LOCUS       sequence-368554-       11489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified tobacco rattle virus RNA2 acceptor vector that could be 
            used for expression of sgRNAs, contains restriction enzyme sites for
            golden gate assembly using AarI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0KN0750
FEATURES             Location/Qualifiers
     source          1..11489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    425..449
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        978..1323
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1266..1286
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(3006..3329)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     3224..3246
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3238..3255
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3239..3255
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3256..3312
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3325..3341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3325..3341)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3338..3360)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3349..3365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3373..3379,3380..3397,3398..3403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3418..3439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             3970..3987
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4296..4320
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5621..6250
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6679..7752
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7818..8012
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8248..8270)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8356..8496
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8511..8528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8682..9270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8762..8781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10695..11489)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
       61 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
      121 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
      181 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
      241 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      301 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      361 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      421 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      481 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcctaggcca ccatgttggg
      541 cccggcgcgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac actctcgtct
      601 actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac
      661 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
      721 aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg
      781 ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga
      841 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgatg
      901 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
      961 gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     1021 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc
     1081 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt
     1141 ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc
     1201 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     1261 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     1321 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat
     1381 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc
     1441 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac
     1501 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt
     1561 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac
     1621 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg
     1681 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt
     1741 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg
     1801 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg
     1861 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg
     1921 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga
     1981 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac
     2041 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg
     2101 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag
     2161 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga
     2221 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg
     2281 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt
     2341 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact
     2401 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc
     2461 cagcgagtgg aggtccgata cgtcctaatc cctagggatt taaggacgtg aactctgttg
     2521 agatctctgt gaaattcaga gggtgggtga taccatattc actgatgcca ttagcgacat
     2581 ctaaataggg ctaattgtga ctaatttgag ggaatttcct ttaccattga cgtcagtgtc
     2641 gttggtagca tttgagtttc ggcacacaag gttaaaaacg ctgtagtaat acatgcgcaa
     2701 gaacaggctg agcatcttgt tctggggttt cacactatct ttagagaaag tgttaagtta
     2761 attaagttat cttaattaag agcataatta tactgatttg tctctcgttg atagagtcta
     2821 tcattctgtt attaaaaatt tgacaactcg gtttgctgac ctactggtca ctgtatcact
     2881 tacccgagtt aacgagtcta gaaacggcgg caggtggtca cagcttgtct gtaagcggat
     2941 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     3001 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3061 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     3121 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     3181 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     3241 aaaacgacgg ccagtgaatt cgagctcggt acccggggat cctctagagt cgacctgcag
     3301 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3361 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3421 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3481 gtcgtgccag ctacacctgc gctcagtgcc gggcatgtcc cgaagacatt aaactacggt
     3541 tctttaagta gatccgtgtc tgaagtttta ggttcaattt aaacctacga gattgacatt
     3601 ctcgactgat cttgattgat cggtaagtct tttgtaattt aattttcttt ttgattttat
     3661 tttaaattgt tatctgtttc tgtgtataga ctgtttgaga tcggcgtttg gccgactcat
     3721 tgtcttacca taggggaacg gactttgttt gtgttgttat tttatttgta ttttattaaa
     3781 attctcaacg atctgaaaaa gcctcgcggc taagagattg ttggggggtg agtaagtact
     3841 tttaaagtga tgatggttac aaaggcaaaa ggggtaaaac ccctcgccta cgtaagcgtt
     3901 attacgcccg tctgtactta tatcagtaca ctgacgagtc cctaaaggac gaaacgggag
     3961 aacgctagcc accaccacca ccaccacgtg tgaattacag gtgaccagct cgaatttccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac
     4321 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     4381 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     4441 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     4501 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     4561 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     4621 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     4681 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     4741 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     4801 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     4861 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     4921 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     4981 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     5041 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     5101 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     5161 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     5221 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     5281 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     5341 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     5401 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     5461 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     5521 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     5581 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     5641 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     5701 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     5761 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     5821 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     5881 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     5941 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     6001 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     6061 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     6121 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     6181 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     6241 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     6301 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     6361 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     6421 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     6481 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     6541 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     6601 cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc
     6661 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     6721 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     6781 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     6841 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     6901 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     6961 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     7021 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     7081 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     7141 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     7201 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     7261 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     7321 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     7381 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     7441 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     7501 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     7561 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     7621 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     7681 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     7741 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     7801 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     7861 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     7921 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     7981 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     8041 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     8101 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     8161 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     8221 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8281 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8341 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     8401 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     8461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     8521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9301 gacgctcagt ggaacgaaaa ctcacgttaa gggctgatga atcccctaat gatttttatc
     9361 aaaatcatta agttaaggta gatacacatc ttgtcatatg atcaaatggt ttcgccaaaa
     9421 atcaataatc agacaacaaa atgtgcgaac tcgatatttt acacgactct ctttaccaat
     9481 tctgccccga attacactta aaacgactca acagcttaac gttggcttgc cacgccttac
     9541 ttgactgtaa aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa
     9601 caaaacataa catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc
     9661 gactcgctgt ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg
     9721 tacttgttga ctggtctgat atccgtgagc aaaaacggct tatggtattg cgagcttcag
     9781 tcgcactaca cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat
     9841 gttcaaagaa agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca
     9901 ccacaccgct cattgtcagt gatgctggct ttaaagtgcc atggtataaa tccgttgaga
     9961 agctgggttg gtactggtta agtcgagtaa gaggaaaagt acaatatgca gacctaggag
    10021 cggaaaactg gaaacctatc agcaacttac atgatatgtc atctagtcac tcaaagactt
    10081 taggctataa gaggctgact aaaagcaatc caatctcatg ccaaattcta ttgtataaat
    10141 ctcgctctaa aggccgaaaa aatcagcgct cgacacggac tcattgtcac cacccgtcac
    10201 ctaaaatcta ctcagcgtcg gcaaaggagc catggattct agcaactaac ttacctgttg
    10261 aaattcgaac acccaaacaa cttgttaata tctattcgaa gcgaatgcag attgaagaaa
    10321 ccttccgaga cttgaaaagt cctgcctacg gactaggcct acgccatagc cgaacgagca
    10381 gctcagagcg ttttgatatc atgctgctaa tcgccctgat gcttcaacta acatgttggc
    10441 ttgcgggcgt tcatgctcag aaacaaggtt gggacaagca cttccaggct aacacagtca
    10501 gaaatcgaaa cgtactctca acagttcgct taggcatgga agttttgcgg cattctggct
    10561 acacaataac aagggaagac tcactcgtgg ctgcaaccct gcttactcaa aatctattca
    10621 cacatggtta cgttttgggg aaattatgag gggatctctc agcgttaagg gattttggtc
    10681 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
    10741 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
    10801 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
    10861 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
    10921 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
    10981 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
    11041 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
    11101 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
    11161 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
    11221 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
    11281 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
    11341 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
    11401 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
    11461 aattccggtg atattctcat tttagccat
//
