LOCUS       sequence-368280-        8089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  arabinose inducible luxI, AHL inducible tetR, constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Fedorec AJH, Karkaria BD, Sulu M, Barnes CP
  TITLE     Single strain control of microbial consortia.
  JOURNAL   Nat Commun. 2021 Mar 30;12(1):1977. doi: 10.1038/s41467-021-22240-x.
  PUBMED    33785746
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-30"; volume: "12(1):1977. doi"; pages: " 
            10.1038/s41467-021-22240-x"
COMMENT     Sequence Label: p63_AF043
FEATURES             Location/Qualifiers
     source          1..8089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(27..113)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     128..150
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        178..212
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             221..232
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2002..2625
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2075..2094)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(2738..3616)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3643..3927
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3850..3869
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3970..3987
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3997..4698
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     RBS             4708..4719
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5362..5456
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(5487..5492,5493..5509,5510..5515)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5704..6237
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            6448..6556
                     /note="incP origin of transfer"
     CDS             complement(6567..7229)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7230..8001
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
       61 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctt
      121 taattaaagc ggataacaat ttcacacagg aggccgccta gtaacgatgg ttggctgttt
      181 acagctagct cagtgctagg tattatgcta gctactagag aaagaggaga aatactagat
      241 gagcaagggc gaggagaaca acctggccat catcaaggag ttcatgcgct tcaaggtaca
      301 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      361 gtatgaaggc acccagaccg cgaaactgaa agtgaccaaa ggtggcccgc tgccgttcgc
      421 gtgggatatt ctgagccctc agtttatgta tggcagcaaa gcgtatgtga aacatccggc
      481 ggatattccg gattatctga aactgagctt cccggaaggc ttcaactggg aacgcgtgat
      541 gaacttcgaa gatggcggcg tggtgaccgt gacccaggat agcagcttgc aggatggcga
      601 gttcatctac aaggtgaagc tgcgcggcac caactttccg agcgatggcc cggtaatgca
      661 gtgccgcacg atgggctggg aagcgagcac cgaacgcatg tatccggaag atggcgcgct
      721 gaaaggcgaa attaaacagc gcctgaaact gaaggatggc ggccattatg atgcggaagt
      781 gaaaaccacc tataaagcga agaaaccggt gcagctgccg ggcgcgtata acgtggatat
      841 taaactggat attctgagcc ataacgaaga ttataccatt gtggaacagt atgaacgcgc
      901 ggaaggccgc catagcaccg gcggcatgga tgaactgtat aaataacgat gcgcgcaata
      961 aaaaagcccc cggaaggtga tcttccgggg gctttctcat gcgttcaatt gctcgagtta
     1021 atttttaaag tatgggcaat caattgctcc tgttaaaatt gctttagaaa tactttggca
     1081 gcggtttgtt gtattgagtt tcatttgcgc attggttaaa tggaaagtga cagtacgctc
     1141 actgcagcct aatatttttg aaatatccca agagcttttt ccttcgcatg cccacgctaa
     1201 acattctttt tctcttttgg ttaaatcgtt gtttgattta ttatttgcta tatttatttt
     1261 tcgataatta tcaactagag aaggaacaat taatggtatg ttcatacacg catgtaaaaa
     1321 taaactatct atatagttgt ctttttctga atgtgcaaaa ctaagcattc cgaagccatt
     1381 gttagccgta tgaataggga aactaaaccc agtgataaga cctgatgttt tcgcttcttt
     1441 aattacattt ggagattttt tatttacagc attgttttca aatatattcc aattaattgg
     1501 tgaatgattg gagttagaat aatctactat aggatcatat tttattaaat tagcgtcatc
     1561 ataatattgc ctccattttt tagggtaatt atctagaatt gaaatatcag atttaaccat
     1621 agaatgagga taaatgatcg cgagtaaata atattcacaa tgtaccattt tagtcatatc
     1681 agataagcat tgattaatat cattattgct tctacaagct ttaattttat taattattct
     1741 gtatgtgtcg tcggcattta tgtttttcat acccatctct ttatccttac ctattgtttg
     1801 tcgcaagttt tgcgtgttat atatcattaa aacggtaatg gattgacatt tgattctaat
     1861 aaattggatt tttgtcacac tattgtatcg ctgggaatac aattacttaa cataagcacc
     1921 tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaatg ggccccggcg
     1981 aagctaggga cgagagctag catgtctcgt ttagataaaa gtaaagtgat taacagcgca
     2041 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
     2101 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
     2161 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
     2221 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
     2281 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
     2341 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
     2401 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
     2461 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
     2521 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
     2581 atctgcggat tagaaaaaca acttaaatgt gaaagtgggt cttgagagct cggactgcct
     2641 gcaggcatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
     2701 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
     2761 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     2821 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     2881 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     2941 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     3001 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     3061 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     3121 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     3181 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     3241 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     3301 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     3361 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     3421 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     3481 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     3541 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     3601 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     3661 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg
     3721 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     3781 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     3841 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     3901 tatcgcaact ctctactgtt tctccatacc cgttttttgg gctaacagga ggaattaacc
     3961 atggggagcc accatcacca tcaccatggc agatctatga gcgagctgat taaggagaac
     4021 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     4081 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     4141 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     4201 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     4261 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     4321 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     4381 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     4441 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     4501 catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     4561 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     4621 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     4681 gggcacaagc ttaattaaga attcattaaa gaggagaaag gtaccatgac tataatgata
     4741 aaaaaatcgg attttttggc aattccatcg gaggagtata aaggtattct aagtcttcgt
     4801 tatcaagtgt ttaagcaaag acttgagtgg gacttagttg tagaaaataa ccttgaatca
     4861 gatgagtatg ataactcaaa tgcagaatat atttatgctt gtgatgatac tgaaaatgta
     4921 agtggatgct ggcgtttatt acctacaaca ggtgattata tgctgaaaag tgtttttcct
     4981 gaattgcttg gtcaacagag tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt
     5041 gctgtaggta aaaatagctc aaagataaat aactctgcta gtgaaattac aatgaaacta
     5101 tttgaagcta tatataaaca cgctgttagt caaggtatta cagaatatgt aacagtaaca
     5161 tcaacagcaa tagagcgatt tttaaagcgt attaaagttc cttgtcatcg tattggagac
     5221 aaagaaattc atgtattagg tgatactaaa tcggttgtat tgtctatgcc tattaatgaa
     5281 cagtttaaaa aagcagtctt aaatgcagcg aacgacgaaa attacgccct tgcagcgtaa
     5341 acgcgtgcta gactagtctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     5401 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccag
     5461 gggtccccaa taattacgat ttaaatttga cataagcctg ttcggttcgt aaactgtaat
     5521 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     5581 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag cctatgcctc
     5641 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     5701 acgatgttac gcagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     5761 ggtggctcaa gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc
     5821 atgcgggctg ctcttgatct tttcggtcgt gagttcggag acgtagccac ctactcccaa
     5881 catcagccgg actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt
     5941 gctgccttcg accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaagttt
     6001 gagcagccgc gtagtgagat ctatatctat gatctcgcag tctccggaga gcaccggagg
     6061 cagggcattg ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct
     6121 tatgtgatct acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag
     6181 ttgggcatac gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaacaa
     6241 ttcgttcaag ccgagatcgg cttcccggcc gcggagttgt tcggtaaatt ggacaacggt
     6301 ccgcgcgttg tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc
     6361 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
     6421 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
     6481 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
     6541 tctgcgaggc tggccgtagg ccggccctac cggcgcggca gcgttacccg tgtcggcggc
     6601 tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag gcgctgctgc
     6661 ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc catgcccgga
     6721 atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg ctcgcccgga
     6781 tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc ctggtcgtcg
     6841 tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg gggctggccc
     6901 cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt gagcttcacg
     6961 acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt ccgcaaagaa
     7021 cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg gtggcccatc
     7081 tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat aagcccggcc
     7141 cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt cttggcttga
     7201 atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg gtgccgcacg
     7261 gttgcggcac catgcgcaat cagctgcaac ttttcggcag cgcgacaaca attatgcgtt
     7321 gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta cgcaatgagc tattgcgggg
     7381 ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt tgttgatttt taagtctttc
     7441 gcatttcgcc ctatatctag ttctttggtg cccaaagaag ggcacccctg cggggttccc
     7501 ccacgccttc ggcgcggctc cccctccggc aaaaagtggc ccctccgggg cttgttgatc
     7561 gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc cccgcactcg
     7621 ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc ttcgactgcc cccactcgca
     7681 taggcttggg tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc tgcgcgagcc
     7741 ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc gcaggccgga
     7801 ggcttttccc cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc cgcgcagttg
     7861 gagccggtgg gtatgtggtc gaaggctggg tagccggtgg gcaatccctg tggtcaagct
     7921 cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg gttgtccacg ggccgagcga
     7981 agcgagccag ccggtggccg ctcgcggcca tcgtccacat atccacgggc tggcaaggga
     8041 gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtagggggg
//
