LOCUS       sequence-368364-       10081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for copper-inducible expression of nanoluc luciferase and 
            firefly luciferase driven by minimal synthetic promoters with 
            binding sites for CUP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: pEPCT-Omega-SP0022
FEATURES             Location/Qualifiers
     source          1..10081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..42
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1343..1972
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2401..3474
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3540..3734
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3970..3992)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4078..4218
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4133..4152
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4233..4250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4404..4992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4484..4503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5080..5871)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6657..6681
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6888..6934
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    6950..7005
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             7007..7519
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             7580..7603
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    7641..7815
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        8011..8057
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    8073..8128
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             8130..9779
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8196..8214)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             9843..9866
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    9904..10078
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 gtcatgagac cggatcctga caggatatat tggcgggtaa acctaagaga aaagagcgtt
       61 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
      121 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
      181 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg
      241 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
      301 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
      361 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
      421 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
      481 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
      541 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
      601 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
      661 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
      721 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
      781 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
      841 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
      901 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
      961 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     1021 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     1081 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     1141 acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     1201 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     1261 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     1321 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     1381 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     1441 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     1501 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     1561 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     1621 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     1681 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     1741 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     1801 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     1861 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     1921 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     1981 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     2041 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     2101 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     2161 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     2221 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     2281 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     2341 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     2401 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     2461 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     2521 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     2581 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     2641 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     2701 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     2761 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     2821 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     2881 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     2941 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     3001 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     3061 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     3121 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     3181 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     3241 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     3301 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     3361 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     3421 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     3481 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg actctttcct
     3541 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     3601 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     3661 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     3721 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     3781 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     3841 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     3901 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     3961 cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga
     4021 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     4081 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     4141 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     4201 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4321 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4561 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4801 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5041 aactcacgtt aagggatttt ggtcatgcat tctaggtgat tatttgccga ctaccttggt
     5101 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     5161 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     5221 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     5281 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcac cagcccagtc
     5341 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     5401 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     5461 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     5521 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     5581 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     5641 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     5701 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     5761 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     5821 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca taatgtttaa
     5881 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     5941 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     6001 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     6061 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct tacgaaccga
     6121 acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     6181 aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg ctggaacaga acttattatt
     6241 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
     6301 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
     6361 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
     6421 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
     6481 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
     6541 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
     6601 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
     6661 aggatatatt gtggtgtaaa cataacggat ccggtctcag gagagcgatg cgtcttttcc
     6721 gatgaaccgt tccagcaaaa aagactaagc gatgcgtctt ttccgctgaa ccgttccagc
     6781 aaaaaagact aagcgatgcg tcttttccgc tgaaccgttc cagcaaaaaa gactaggaga
     6841 gcgatgcgtc ttttccgctg aaccgttcca gcaaaaaaga ctacttcgca agacccttcc
     6901 tctatataag gaagttcatt tcatttggag aggatactgt atttttacaa caattaccaa
     6961 caacaacaaa caacaaacaa cattacaatt actatttaca attacaatgg tattcacact
     7021 cgaagatttc gttggggact ggcgacagac agccggctac aacctggacc aagtccttga
     7081 acagggaggt gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc cgatccaaag
     7141 gattgtcctg agcggtgaaa atgggctgaa gatcgacatc catgtcatca tcccgtatga
     7201 aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt
     7261 ggatgatcat cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac
     7321 gccgaacatg atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa
     7381 aaagatcact gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat
     7441 caaccccgac ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct
     7501 gtgcgaacgc attctggctt cggattataa ggaccatgac ggagactata aggaccatga
     7561 cctcgacgct gcagcagcgg attataagga cgatgacgat aagtgagctt ctctagctag
     7621 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     7681 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     7741 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     7801 actaaaatcc agatcgctgt caggagagcg atgcgtcttt tccgatgaac cgttccagca
     7861 aaaaagacta agcgatgcgt cttttccgct gaaccgttcc agcaaaaaag actaagcgat
     7921 gcgtcttttc cgctgaaccg ttccagcaaa aaagactagg agagcgatgc gtcttttccg
     7981 ctgaaccgtt ccagcaaaaa agactacttc gcaagaccct tcctctatat aaggaagttc
     8041 atttcatttg gagaggatac tgtattttta caacaattac caacaacaac aaacaacaaa
     8101 caacattaca attactattt acaattacaa tggaggacgc caaaaacata aagaaaggcc
     8161 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
     8221 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
     8281 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
     8341 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
     8401 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
     8461 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
     8521 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
     8581 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
     8641 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
     8701 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
     8761 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
     8821 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
     8881 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
     8941 aactgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
     9001 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
     9061 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
     9121 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
     9181 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
     9241 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
     9301 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
     9361 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaggacgaac
     9421 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     9481 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     9541 gtctgcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     9601 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     9661 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     9721 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgg
     9781 gttcggatta taaggaccat gacggagact ataaggacca tgacctcgac gctgcagcag
     9841 cggattataa ggacgatgac gataagtgag cttctctagc tagagtcgat cgacaagctc
     9901 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     9961 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
    10021 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatcgc
    10081 t
//
