LOCUS       sequence-368298-          88 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for yeast expression of Cas9; contains a golden gate cloning
            site to additionally express a gRNA for a target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 88)
  AUTHORS   Aguilar RR, Shen ZJ, Tyler JK
  TITLE     A Simple, Improved Method for Scarless Genome Editing of Budding 
            Yeast Using CRISPR-Cas9.
  JOURNAL   Methods Protoc. 2022 Oct 4;5(5):79. doi: 10.3390/mps5050079.
  PUBMED    36287051
REFERENCE   2  (bases 1 to 88)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Protoc."; date: "2022-10-4"; volume: "5(5):79. doi"; pages: " 
            10.3390/mps5050079"
COMMENT     Sequence Label: pCASB
FEATURES             Location/Qualifiers
     source          1..88
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caccttcggg tggcgaatgg gactttagag acccataata acgtccaata aatggtctca
       61 gttttagagc tagaaatagc aagttaaa
//
