LOCUS       sequence-36831-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2300 (=HL 716 + pHL 745)
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    231..247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        305..409
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(628..647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agtcttctcc tttcgcatgc tcgcggtacc tttaatcagg ttgttcaggt tagtgacata
       61 agtctgatgg tgcttgccgt agtgatactc gatggtttcc gcagaaatgt gcggtgccag
      121 agcatcttta gcatatggta gtgcaggtaa ttcgaatgac attgctactc tcctttatta
      181 ttaatttgca tacgtacaat agccttattg tgcgtatggt cgacagtatc ttgttatccg
      241 ctcacaatgt caatgttatc cgctcacatt tatgacgtca ggtggcactt ttcggggaaa
      301 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      361 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      421 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      481 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      541 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      601 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatc
//
