LOCUS       sequence-36839-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3448 (=HL 3425 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(111..129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        197..301
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(520..539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagttcttct cctttacgca ttttttttcc tccttagtcg acagtatctc tatcactgat
       61 agggatgtca atctctatca ctgataggga ctcgaggtga agacgaaagg gcctcgtgat
      121 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      661 atgcagtgcg gccataacc
//
