LOCUS       sequence-368448-        6762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CYP110D1 coding sequence under the control of PpsbA2* promoter and 
            the BBa_B0032 RBS, for the expression in Synechocystis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Mascia F, Pereira SB, Pacheco CC, Oliveira P, Solarczek J, Schallmey
            A, Kourist R, Alphand V, Tamagnini P.
  TITLE     Light-driven hydroxylation of testosterone by Synechocystis sp. PCC 
            6803 expressing the heterologous CYP450 monooxygenase CYP110D1
  JOURNAL   Green Chemistry, 24 (2022):6156
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Green 
            Chemistry, 24 (2022):6156"
COMMENT     Sequence Label: pSEVA251::PpsbA2*::B0032::CYP110D1
FEATURES             Location/Qualifiers
     source          1..6762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    121..142
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1645..1739
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1860..1879)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1871..2686
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2853..2961
                     /note="incP origin of transfer"
     CDS             complement(2972..3823)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3810..4649)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDXXXAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5160..6131)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6238..6632
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6670..6756)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccgggcta attagggggt gtcgcccttt acacgtactt agtcgctgaa
      121 gaattcgcgg ccgcttctag agagctttac aaaactctca ttaatccttt agactaagtt
      181 tagtcagttc caatctgtac tagtcacaca ggaaagtact agatgacagt cactcaaaac
      241 ctacccaacg gccccagaat accccggttg ttacggttgt ttaagtttat tactcaacca
      301 atacagtatg tggaagattt tgccaaagtc tacggtgaca acttcactat ctgggggagt
      361 ggagaaagtt attttgtcta ctttagccat ccccaagcat tggaacagat ttttactaat
      421 gttagttgtt ttgagagtag tggtggaggt agtccgcttt tagaactttt attgggtaag
      481 aattctttaa ttttactaga aggcgatcgc catcagcgcc aacgtcaact attaacccca
      541 cctttccacg gtgaaaggat gcgggcttat ggtcaaacta ttcgtgaaat aactcaacaa
      601 gtcactcaag catggcaaat gggtaagccc ttcaatatcc gggcttccat gcaggaaatt
      661 accatgcgtg tcattttgcg ggtagtgttt ggtgttgatg agggggaatt gtttcaagaa
      721 ctgcggcaat tgctaactac cctgttagat ttcatgggtt ctcctctaat gtccagcacc
      781 ttctttttta gtttcacgca aaaggactat ggtgcatgga gtccttgggg gcgaatggtg
      841 cgcctcatca aaaaaattga ccaactgatt tacgctttga ttgctcaacg tcgggctgag
      901 tttggcgaaa atcgtcaaga tatccttagt ttattaattt cggcacgtta tgacgacgga
      961 cagccaatgt cagacgtaga gttacgggat gaattaatga caatgttggt tgcaggacat
     1021 gaaaccactg cttcagcctt gacttgggcg ttttattgga ttgatagcgt accagaagta
     1081 cgtgaaaagt tatttcagga attagataca ctgaatgacg attcagaacc cagtattatt
     1141 gccaaattgc cttatttaac agcagtttgc caagaaaccc tacgctttta tcctattgtg
     1201 ctgaatgctt tcttccggcg aacaaaaaac cctatggaaa ttatgggcta caaactgccg
     1261 aaagcaacat tagttgttcc tagtatttat ttagcccatc atcgagaaga agtttatccg
     1321 caatccaagc aattccgacc agaacgcttt ttagaaaagc aattctcccc ttacgaatac
     1381 ttaccctttg gcggcggtaa tcggcgctgc atcggtctag catttgccca atatgaaatg
     1441 aaaattgtct tagcaacaat tttgtcacaa ttccaagtat ctagactcag taagcgtccg
     1501 gtgcagcctg tgcgtcgggg attaactttg gcagcaccag gaggaatgaa gatggtggct
     1561 aataaaagaa tgcgtaattc gtaactgcag gcatgcaagc ttgcggccgc gtcgtgactg
     1621 ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg acctcagaac
     1681 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
     1741 caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat gttacattgc
     1801 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     1861 aaggggtgtt atgagccata ttcagcgtga aacgagctgt agccgtccgc gtctgaacag
     1921 caacatggat gcggatctgt atggctataa atgggcgcgt gataacgtgg gtcagagcgg
     1981 cgcgaccatt tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc tgaaacatgg
     2041 caaaggcagc gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact ggctgaccga
     2101 atttatgccg ctgccgacca ttaaacattt tattcgcacc ccggatgatg cgtggctgct
     2161 gaccaccgcg attccgggca aaaccgcgtt tcaggtgctg gaagaatatc cggatagcgg
     2221 cgaaaacatt gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca ttccggtgtg
     2281 caactgcccg tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga gccgtatgaa
     2341 caacggcctg gtggatgcga gcgattttga tgatgaacgt aacggctggc cggtggaaca
     2401 ggtgtggaaa gaaatgcata aactgctgcc gtttagcccg gatagcgtgg tgacccacgg
     2461 cgattttagc ctggataacc tgattttcga tgaaggcaaa ctgattggct gcattgatgt
     2521 gggccgtgtg ggcattgcgg atcgttatca ggatctggcc attctgtgga actgcctggg
     2581 cgaatttagc ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg ataatccgga
     2641 tatgaacaaa ctgcaatttc atctgatgct ggatgaattt ttctaataat taattggacc
     2701 gcggtccgcg cgttgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt
     2761 ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta
     2821 gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc
     2881 gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat
     2941 cctgctctgc gaggctggcc gtaggccggc ctcagcctgc cgccttgggc cgggtgatgt
     3001 cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg
     3061 cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga
     3121 catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac
     3181 acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc
     3241 tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca
     3301 cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc
     3361 gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta
     3421 tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat
     3481 agctaccttt gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca
     3541 gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc
     3601 cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc
     3661 cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct
     3721 tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt
     3781 gccggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc
     3841 ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac
     3901 cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg
     3961 cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag
     4021 gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg
     4081 cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg
     4141 gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt
     4201 tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg
     4261 gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc
     4321 ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca
     4381 agggcgtgca ggcggtgatg aatggcggnn nnnnggtctt cggcgggcag gtagatcacc
     4441 gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc
     4501 agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta
     4561 ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc
     4621 agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt
     4681 aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc
     4741 gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct
     4801 tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg
     4861 tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc
     4921 ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg
     4981 actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa
     5041 tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata
     5101 aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac
     5161 tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt
     5221 gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc
     5281 tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc
     5341 atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata
     5401 aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg
     5461 tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg
     5521 gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc
     5581 tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac
     5641 ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg
     5701 ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg
     5761 gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag
     5821 ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc
     5881 aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc
     5941 ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc
     6001 acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg
     6061 atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg
     6121 tcgttcttca tcgggccacc aagcgattcc cacacattat acgagccgga agcataaagt
     6181 gtagctagat ccgaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc
     6241 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct
     6301 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct
     6361 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct
     6421 ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct
     6481 agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc
     6541 ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg
     6601 caggggggcg tgacagttat tgcaggggtt cgtgacagtt agggcgcgcc cagctgtcta
     6661 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     6721 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ct
//
