LOCUS       sequence-368417-        4085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Escherichia coli plasmid containing unique cloning sites behind a 
            Campylobacter jejuni cysM promoter. Cloned genes will be expressed 
            from the constitutively expressed C. jejuni promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Uhlich GA, Bagi L, Gunther NW 4th
  TITLE     Cloning vectors for gene delivery, integration and expression in 
            Campylobacter jejuni.
  JOURNAL   Biotechniques. 2022 Apr 13. doi: 10.2144/btn-2021-0096.
  PUBMED    35416085
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2022 Apr 13. doi: 10.2144/btn-2021-0096."
COMMENT     Sequence Label: pBlueKan+cysMPro
FEATURES             Location/Qualifiers
     source          1..4085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..39)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(19..37)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(58..74)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(58..74)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(71..93)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    82..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    151..172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(289..306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(460..1048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(540..559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1219..2010,2011..2079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1842..1861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2080..2184)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2210..2665)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2347..2368)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2559..2578
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2792..2814
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2806..2823
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2807..2823
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2833..2852
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2833..2851
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2965..3759
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
       61 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      121 gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      181 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      241 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      301 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      361 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      421 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      481 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      541 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      601 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      661 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      721 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      781 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      841 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      901 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      961 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1021 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1081 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1141 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1201 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1261 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1321 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1381 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1441 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1501 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1561 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1621 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1681 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1741 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1801 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1861 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1921 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1981 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2041 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2101 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2161 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     2221 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     2281 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     2341 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     2401 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     2461 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     2521 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     2581 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     2641 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     2701 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     2761 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     2821 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg agctcgcggc cgcgtttgta
     2881 gatatttgta tctatttata ggattttatg ggtttgtagg aacggtatct ttaaatactg
     2941 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
     3001 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     3061 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     3121 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     3181 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     3241 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     3301 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     3361 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
     3421 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
     3481 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     3541 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     3601 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     3661 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     3721 aaaataaaat attatatttt actggatgaa ttgttttagt accccatcag ttttattggt
     3781 tttggtactt tttcaactca agaaagagct gctagagaag ctagagtacc aagcacagga
     3841 aaaacaatca aagttcctgc tacaagagtt gcaaaattta aggtaggtaa aaaccttaaa
     3901 gaagctgttg caaaagcaag cggcaaaaag aaaaaataag acttcggcta gataaattct
     3961 agccttcttt cttaattaat tcagatattt tgtatctttt atttacttta tttttttaaa
     4021 cttttataaa actatcatta ttaaacaaaa aaggatatta aaattcccgg gctcgaggcg
     4081 gccgc
//
