LOCUS       sequence-36840-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3449 (=HL 3262 + pHL 1179)
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    240..256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        314..418
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(637..656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagttcttct cctttacgca tgctctcaca cagcgaggcc agatcgggct gctgcaatag
       61 tgtccgagtg atgtcgaaca acccttgttg tccgagatca ctcatcggtg tatatgacat
      121 tttgttcttc caggaaagca ccgcaaagcg gtgcaaaagg tcattgacta actataggag
      181 atttagaatt ttattagcat atacgcggaa gcggttcggc gtgtggtgtc gacagtatct
      241 tgttatccgc tcacaatgtc aatgttatcc gctcacattt atgacgtcag gtggcacttt
      301 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      421 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      481 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      541 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      601 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatc
//
