LOCUS       sequence-368369-       10501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for constitutive expression of nanoluc luciferase and firefly
            luciferase driven by 35s promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 10501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: pEPCT-Omega-SP0018
FEATURES             Location/Qualifiers
     source          1..10501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..87
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    125..299
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    320..344
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1645..2274
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2703..3776
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3842..4036
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4272..4294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4380..4520
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4435..4454
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4535..4552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4706..5294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4786..4805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5382..6173)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6959..6983
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        7083..7428
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             7519..8031
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             8092..8115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    8153..8327
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        8416..8761
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8852..10501
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8918..8936)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggttcggatt ataaggacca tgacggagac tataaggacc atgacctcga cgctgcagca
       61 gcggattata aggacgatga cgataagtga gcttctctag ctagagtcga tcgacaagct
      121 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
      181 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
      241 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg
      301 ctgtcatgag accggatcct gacaggatat attggcgggt aaacctaaga gaaaagagcg
      361 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
      421 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
      481 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt
      541 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
      601 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
      661 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
      721 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
      781 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
      841 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
      901 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
      961 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     1021 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     1081 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     1141 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     1201 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     1261 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     1321 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     1381 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     1441 gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     1501 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     1561 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     1621 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     1681 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     1741 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     1801 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     1861 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     1921 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     1981 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     2041 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     2101 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     2161 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     2221 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     2281 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     2341 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     2401 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     2461 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     2521 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     2581 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     2641 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     2701 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     2761 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     2821 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     2881 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     2941 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     3001 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     3061 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     3121 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     3181 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     3241 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     3301 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     3361 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     3421 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     3481 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     3541 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     3601 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     3661 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     3721 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     3781 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc
     3841 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     3901 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     3961 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     4021 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     4081 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     4141 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     4201 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     4261 gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg
     4321 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     4381 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     4441 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     4501 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     5161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5341 aaaactcacg ttaagggatt ttggtcatgc attctaggtg attatttgcc gactaccttg
     5401 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5461 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5521 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5581 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc accagcccag
     5641 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5701 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5761 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5821 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5881 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5941 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     6001 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     6061 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     6121 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct cataatgttt
     6181 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     6241 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6301 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6361 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6421 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     6481 gcaaccttgg gtagcagcga agtcgaggca tttctgtcct ggctggaaca gaacttatta
     6541 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     6601 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     6661 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
     6721 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     6781 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     6841 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     6901 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     6961 gcaggatata ttgtggtgta aacataacgg atccggtctc aggaggtcaa catggtggag
     7021 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     7081 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     7141 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     7201 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     7261 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     7321 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     7381 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
     7441 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     7501 tttacaatta tcgatacaat ggtattcaca ctcgaagatt tcgttgggga ctggcgacag
     7561 acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag
     7621 aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg
     7681 aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag
     7741 atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg
     7801 cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg
     7861 ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg
     7921 aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga
     7981 gtaaccatca acggagtgac cggctggcgg ctgtgcgaac gcattctggc ttcggattat
     8041 aaggaccatg acggagacta taaggaccat gacctcgacg ctgcagcagc ggattataag
     8101 gacgatgacg ataagtgagc ttctctagct agagtcgatc gacaagctcg agtttctcca
     8161 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
     8221 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     8281 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgct gtcaggaggt
     8341 caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga
     8401 agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt
     8461 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
     8521 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg
     8581 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac
     8641 gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
     8701 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     8761 acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa
     8821 cattacaatt acatttacaa ttatcgatac aatggaggac gccaaaaaca taaagaaagg
     8881 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     8941 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     9001 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     9061 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     9121 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     9181 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     9241 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     9301 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     9361 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     9421 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     9481 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     9541 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     9601 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     9661 agaactgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     9721 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     9781 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     9841 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     9901 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     9961 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
    10021 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
    10081 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaggacga
    10141 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
    10201 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc
    10261 aggtctgccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
    10321 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
    10381 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
    10441 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
    10501 g
//
