LOCUS       sequence-368484-        9823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FMRP fused to HaloTag for protein localization through 
            fluorescence imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Monday HR, Kharod SC, Yoon YJ, Singer RH, Castillo PE
  TITLE     Presynaptic FMRP and local protein synthesis support structural and 
            functional plasticity of glutamatergic axon terminals.
  JOURNAL   Neuron. 2022 Aug 17;110(16):2588-2606.e6. doi: 
            10.1016/j.neuron.2022.05.024. Epub 2022 Jun 20.
  PUBMED    35728596
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2022.05.024"; journalName: "Neuron"; date: 
            "2022-08-17- 17"; volume: "110"; issue: "16"; pages: "2588-2606.e6"
COMMENT     Sequence Label: p323.hSyn.Halo-mFMRP.Wpre
FEATURES             Location/Qualifiers
     source          1..9823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(80..148,149..940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(298..317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1111..1699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1600..1619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1853..1870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1987..2008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2023..2028,2029..2046,2047..2053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2061..2077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2066..2088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2085..2101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2085..2101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2120..2140
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2122..2140
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2241..2620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2621..2824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2775..2795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2839..3019
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3066..3191
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3684..3917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4102..4146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4413..4530
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4555..5002
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      5026..5035
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5032..5922
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             5965..5988
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7834..8422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7887..7907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8305..8316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8509..8742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8814..8935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8851..8870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8905..8924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8975..9110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9130..9149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9131..9149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9159..9176)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9159..9175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9168..9190)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9317..9772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9404..9423)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9614..9635
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(9798..9823,1..79)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
       61 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      121 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      181 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      241 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      301 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      361 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      421 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      481 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      541 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      601 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      661 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      721 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      781 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      841 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
      901 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
      961 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1021 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1441 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1501 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1561 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1621 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1681 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1741 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1801 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1861 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1921 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1981 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2041 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2101 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     2161 agcttggcca ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg
     2221 tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     2281 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2341 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2401 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2461 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2521 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2581 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2641 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2701 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2761 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2821 agctcgttta gtgaaccggg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2881 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2941 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3001 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     3061 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     3121 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     3181 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     3241 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     3301 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     3361 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     3421 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     3481 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     3541 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     3601 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     3661 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     3721 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     3781 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     3841 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     3901 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     3961 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     4021 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     4081 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     4141 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     4201 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     4261 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     4321 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     4381 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
     4441 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4501 caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagagtgca
     4561 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
     4621 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
     4681 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
     4741 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
     4801 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
     4861 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
     4921 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
     4981 cgtgtcgtgc ctgagagcgc agctgcaggt cgagatccac cggtcgccac catggcagaa
     5041 atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac
     5101 tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc
     5161 tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct
     5221 ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac
     5281 cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc
     5341 attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc
     5401 aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa
     5461 tttgctcgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc
     5521 gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa
     5581 gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg
     5641 cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa
     5701 gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca
     5761 ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag
     5821 gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc
     5881 agcgagatcg cgcgctggct gtctactctg gagatttccg gttccggact cagatctcga
     5941 gctcaagctt cgaattcgat ggaggactac aaggacgacg atgacaagga gctggtggtg
     6001 gaagtgcggg gctccaatgg cgctttctac aaggcatttg taaaagatgt ccatgaagat
     6061 tctataacag ttgcttttga aaacaactgg caaccagaga gacagattcc attccatgat
     6121 gtgagattcc caccacctgt aggttataat aaagatataa acgaaagtga tgaagttgag
     6181 gtttattcca gagcaaatga aaaagagcct tgctgttggt ggttagctaa agtgaggatg
     6241 ataaagggtg agttttatgt gatagaatat gcagcatgtg atgctacgta taatgaaatt
     6301 gtcacaattg agcgtctacg atctgttaat cccaacaaac ctgctacaaa agatactttc
     6361 cataagatca agctggaggt gccagaagat ttacgacaaa tgtgtgccaa agaatcagca
     6421 cataaggatt ttaaaaaggc agttggtgcc ttctctgtaa cttatgatcc agaaaattat
     6481 cagctggtaa ttttgtccat caatgaagtc acctcaaagc gagcccacat gttgattgac
     6541 atgcactttc gaagtctgcg caccaagttg tctcttatac tgagaaatga agaagccagt
     6601 aaacaactgg agagttcaag gcagcttgcc tcaagatttc atgaacagtt tatcgtacga
     6661 gaagatctga tgggtttagc tattggtact catggtgcta atattcagca agctagaaaa
     6721 gtgcctggcg tcactgctat tgatttagat gaggatacct gcacatttca tatttatgga
     6781 gaggatcaag atgcagtgaa aaaggctaga agctttctgg aatttgctga agatgtcata
     6841 caggttccac gaaacttagt aggcaaagta ataggaaaaa atggaaagct gattcaagag
     6901 atcgtggaca agtcaggagt tgtgagggtg aggattgagg ctgaaaatga gaaaagtgtc
     6961 ccacaagaag aggaaattat gccaccaagt tccctacctt ccaataattc aagggttgga
     7021 cctaactcct ctgaagaaaa gaaacattta gatacaaagg aaaacaccca tttttctcaa
     7081 cctaacagta caaaagtcca gagggtgtta gtggtttcat caattgtagc agggggaccc
     7141 cagaaacctg aacccaaggc ttggcagggt atggtaccat ttgtttttgt gggaacaaaa
     7201 gacagcatcg ctaatgccac tgttcttttg gattatcacc tgaactattt aaaggaagta
     7261 gaccagttgc gtttggagag attacaaatt gatgagcagt tgcgacaaat tggagctagt
     7321 tctagaccac caccaaatcg tacagataag gaaaaaggct atgtgactga tgatggtcaa
     7381 ggaatgggtc gaggtagtag accttacaga aatagggggc acggcagacg cggtcctgga
     7441 tatacttcag gaactaattc tgaagcatca aatgcttctg aaacagaatc tgaccacaga
     7501 gacgaactca gtgattggtc attagctcca acagaggaag agagggagag cttcctgcgc
     7561 agaggagacg gacggcggcg tggaggagga ggaagaggac aaggaggaag aggaagagga
     7621 ggaggcttca aaggaaacga cgatcattcc cgaacagata atcgtccacg taatccaaga
     7681 gaggctaaag gaagaacagc tgatggatcc ctgcagagtg cctccagtga agggagccgg
     7741 ctgcgcacgg gtaaagatcg taaccagaag aaggaaaagc cagacagcgt agatgggctg
     7801 caaccgctgg tgaatggagt accctaagtc gacaatcaac ctctggatta caaaatttgt
     7861 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7921 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7981 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8041 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8101 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8161 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8221 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     8281 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8341 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8401 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt
     8461 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8521 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8761 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8941 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9061 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     9121 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     9181 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     9241 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     9301 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9361 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9421 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9481 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9541 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9601 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9661 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9721 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     9781 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt ttt
//
