LOCUS       sequence-368553-        9296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1, position 5 reverse, transcriptional unit for 
            expression of plant codon optimized Cas9 from Streptococcus pyogenes
            driven by 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD1CB0202
FEATURES             Location/Qualifiers
     source          1..9296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(218..1009,1010..1078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     841..860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1079..1183)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1270..1901
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    1993..2017
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(2128..2380)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2396..2416)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2429..6532)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2429..6532)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(6623..6968)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6660..6680)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7091..7115
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7271..8419)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(join(8755..9296,1..47))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8835..8854)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
       61 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
      241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
      301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
      361 aaccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
      421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     1081 tcttcctttt tcaatattat tgaagcattt atcaaggtta ttgtctcatg agcggataca
     1141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacgaat tggccagcgc
     1201 tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag
     1261 gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
     1321 gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag
     1381 caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg
     1441 attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac
     1501 tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt
     1561 gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt
     1621 aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc
     1681 gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt
     1741 ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc
     1801 cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg
     1861 ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cgcaaaggag atcctgatct
     1921 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     1981 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
     2041 taacacattg cggacgtttt taatgtactg gggtggatgc agtgggcccc actctgtgaa
     2101 gacaacagag aattcgcatg cagcgtcgat ctagtaacat agatgacacc gcgcgcgata
     2161 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
     2221 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
     2281 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat
     2341 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgacaa gctcaaacct
     2401 tcctcttctt cttaggatca gcccttgaat caccaccgag ctgtgagaga tcgatcctag
     2461 tctcgtagag tccagtgata gactgatgga tgagggtagc atcgagcact tctttggtag
     2521 aggtgtatct cttcctatcg atggttgtat cgaagtactt gaaagcagca ggagcaccga
     2581 ggttggtaag ggtgaagaga tggatgatgt tctctgcctg ttccctgata ggcttatctc
     2641 tgtgcttgtt gtaagcagac aacaccttat cgaggtttgc atcagcgagg atcacccttt
     2701 tagagaactc agagatctgc tcgatgatct catccaagta gtgcttgtgc tgctcaacga
     2761 aaagttgctt ctgctcgtta tcttctggag atcccttcaa cttctcgtag tgagaagcga
     2821 ggtaaagaaa gttaacgtac ttagatggga gagcaagctc gtttcccttt tgaagctcac
     2881 cagcagaagc gagcatcctc tttctaccgt tctcgagttc gaagagtgag tactttggga
     2941 gcttgatgat gagatccttc ttaacctctt tgtatccctt agcctcgagg aaatcgattg
     3001 ggttcttctc gaaagatgac ctttccatga tagtgattcc gagaagttcc ttaacagact
     3061 tgagcttctt actctttccc ttctcaacct tagccacaac gagaacagag taagccacgg
     3121 taggagaatc gaaaccaccg tatttcttag ggtcccaatc cttcttccta gcaatgagct
     3181 tatcagagtt cctcttaggg aggatagact ctttagagaa tccaccggtc tgcacctcgg
     3241 ttttcttaac gatgttcacc tgtggcatag agagcacctt tctaacggta gcgaaatccc
     3301 ttcccttatc ccacacgatc tcacctgttt caccgtttgt ctcgatgagt ggcctctttc
     3361 tgatctcacc gttagcgagg gtaatctcgg tcttgaagaa attcatgatg ttagagtaga
     3421 agaaatactt agcggtagcc tttccgatct cttgctcaga cttagcgatc atcttcctca
     3481 catcgtacac cttgtaatca ccgtacacga actctgactc gagcttagga tacttcttga
     3541 tgagagcggt tccaacaaca gcgttaaggt aagcatcgtg agcgtggtgg tagttgttga
     3601 tttccctcac cttgtagaat tggaaatcct ttctgaaatc agacacgagc tttgacttga
     3661 gggtgataac cttcacttcc ctgatcaact tatcgttctc atcgtacttg gtgttcatcc
     3721 tagaatcgag gatctgtgca acgtgcttag tgatctgcct ggtttccaca agctgcctct
     3781 tgatgaatcc tgccttatcc aattcagaga gtcctcccct ctcagcctta gtcaagttat
     3841 cgaactttct ctgagtgatg agcttagcgt tgaggagctg cctccaatag ttcttcattt
     3901 tcttcacaac ctcttcactt ggcacgttat cactcttacc cctgttctta tcagacctgg
     3961 tgagcacctt gttatcgata gaatcatcct tcaagaatga ctgtggcacg atatgatcaa
     4021 catcgtaatc agagagcctg ttgatatcca actcttgatc cacatacata tcccttccgt
     4081 tctggaggta gtagaggtag agcttctcat tctggagctg agtgttctca acagggtgct
     4141 ctttgaggat ctgagatcca agctctttga taccttcctc gatcctcttc atcctttccc
     4201 tagagttctt ctgtcccttc tgagtggtct ggttctctct agccatttcg atcacgatgt
     4261 tctcaggctt atgccttccc atcaccttca ccaactcatc cacaaccttc acagtctgga
     4321 ggattccctt cttgattgca ggagatccag cgaggttagc gatatgctca tggagactat
     4381 caccctgtcc tgaaacctga gccttctgga tatcctcttt aaaggtgaga gaatcatcgt
     4441 ggatgagctg catgaagttt ctgttagcga atccatcaga cttgaggaaa tcaaggattg
     4501 tctttccaga ctgcttatcc ctgattccgt taatgagctt tcttgagagc cttccccaac
     4561 cagtgtatct tcttctcttc aactgcttca tcaccttatc atcgaagaga tgagcgtagg
     4621 tcttgagcct ttcttcaatc atctctctat cttcaaagag ggtgagggta agaacgatat
     4681 cctccaagat atcctcgttt tcctcgttat ccaagaaatc cttatcctta atgatcttga
     4741 ggagatcgtg gtaggttccg agagatgcgt tgaacctatc ctcaacacca gaaatctcaa
     4801 ctgaatcgaa gcactcgatt ttcttgaagt aatcctcttt gagctgcttc acggtcacct
     4861 ttctgttggt cttgaacaag agatcaacga tagccttctt ttgctcacct gacaaaaaag
     4921 caggcttcct cattccctcg gtcacgtact taaccttggt caactcgttg tacacggtga
     4981 agtactcgta gagcaaagag tgcttaggga gcaccttctc gtttggaagg ttcttatcga
     5041 agttggtcat cctctcgatg aaagactgag cactagcacc cttatccacc acctcttcga
     5101 agttccaagg ggtgatggtt tcctcagact ttctggtcat ccaagcgaat cttgagtttc
     5161 ctctagcgag aggtcccacg tagtaaggga ttctgaaggt gagaatcttc tcaatctttt
     5221 ccctgttatc cttgaggaat gggtagaaat cctcttgcct tctaaggata gcgtgcaact
     5281 ctccgaggtg gatctgatga gggatagatc cgttatcgaa ggtcctctgc tttctgagaa
     5341 gatcctctct attgagcttc acgaggagtt cctcggttcc atccatcttc tcgaggatag
     5401 gcttgatgaa cttgtagaac tcttcttgag atgcaccacc atcgatgtaa ccagcgtatc
     5461 cgttcttaga ctgatcgaag aaaatctctt tgtacttctc tgggagctgc tgtctaacaa
     5521 gagccttgag aagtgtgaga tcctggtggt gctcatcgta tctcttgatc atagaagctg
     5581 agagtggagc cttggtgatc tcggtgttca ctctgaggat atcactgagg aggatagcat
     5641 cagagaggtt cttagcagcg aggaacaaat cagcgtactg atctccgatc tgagcgagga
     5701 ggttatcgag atcatcatcg taggtatcct ttgagagctg gagctttgca tcctcagcga
     5761 gatcgaagtt agacttgaag ttaggggtga gtccgagaga gagagcgatc aagtttccga
     5821 aaagtccgtt cttcttctca ccagggagct gagcaatgag gttctcaagc cttcttgact
     5881 tagagagcct agcagagagg atagccttag catccacacc tgaagcgttg atagggttct
     5941 cttcgaaaag ctggttgtag gtctgcacga gctggatgaa caacttatcc acatcagagt
     6001 tatcagggtt gagatcaccc tcgatgagga agtgtcctct gaacttgatc atgtgagcga
     6061 gagcgaggta gatgagcctg agatcagcct tatcagtaga atcaacgagc ttctttctga
     6121 ggtggtagat agtagggtac ttctcgtggt atgccacctc atcaacgatg tttccgaaga
     6181 tagggtgcct ctcgtgcttc ttatcttctt ccacgaggaa tgactcttcg agcctgtgga
     6241 agaatgaatc atccacttta gccatctcgt tagagaagat ctcttggagg tagcagatcc
     6301 tgttctttct tctggtgtac cttcttctag cggttctctt gagtctggta gcctcagcag
     6361 tttcaccaga atcgaagagg agagcaccga taaggttttt cttgatagag tgcctatcgg
     6421 tgtttccgag aaccttgaac ttcttagatg gcaccttgta ctcatcggtg atcacagccc
     6481 atcccacaga gttagttccg atatcgagtc cgatagagta cttcttatcc attgtatcga
     6541 taattgtaaa tgtaattgta atgttgtttg ttgtttgttg ttgttggtaa ttgttgtaaa
     6601 aatgagctct tatactcgag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     6661 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatgtcac
     6721 atcaatccac ttgctttgta gacgtggttg gaacctcttc tttttccacg atgctcctcg
     6781 tgggtggggg tccatctttg ggaccactgt cggcagagag atcttgaatg atagcctttc
     6841 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttctactgtc ctttcgatga
     6901 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgaaattatc ctttgttgaa
     6961 aagtctcaat agccctttga tcttctgaga ctgtatcttt gacatttttg gagtagacca
     7021 gagtgtcgtg ctccaccatg ttgacctcct gtgttgtctt ctgcacgaag tggtttaaac
     7081 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     7141 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     7201 gccaaccaca gggttcccca gatcaggcgc tggctgctga acccccagcc ggaactgacc
     7261 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     7321 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     7381 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     7441 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     7501 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     7561 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     7621 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     7681 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     7741 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     7801 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     7861 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     7921 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     7981 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     8041 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     8101 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     8161 ccaaacctgc cgcctcctgt tcaagacgac gcgaacgctc cacggcggcc gatggcgcgg
     8221 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     8281 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     8341 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     8401 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     8461 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     8521 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     8581 tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct
     8641 gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt
     8701 tgcgccacat ctaggatctg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8761 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8821 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8881 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8941 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9001 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9061 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9121 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9181 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9241 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtt
//
