LOCUS       sequence-36835-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2304 (=HL 716 + pHL 269)
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        227..331
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(550..569)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaagtcttct cctttcgcat gctagctggg accaggaggg acagtacttt aactttcatg
       61 ttattaaccc tctgttatat gcctttattt gcttttttat gccactgcat actgattaac
      121 cctcattaat cagtcggcaa gtcgacagta tcttgttatc cgctcacaat gtcaatgtta
      181 tccgctcaca tttatgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      541 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      661 tcttacggat ggcatgacag taagaga
//
