LOCUS       sequence-368552-        9296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1, position 2 reverse, transcriptional unit for 
            expression of plant codon optimized Cas9 from Streptococcus pyogenes
            driven by 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD1CB0001
FEATURES             Location/Qualifiers
     source          1..9296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    156..180
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        303..648
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     591..611
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             739..4842
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             739..4842
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4855..4875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4891..5143
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5254..5278
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      5370..6001
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6088..6192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6193..6261,6262..7053)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6411..6430)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7224..7812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7713..7732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8148..9296
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgcctgatc tggggaaccc
       61 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
      121 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
      181 aacactgata gtttaaacca cttcgtgcag aagacaatag tggaggtcaa catggtggag
      241 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
      301 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
      361 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
      421 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
      481 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
      541 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      601 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
      661 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
      721 tttacaatta tcgatacaat ggataagaag tactctatcg gactcgatat cggaactaac
      781 tctgtgggat gggctgtgat caccgatgag tacaaggtgc catctaagaa gttcaaggtt
      841 ctcggaaaca ccgataggca ctctatcaag aaaaacctta tcggtgctct cctcttcgat
      901 tctggtgaaa ctgctgaggc taccagactc aagagaaccg ctagaagaag gtacaccaga
      961 agaaagaaca ggatctgcta cctccaagag atcttctcta acgagatggc taaagtggat
     1021 gattcattct tccacaggct cgaagagtca ttcctcgtgg aagaagataa gaagcacgag
     1081 aggcacccta tcttcggaaa catcgttgat gaggtggcat accacgagaa gtaccctact
     1141 atctaccacc tcagaaagaa gctcgttgat tctactgata aggctgatct caggctcatc
     1201 tacctcgctc tcgctcacat gatcaagttc agaggacact tcctcatcga gggtgatctc
     1261 aaccctgata actctgatgt ggataagttg ttcatccagc tcgtgcagac ctacaaccag
     1321 cttttcgaag agaaccctat caacgcttca ggtgtggatg ctaaggctat cctctctgct
     1381 aggctctcta agtcaagaag gcttgagaac ctcattgctc agctccctgg tgagaagaag
     1441 aacggacttt tcggaaactt gatcgctctc tctctcggac tcacccctaa cttcaagtct
     1501 aacttcgatc tcgctgagga tgcaaagctc cagctctcaa aggataccta cgatgatgat
     1561 ctcgataacc tcctcgctca gatcggagat cagtacgctg atttgttcct cgctgctaag
     1621 aacctctctg atgctatcct cctcagtgat atcctcagag tgaacaccga gatcaccaag
     1681 gctccactct cagcttctat gatcaagaga tacgatgagc accaccagga tctcacactt
     1741 ctcaaggctc ttgttagaca gcagctccca gagaagtaca aagagatttt cttcgatcag
     1801 tctaagaacg gatacgctgg ttacatcgat ggtggtgcat ctcaagaaga gttctacaag
     1861 ttcatcaagc ctatcctcga gaagatggat ggaaccgagg aactcctcgt gaagctcaat
     1921 agagaggatc ttctcagaaa gcagaggacc ttcgataacg gatctatccc tcatcagatc
     1981 cacctcggag agttgcacgc tatccttaga aggcaagagg atttctaccc attcctcaag
     2041 gataacaggg aaaagattga gaagattctc accttcagaa tcccttacta cgtgggacct
     2101 ctcgctagag gaaactcaag attcgcttgg atgaccagaa agtctgagga aaccatcacc
     2161 ccttggaact tcgaagaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg
     2221 atgaccaact tcgataagaa ccttccaaac gagaaggtgc tccctaagca ctctttgctc
     2281 tacgagtact tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg
     2341 aggaagcctg cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag
     2401 accaacagaa aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc
     2461 ttcgattcag ttgagatttc tggtgttgag gataggttca acgcatctct cggaacctac
     2521 cacgatctcc tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat
     2581 atcttggagg atatcgttct taccctcacc ctctttgaag atagagagat gattgaagaa
     2641 aggctcaaga cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga
     2701 agatacactg gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag
     2761 tctggaaaga caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg
     2821 cagctcatcc acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca
     2881 ggacagggtg atagtctcca tgagcatatc gctaacctcg ctggatctcc tgcaatcaag
     2941 aagggaatcc tccagactgt gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat
     3001 aagcctgaga acatcgtgat cgaaatggct agagagaacc agaccactca gaagggacag
     3061 aagaactcta gggaaaggat gaagaggatc gaggaaggta tcaaagagct tggatctcag
     3121 atcctcaaag agcaccctgt tgagaacact cagctccaga atgagaagct ctacctctac
     3181 tacctccaga acggaaggga tatgtatgtg gatcaagagt tggatatcaa caggctctct
     3241 gattacgatg ttgatcatat cgtgccacag tcattcttga aggatgattc tatcgataac
     3301 aaggtgctca ccaggtctga taagaacagg ggtaagagtg ataacgtgcc aagtgaagag
     3361 gttgtgaaga aaatgaagaa ctattggagg cagctcctca acgctaagct catcactcag
     3421 agaaagttcg ataacttgac taaggctgag aggggaggac tctctgaatt ggataaggca
     3481 ggattcatca agaggcagct tgtggaaacc aggcagatca ctaagcacgt tgcacagatc
     3541 ctcgattcta ggatgaacac caagtacgat gagaacgata agttgatcag ggaagtgaag
     3601 gttatcaccc tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca attctacaag
     3661 gtgagggaaa tcaacaacta ccaccacgct cacgatgctt accttaacgc tgttgttgga
     3721 accgctctca tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg tgattacaag
     3781 gtgtacgatg tgaggaagat gatcgctaag tctgagcaag agatcggaaa ggctaccgct
     3841 aagtatttct tctactctaa catcatgaat ttcttcaaga ccgagattac cctcgctaac
     3901 ggtgagatca gaaagaggcc actcatcgag acaaacggtg aaacaggtga gatcgtgtgg
     3961 gataagggaa gggatttcgc taccgttaga aaggtgctct ctatgccaca ggtgaacatc
     4021 gttaagaaaa ccgaggtgca gaccggtgga ttctctaaag agtctatcct ccctaagagg
     4081 aactctgata agctcattgc taggaagaag gattgggacc ctaagaaata cggtggtttc
     4141 gattctccta ccgtggctta ctctgttctc gttgtggcta aggttgagaa gggaaagagt
     4201 aagaagctca agtctgttaa ggaacttctc ggaatcacta tcatggaaag gtcatctttc
     4261 gagaagaacc caatcgattt cctcgaggct aagggataca aagaggttaa gaaggatctc
     4321 atcatcaagc tcccaaagta ctcactcttc gaactcgaga acggtagaaa gaggatgctc
     4381 gcttctgctg gtgagcttca aaagggaaac gagcttgctc tcccatctaa gtacgttaac
     4441 tttctttacc tcgcttctca ctacgagaag ttgaagggat ctccagaaga taacgagcag
     4501 aagcaacttt tcgttgagca gcacaagcac tacttggatg agatcatcga gcagatctct
     4561 gagttctcta aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac
     4621 aacaagcaca gagataagcc tatcagggaa caggcagaga acatcatcca tctcttcacc
     4681 cttaccaacc tcggtgctcc tgctgctttc aagtacttcg atacaaccat cgataggaag
     4741 agatacacct ctaccaaaga agtgctcgat gctaccctca tccatcagtc tatcactgga
     4801 ctctacgaga ctaggatcga tctctcacag ctcggtggtg attcaagggc tgatcctaag
     4861 aagaagagga aggtttgagc ttgtcaagca gatcgttcaa acatttggca ataaagtttc
     4921 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     4981 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     5041 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     5101 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgacgcta agcttgaatt
     5161 cttgcttgtc ttcacagagt ggggcccact gcatccaccc cagtacatta aaaacgtccg
     5221 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     5281 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     5341 cccatcagtc agatcaggat ctcctttgcg acgctcaccg ggctggttgc cctcgccgct
     5401 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
     5461 agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     5521 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     5581 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     5641 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     5701 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     5761 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     5821 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     5881 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
     5941 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
     6001 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
     6061 aaaaatggca gcgctggcca attcgtgcgc ggaaccccta tttgtttatt tttctaaata
     6121 cattcaaata tgtatccgct catgagacaa taaccttgat aaatgcttca ataatattga
     6181 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6241 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6301 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6361 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6421 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6481 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6541 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6601 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6661 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6721 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6781 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6841 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6901 gagcgtggtt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6961 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7021 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7081 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7141 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcaga tcctagatgt
     7861 ggcgcaacga tgccggcgac aagcaggagc gcaccgactt cttccgcatc aagtgttttg
     7921 gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg gtcgctggta ttcgtgcagg
     7981 gcaagattcg gaataccaag tacgagaagg acggccagac ggtctacggg accgacttca
     8041 ttgccgataa ggtggattat ctggacacca aggcaccagg cgggtcaaat caggaataag
     8101 ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg agggtgaatg aatcggacgt
     8161 ttgaccggaa ggcatacagg caagaactga tcgacgcggg gttttccgcc gaggatgccg
     8221 aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga aaccttccag tccgtcggct
     8281 cgatggtcca gcaagctacg gccaagatcg agcgcgacag cgtgcaactg gctccccctg
     8341 ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg tcttgaacag gaggcggcag
     8401 gtttggcgaa gtcgatgacc atcgacacgc gaggaactat gacgaccaag aagcgaaaaa
     8461 ccgccggcga ggacctggca aaacaggtca gcgaggccaa gcaggccgcg ttgctgaaac
     8521 acacgaagca gcagatcaag gaaatgcagc tttccttgtt cgatattgcg ccgtggccgg
     8581 acacgatgcg agcgatgcca aacgacacgg cccgctctgc cctgttcacc acgcgcaaca
     8641 agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt ccacgtcaac aaggacgtga
     8701 agatcaccta caccggcgtc gagctgcggg ccgacgatga cgaactggtg tggcagcagg
     8761 tgttggagta cgcgaagcgc acccctatcg gcgagccgat caccttcacg ttctacgagc
     8821 tttgccagga cctgggctgg tcgatcaatg gccggtatta cacgaaggcc gaggaatgcc
     8881 tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga ccgcgttggg cacctggaat
     8941 cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg caagaaaacg tcccgttgcc
     9001 aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg cgaccactac acgaaattca
     9061 tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg gatgttcgac tatttcagct
     9121 cgcaccggga gccgtacccg ctcaagctgg aaaccttccg cctcatgtgc ggatcggatt
     9181 ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc ctgcgaagag ttgcgaggca
     9241 gcggcctggt ggaacacgcc tgggtcaatg atgacctggt gcattgcaaa cgctag
//
