gccagcaggg caccagggcc ctcctccaga ccctcggcaa
     3061 cctcggctac agggcctccg ccaagaaggc ccagatctgc cagaagcagg tgaagtacct
     3121 cggctacctc ctcaaggagg gccagaggtg gctcaccgag gccaggaagg agaccgtgat
     3181 gggccagccg accccgaaga ccccgaggca gctcagggag ttcctcggca aggccggctt
     3241 ctgcaggctc ttcatcccgg gcttcgccga gatggccgcc ccgctctacc cgctcaccaa
     3301 gccgggcacc ctcttcaact ggggcccgga ccagcagaag gcctaccagg agatcaagca
     3361 ggccctcctc accgccccgg ccctcggcct cccggacctc accaagccgt tcgagctctt
     3421 cgtggacgag aagcagggct acgccaaggg cgtgctcacc cagaagctcg gcccgtggag
     3481 gaggccggtg gcctacctct ccaagaagct cgacccggtg gccgccggct ggccgccgtg
     3541 cctcaggatg gtggccgcca tcgccgtgct caccaaggac gccggcaagc tcaccatggg
     3601 ccagccgctc gtgatcctcg ccccgcacgc cgtggaggcc ctcgtgaagc agccgccgga
     3661 caggtggctc tccaacgcca ggatgaccca ctaccaggcc ctcctcctcg acaccgacag
     3721 ggtgcagttc ggcccggtgg tggccctcaa cccggccacc ctcctcccgc tcccggagga
     3781 gggcctccag cacaactgcc tcgacatcct cgccgaggcc cacggcacca ggccggacct
     3841 caccgaccag ccgctcccgg acgccgacca cacctggtac accgacggct cctccctcct
     3901 ccaggagggc cagaggaagg ccggcgccgc cgtgaccacc gagaccgagg tgatctgggc
     3961 caaggccctc ccggccggca cctccgccca gagggccgag ctcatcgccc tcacccaggc
     4021 cctcaagatg gccgagggca agaagctcaa cgtgtacacc gactccaggt acgccttcgc
     4081 caccgcccac atccacggcg agatctacag gaggaggggc tggctcacct ccgagggcaa
     4141 ggagatcaag aacaaggacg agatcctcgc cctcctcaag gccctcttcc tcccgaagag
     4201 gctctccatc atccactgcc cgggccacca gaagggccac tccgccgagg ccaggggcaa
     4261 caggatggcc gaccaggccg ccaggaaggc cgccatcacc gagaccccgg acacctccac
     4321 cctcctcatc gagaactcct ccccgtccgg cggctcctcc ggcggctcct ccggctccga
     4381 gaccccgggc acctccgagt ccgccacccc ggagtcctcc ggcggctcct ccggcggctc
     4441 ctccgacaag aagtactcca tcggcctcga catcggcacc aacagcgtcg gctgggcggt
     4501 gatcaccgac gagtacaagg tcccgtccaa gaagttcaag gtcctgggca acaccgaccg
     4561 ccactccatc aagaagaacc tcatcggcgc cctcctcttc gactccggcg agacggcgga
     4621 ggcgacccgc ctcaagcgca ccgcccgccg ccgctacacc cgccgcaaga accgcatctg
     4681 ctacctccag gagatcttct ccaacgagat ggcgaaggtc gacgactcct tcttccaccg
     4741 cctcgaggag tccttcctcg tggaggagga caagaagcac gagcgccacc ccatcttcgg
     4801 caacatcgtc gacgaggtcg cctaccacga gaagtacccc actatctacc accttcgtaa
     4861 gaagcttgtt gactctactg ataaggctga tcttcgtctc atctaccttg ctctcgctca
     4921 catgatcaag ttccgtggtc acttccttat cgagggtgac cttaaccctg ataactccga
     4981 cgtggacaag ctcttcatcc agctcgtcca gacctacaac cagctcttcg aggagaaccc
     5041 tatcaacgct tccggtgtcg acgctaaggc gatcctttcc gctaggctct ccaagtccag
     5101 gcgtctcgag aacctcatcg cccagctccc tggtgagaag aagaacggtc ttttcggtaa
     5161 cctcatcgct ctctccctcg gtctgacccc taacttcaag tccaacttcg acctcgctga
     5221 ggacgctaag cttcagctct ccaaggatac ctacgacgat gatctcgaca acctcctcgc
     5281 tcagattgga gatcagtacg ctgatctctt ccttgctgct aagaacctct ccgatgctat
     5341 cctcctttcg gatatcctta gggttaacac tgagatcact aaggctcctc tttctgcttc
     5401 catgatcaag cgctacgacg agcaccacca ggacctcacc ctcctcaagg ctcttgttcg
     5461 tcagcagctc cccgagaagt acaaggagat cttcttcgac cagtccaaga acggctacgc
     5521 cggttacatt gacggtggag ctagccagga ggagttctac aagttcatca agccaatcct
     5581 tgagaagatg gatggtactg aggagcttct cgttaagctt aaccgtgagg acctccttag
     5641 gaagcagagg actttcgata acggctctat ccctcaccag atccaccttg gtgagcttca
     5701 cgccatcctt cgtaggcagg aggacttcta ccctttcctc aaggacaacc gtgagaagat
     5761 cgagaagatc cttactttcc gtattcctta ctacgttggt cctcttgctc gtggtaactc
     5821 ccgtttcgct tggatgacta ggaagtccga ggagactatc accccttgga acttcgagga
     5881 ggttgttgac aagggtgctt ccgcccagtc cttcatcgag cgcatgacca acttcgacaa
     5941 gaacctcccc aacgagaagg tcctccccaa gcactccctc ctctacgagt acttcacggt
     6001 ctacaacgag ctcaccaagg tcaagtacgt caccgagggt atgcgcaagc ctgccttcct
     6061 ctccggcgag cagaagaagg ctatcgttga cctcctcttc aagaccaacc gcaaggtcac
     6121 cgtcaagcag ctcaaggagg actacttcaa gaagatcgag tgcttcgact ccgtcgagat
     6181 cagcggcgtt gaggaccgtt tcaacgcttc tctcggtacc taccacgatc tcctcaagat
     6241 catcaaggac aaggacttcc tcgacaacga ggagaacgag gacatcctcg aggacatcgt
     6301 cctcactctt actctcttcg aggataggga gatgatcgag gagaggctca agacttacgc
     6361 tcatctcttc gatgacaagg ttatgaagca gctcaagcgt cgccgttaca ccggttgggg
     6421 taggctctcc cgcaagctca tcaacggtat cagggataag cagagcggca agactatcct
     6481 cgacttcctc aagtctgatg gtttcgctaa caggaacttc atgcagctca tccacgatga
     6541 ctctcttacc ttcaaggagg atattcagaa ggctcaggtg tccggtcagg gcgactctct
     6601 ccacgagcac attgctaacc ttgctggttc ccctgctatc aagaagggca tccttcagac
     6661 tgttaaggtt gtcgatgagc ttgtcaaggt tatgggtcgt cacaagcctg agaacatcgt
     6721 catcgagatg gctcgtgaga accagactac ccagaagggt cagaagaact cgagggagcg
     6781 catgaagagg attgaggagg gtatcaagga gcttggttct cagatcctta aggagcaccc
     6841 tgtcgagaac acccagctcc agaacgagaa gctctacctc tactacctcc agaacggtag
     6901 ggatatgtac gttgaccagg agctcgacat caacaggctt tctgactacg acgtcgacgc
     6961 cattgttcct cagtctttcc ttaaggatga ctccatcgac aacaaggtcc tcacgaggtc
     7021 cgacaagaac aggggtaagt cggacaacgt cccttccgag gaggttgtca agaagatgaa
     7081 gaactactgg aggcagcttc tcaacgctaa gctcattacc cagaggaagt tcgacaacct
     7141 cacgaaggct gagaggggtg gcctttccga gcttgacaag gctggtttca tcaagaggca
     7201 gcttgttgag acgaggcaga ttaccaagca cgttgctcag atcctcgatt ctaggatgaa
     7261 caccaagtac gacgagaacg acaagctcat ccgcgaggtc aaggtgatca ccctcaagtc
     7321 caagctcgtc tccgacttcc gcaaggactt ccagttctac aaggtccgcg agatcaacaa
     7381 ctaccaccac gctcacgatg cttaccttaa cgctgtcgtt ggtaccgctc ttatcaagaa
     7441 gtaccctaag cttgagtccg agttcgtcta cggtgactac aaggtctacg acgttcgtaa
     7501 gatgatcgcc aagtccgagc aggagatcgg caaggccacc gccaagtact tcttctactc
     7561 caacatcatg aacttcttca agaccgagat caccctcgcc aacggcgaga tccgcaagcg
     7621 ccctcttatc gagacgaacg gtgagactgg tgagatcgtt tgggacaagg gtcgcgactt
     7681 cgctactgtt cgcaaggtcc tttctatgcc tcaggttaac atcgtcaaga agaccgaggt
     7741 ccagaccggt ggcttctcca aggagtctat ccttccaaag agaaactcgg acaagctcat
     7801 cgctaggaag aaggattggg accctaagaa gtacggtggt ttcgactccc ctactgtcgc
     7861 ctactccgtc ctcgtggtcg ccaaggtgga gaagggtaag tcgaagaagc tcaagtccgt
     7921 caaggagctc ctcggcatca ccatcatgga gcgctcctcc ttcgagaaga acccgatcga
     7981 cttcctcgag gccaagggct acaaggaggt caagaaggac ctcatcatca agctccccaa
     8041 gtactctctt ttcgagctcg agaacggtcg taagaggatg ctggcttccg ctggtgagct
     8101 ccagaagggt aacgagcttg ctcttccttc caagtacgtg aacttcctct acctcgcctc
     8161 ccactacgag aagctcaagg gttcccctga ggataacgag cagaagcagc tcttcgtgga
     8221 gcagcacaag cactacctcg acgagatcat cgagcagatc tccgagttct ccaagcgcgt
     8281 catcctcgct gacgctaacc tcgacaaggt cctctccgcc tacaacaagc accgcgacaa
     8341 gcccatccgc gagcaggccg agaacatcat ccacctcttc acgctcacga acctcggcgc
     8401 ccctgctgct ttcaagtact tcgacaccac catcgacagg aagcgttaca cgtccaccaa
     8461 ggaggttctc gacgctactc tcatccacca gtccatcacc ggtctttacg agactcgtat
     8521 cgacctttcc cagcttggtg gtgatgacga tgacaaaatg gcaccgaaga aaaaaaggaa
     8581 ggtcggcggc tccccgaaga aaaaaaggaa ggtcggcggc tccccgaaga aaaaaaggaa
     8641 ggtcggcggc tccccgaaga aaaaaaggaa ggtcggaatc catggcggat caggagccac
     8701 caacttctcc ctcctctagg cggccgccga tcgttcaaac atttggcaat aaagtttctt
     8761 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     8821 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     8881 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     8941 ggataaatta tcgcgcgcgg tgtcatctat gttactagat caattggccc ttacaaaata
     9001 gctagacgtg caggtggctg gatgtgcgct ccctgaatat caacttgtgt ctcctccgat
     9061 tcagtccgca gatgaaactt ggtaataact gcagctgatc cgtcgtcatt catgctatgc
     9121 aggggattcg atcttcagca tgtgcagtgc aggcaacaac aatctacgtt gtctgggctt
     9181 gcgataggta cacgaccacg agggaaggca acgcgtgatg tatgggccgc gcctaagcat
     9241 ccagcccacg cgggcgtgcg cgtcgtcgct acggcttgcg ggggaaggga tcaagggacg
     9301 aaccgagaac tagtaccaga ccggccagcg agcattgcag acaccggctt ataagttcag
     9361 ctgcgaccac cgctccggcg cgccatcgtc cgcctgcagg gaattctctg gccgtcgttt
     9421 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9481 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9541 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     9601 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9661 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9721 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9781 cgtcatcacc gaaacgcgcg atgcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     9841 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     9901 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     9961 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
    10021 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
    10081 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
    10141 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
    10201 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
    10261 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
    10321 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
    10381 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
    10441 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
    10501 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
    10561 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
    10621 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
    10681 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
    10741 ggttgtaaca ttattcagat tgggcttgat ttaaaacttc atttttaatt taaaaggatc
    10801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    11161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    11221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11461 tgctcgtcag gggggcggag cctatgga
//
