LOCUS       sequence-368325-       14124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses POI-PUP-IT fusion in Plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Ye R, Wang M, Du H, Chhajed S, Koh J, Liu KH, Shin J, Wu Y, Shi L, 
            Xu L, Chen S, Zhang Y, Sheen J
  TITLE     Glucose-driven TOR-FIE-PRC2 signalling controls plant development.
  JOURNAL   Nature. 2022 Sep 14. pii: 10.1038/s41586-022-05171-5. doi: 
            10.1038/s41586-022-05171-5.
  PUBMED    36104568
REFERENCE   2  (bases 1 to 14124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Sep 14. pii: 10.1038/s41586-022-05171-5. doi: 
            10.1038/s41586-022-05171-5."
COMMENT     Sequence Label: pCambia-PUP-IT
FEATURES             Location/Qualifiers
     source          1..14124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    166..190
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    268..442
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(483..1508)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1575..2251)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    2442..2463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2478..2483,2484..2501,2502..2508)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2516..2532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2521..2543
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2540..2556
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2540..2556
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2571..2915
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2948..2965
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2966..3031
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3244..3496
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             6238..6264
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6268..6294
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      6303..6555
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6592..6609)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6592..6608)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6601..6623)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6811..6835
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8136..8765
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9194..10267
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10333..10527
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10763..10785)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10871..11011
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10926..10945
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11026..11043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11197..11785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11277..11296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13071..13865)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
       61 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
      121 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
      181 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
      241 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag
      301 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
      361 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
      421 ctcacacatt attatggaga aactcgagct tgtcgatcga cagatcccgg tcggcatcta
      481 ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
      541 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
      601 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
      661 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
      721 cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
      781 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
      841 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
      901 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
      961 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     1021 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     1081 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     1141 gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc
     1201 aggtcttgca acgtgacacc ctgtgaacgg cgggagatgc aataggtcag gctctcgcta
     1261 aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     1321 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     1381 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     1441 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     1501 tttttcatat ctcattgccc cccggatctg cgaaagctcg agagagatag atttgtagag
     1561 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
     1621 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
     1681 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
     1741 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
     1801 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
     1861 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     1921 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     1981 gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     2041 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     2101 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     2161 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     2221 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     2281 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
     2341 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2401 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2461 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2521 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc tgagactttt
     2581 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     2641 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
     2701 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     2761 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     2821 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     2881 tctatataag gaagttcatt tcatttggag aggacgtacc cggggatccg gtaggatgcg
     2941 tggatctcat catcatcatc atcatgacta caaagaccat gacggtgatt ataaagatca
     3001 tgacatcgac tacaaggatg acgatgacaa gtctcaagat cctatgaacg caaagcaaac
     3061 ccaaattatg ggtggcggcg gacgtgacga ggacaacgcc gaggattccg cacaggcatc
     3121 tggacaggtt cagatcaaca ccgaaggtgt ggacagcttg ctggatgaaa tcgacggact
     3181 gttggaaaac aacgccgagg aattcgttcg ttcctatgta caaaagggtg gcgaatgact
     3241 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     3301 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     3361 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     3421 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     3481 tctatgttac tagatctcat tcttgttaca ttgttattaa tgaaaaaata ttattggtca
     3541 ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca ttgatatatg
     3601 aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa cgagacttgt
     3661 tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac aaaaatatcc
     3721 aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac gataaagaag
     3781 ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata aatggcaaaa
     3841 aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga aatacgcttc
     3901 aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt atatttaaaa
     3961 aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct caacggctgg
     4021 atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa
     4081 gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct
     4141 caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca
     4201 cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc
     4261 ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa
     4321 ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca
     4381 aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc
     4441 aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg
     4501 ggtttgatcg ttagatatca tcttaattct cgattagggt ttcataaata tcatccgatt
     4561 tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga
     4621 tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag tttttctgat
     4681 taacagatga gtaccgtgga atccgcattg acccgcagga tcatgggcat tgaaacggag
     4741 tatggcctca cctttgttga tggtgattcc aaaaagcttc gcccagatga gatagctcga
     4801 aggatgtttc gtcccatcgt ggagaaatat tccagctcta atatcttcat acccaatggt
     4861 tcccggttgt atcttgatgt gggttcccac ccggagtacg ccaccgccga gtgtgataat
     4921 ttgacccagc tgatcaattt tgaaaaagct ggcgatgtta ttgcagatcg catggctgta
     4981 gatgccgaag agtcgctggc gaaagaagac attgctgggc aggtgtacct gtttaaaaac
     5041 aatgtcgatt ccgtgggcaa ttcttatggc tgccacgaaa actaccttgt gggtcgctcc
     5101 atgccgttga aggcgttggg taaaaggctg atgccgtttc tgattacccg ccagctcatc
     5161 tgcggcgccg gcaggatcca tcaccccaat cctttggata aaggcgaatc cttccccttg
     5221 ggctactgca tatcccagcg ctctgaccac gtgtgggagg gcgtatcaag tgccaccact
     5281 agatcacgcc ccattatcaa cacccgtgat gagccacatg cggattccca ttcttaccgc
     5341 aggctgcacg tgattgtggg tgatgccaac atggcagagc ccagcatcgc gttgaaggtc
     5401 ggctccacgt tgctggttct ggaaatgatt gaggcagatt tcggtttgcc cagcttagag
     5461 cttgccaatg atattgcctc aattagggaa atctcccgcg atgcaacagg atccacactg
     5521 ttgtccctga aagatggcac caccatgact gccttgcaga tccagcaggt ggtctttgag
     5581 catgcctcga agtggttgga gcagcgcccc gaaccagaat tttctggcac ctccaacaca
     5641 gagatggccc gcgtgctgga tctgtggggt cgcatgttga aagcgattga gtccggtgat
     5701 ttcagcgaag tggatacaga aattgactgg gtgatcaaaa agaagctcat tgatcgtttc
     5761 attcagcgcg gcaaccttgg gttggatgat ccaaaacttg cccaagtgga cttgacttat
     5821 cacgatatta ggccaggtag aggcctattt agcgtgctgc aaagccgcgg catgatcaaa
     5881 cggtggacta ctgatgaggc gattttagct gcggtggata ccgctcctga tacaacacgt
     5941 gctcatttgc gcgggcgaat ccttaaagcg gcggatactc tgggagtacc tgtgactgtc
     6001 gattggatgc gtcacaaggt caaccgaccg gagccacaat cggtggaatt gggggatcct
     6061 ttttccgcag tgaattccga ggttgatcag ttgattgagt atatgaccgt tcatgcggag
     6121 agctatcgca gtcaattggg aggcggaggt tctggcggtg gtggtactag tggtggaggt
     6181 ggtagcggtg gcggagggag cggttccggc tctcctagga atccatgggg ttctccttac
     6241 ccatacgacg ttccagacta cgctggttac ccatacgacg ttccagacta cgcttgactg
     6301 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     6361 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     6421 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     6481 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     6541 ctatgttact agatccgctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt
     6601 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6661 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6721 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     6781 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     6841 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     6901 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     6961 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     7021 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     7081 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     7141 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     7201 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     7261 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     7321 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     7381 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     7441 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     7501 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     7561 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     7621 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     7681 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     7741 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     7801 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     7861 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     7921 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     7981 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     8041 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     8101 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     8161 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     8221 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     8281 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     8341 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     8401 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     8461 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     8521 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     8581 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     8641 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     8701 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     8761 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     8821 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     8881 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     8941 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     9001 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     9061 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     9121 ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc
     9181 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     9241 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     9301 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     9361 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     9421 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     9481 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     9541 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     9601 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     9661 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     9721 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     9781 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     9841 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     9901 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     9961 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
    10021 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
    10081 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
    10141 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
    10201 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
    10261 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
    10321 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
    10381 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
    10441 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
    10501 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
    10561 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
    10621 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
    10681 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
    10741 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10801 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10861 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
    10921 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10981 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
    11041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    11641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11821 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggg aaggtgcgaa
    11881 caagtccctg atatgagatc atgtttgtca tctggagcca tagaacaggg ttcatcatga
    11941 gtcatcaact taccttcgcc gacagtgaat tcagcagtaa gcgccgtcag accagaaaag
    12001 agattttctt gtcccgcatg gagcagattc tgccatggca aaacatggtg gaagtcatcg
    12061 agccgtttta ccccaaggct ggtaatggcc ggcgacctta tccgctggaa accatgctac
    12121 gcattcactg catgcagcat tggtacaacc tgagcgatgg cgcgatggaa gatgctctgt
    12181 acgaaatcgc ctccatgcgt ctgtttgccc ggttatccct ggatagcgcc ttgccggacc
    12241 gcaccaccat catgaatttc cgccacctgc tggagcagca tcaactggcc cgccaattgt
    12301 tcaagaccat caatcgctgg ctggccgaag caggcgtcat gatgactcaa ggcaccttgg
    12361 tcgatgccac catcattgag gcacccagct cgaccaagaa caaagagcag caacgcgatc
    12421 cggagatgca tcagaccaag aaaggcaatc agtggcactt tggcatgaag gcccacattg
    12481 gtgtcgatgc caagagtggc ctgacccaca gcctggtcac caccgcggcc aacgagcatg
    12541 acctcaatca gctgggtaat ctgctgcatg gagaggagca atttgtctca gccgatgccg
    12601 gctaccaagg ggcgccacag cgcgaggagc tggccgaggt ggatgtggac tggctgatcg
    12661 ccgagcgccc cggcaaggta agaaccttga aacagcatcc acgcaagaac aaaacggcca
    12721 tcaacatcga atacatgaaa gccagcatcc gggccagggt ggagcaccca tttcgcatca
    12781 tcaagcgaca gttcggcttc gtgaaagcca gatacaaggg gttgctgaaa aacgataacc
    12841 aactggcgat gttattcacg ctggccaacc tgtttcgggc ggaccaaatg atacgtcagt
    12901 gggagagatc tcactaaaaa ctggggataa cgccttaaat ggcgaagaaa cggtctaaat
    12961 aggctgattc aaggcattta cgggagaaaa aatcggctca aacatgaaga aatgaaatga
    13021 ctgagtcagc cgagaagaat ttccccgctt attcgcacct tccctaggta ctaaaacaat
    13081 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
    13141 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
    13201 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
    13261 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
    13321 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
    13381 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    13441 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    13501 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    13561 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    13621 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    13681 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    13741 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    13801 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    13861 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    13921 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
    13981 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    14041 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    14101 tccgcgagat catccgtgtt tcaa
//
