LOCUS       sequence-368357-        4752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mitochondrial labeling with blue fluorescent protein Electra2 fused 
            to partial COX8A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, 
            Jia JM, Koster RW, Namikawa K, Piatkevich KD
  TITLE     Dual-expression system for blue fluorescent protein optimization.
  JOURNAL   Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0.
  PUBMED    35715437
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-06-17"; volume: "12(1):10190. doi"; pages: " 
            10.1038/s41598-022-13214-0"
COMMENT     Sequence Label: pMito-Electra2
FEATURES             Location/Qualifiers
     source          1..4752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      158..293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             342..1136
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             342..1136
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(396..415)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1006..1025
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1324..1343)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1368..1415
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1744..2332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2233..2252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2507..2810
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2811..3014
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2985
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3043..3117
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3145..3154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3986..4107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4023..4042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4077..4096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4114..4569)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4251..4272)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4463..4482
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4596..4700
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4702..4752,1..307)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(4702..4752,1..141)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4725..4745)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
       61 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      121 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
      241 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      301 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
      361 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      421 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      481 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
      541 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
      601 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
      661 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
      721 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
      781 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
      841 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
      901 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
      961 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     1021 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     1081 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     1141 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     1201 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     1261 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     1321 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     1381 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     1441 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     1501 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     1561 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     1621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2401 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
     2461 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2521 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2581 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2641 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2701 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2761 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2821 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2881 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2941 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3001 atataagcag agctggttta gtgaaccgtc agatccgcta gcatgtccgt cctgacgccg
     3061 ctgctgctgc ggggcttgac aggctcggcc cggcggctcc cagtgccgcg cgccaagatc
     3121 cattcgttgg gggatccacc ggtcgccacc atggtgtcta agggcgaaga gctgatcgag
     3181 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc
     3241 acaggtgaag gagaaggcag accgtacgag ggagtgcaaa ccatgaggat caaagtcatc
     3301 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcct gttcggcagc
     3361 aagaccttta tcaagtaccc ggccgacatc cctgatttct tcgaacagtc ctttcctgag
     3421 ggctttactt gggaaagagt tacgagatac gaagatggtg gagtagtcac cgtcacgcag
     3481 gacaccagcc ttgaggatgg tgggctcgtc tacaacgtca aggtcagagg ggtaaacttt
     3541 cactccaagg gtcccgtgat gcagaagaag accgagggtt gggagccttt cacagagatg
     3601 atgtatccag cagatggtgg tctgagagga tataccgaca tcgcactgaa agttgatggt
     3661 ggtggccatc tgcacgccaa catcgtgaca acttacaggt caaaaaagac cgtcgggaac
     3721 atcaagatgc ccggtgtcca tgccgttgat taccgcctgg aaaggatcga agagagtgac
     3781 aatgaaacct acgtagtgct aagagaagtg gcagttgcca aatacagcaa ccttggtggt
     3841 ggcatggacg agctgttcaa gtaagcggcc gcgactctag atcataatca gccataccac
     3901 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3961 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4021 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4081 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     4141 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4201 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     4261 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4321 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4381 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4441 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4501 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4561 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4621 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4681 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     4741 agggtgtgga aa
//
