LOCUS       sequence-368556-       11427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified tobacco rattle virus RNA2 acceptor vector that could be 
            used for expression of sgRNAs, contains restriction enzyme sites for
            golden gate assembly using BsaI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0KN0751
FEATURES             Location/Qualifiers
     source          1..11427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    425..449
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        978..1323
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1266..1286
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(3001..3270)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
     primer_bind     3219..3241
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3233..3250
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3234..3250
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3266..3282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3266..3282)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3279..3301)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3290..3306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3314..3320,3321..3338,3339..3344))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3359..3380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             3908..3925
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3960..4212
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4234..4258
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5559..6188
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6617..7690
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7756..7950
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8186..8208)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8294..8434
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8449..8466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8620..9208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8700..8719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10633..11427)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
       61 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
      121 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
      181 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
      241 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      301 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      361 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      421 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      481 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcctaggcca ccatgttggg
      541 cccggcgcgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac actctcgtct
      601 actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac
      661 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
      721 aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg
      781 ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga
      841 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgatg
      901 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
      961 gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     1021 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc
     1081 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt
     1141 ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc
     1201 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     1261 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     1321 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat
     1381 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc
     1441 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac
     1501 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt
     1561 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac
     1621 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg
     1681 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt
     1741 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg
     1801 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg
     1861 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg
     1921 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga
     1981 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac
     2041 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg
     2101 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag
     2161 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga
     2221 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg
     2281 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt
     2341 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact
     2401 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc
     2461 cagcgagtgg aggtccgata cgtcctaatc cctagggatt taaggacgtg aactctgttg
     2521 agatctctgt gaaattcaga gggtgggtga taccatattc actgatgcca ttagcgacat
     2581 ctaaataggg ctaattgtga ctaatttgag ggaatttcct ttaccattga cgtcagtgtc
     2641 gttggtagca tttgagtttc ggcacacaag gttaaaaacg ctgtagtaat acatgcgcaa
     2701 gaacaggctg agcatcttgt tctggggttt cacactatct ttagagaaag tgttaagtta
     2761 attaagttat cttaattaag agcataatta tactgatttg tctctcgttg atagagtcta
     2821 tcattctgtt attaaaaatt tgacaactcg gtttgctgac ctactggtca ctgtatcact
     2881 tacccgagtt aacgagtcta gaaactgaga cctcacagct tgtctgtaag cggatgccgg
     2941 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     3001 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     3061 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     3121 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3181 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     3241 gacggccagt gagtccgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3421 tgtcgtgcca gctaggtctc aagtgcccgg gcatgtcccg aagacattaa actacggttc
     3481 tttaagtaga tccgtgtctg aagttttagg ttcaatttaa acctacgaga ttgacattct
     3541 cgactgatct tgattgatcg gtaagtcttt tgtaatttaa ttttcttttt gattttattt
     3601 taaattgtta tctgtttctg tgtatagact gtttgagatc ggcgtttggc cgactcattg
     3661 tcttaccata ggggaacgga ctttgtttgt gttgttattt tatttgtatt ttattaaaat
     3721 tctcaacgat ctgaaaaagc ctcgcggcta agagattgtt ggggggtgag taagtacttt
     3781 taaagtgatg atggttacaa aggcaaaagg ggtaaaaccc ctcgcctacg taagcgttat
     3841 tacgcccgtc tgtacttata tcagtacact gacgagtccc taaaggacga aacgggagaa
     3901 cgctagccac caccaccacc accacgtgtg aattacaggt gaccagctcg aatttccccg
     3961 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     4021 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     4081 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     4141 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     4201 tgttactaga tcgggaatta aactatcagt gtttgacagg atatattggc gggtaaacct
     4261 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     4321 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     4381 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     4441 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     4501 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     4561 gagacattac gccatgaacg agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     4621 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     4681 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     4741 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     4801 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     4861 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     4921 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     4981 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     5041 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     5101 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     5161 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     5221 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     5281 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     5341 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     5401 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     5461 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     5521 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     5581 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     5641 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     5701 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     5761 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     5821 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     5881 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     5941 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     6001 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     6061 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     6121 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     6181 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     6241 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     6301 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     6361 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     6421 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     6481 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     6541 gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca
     6601 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     6661 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     6721 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     6781 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     6841 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     6901 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     6961 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     7021 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     7081 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     7141 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     7201 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     7261 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     7321 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     7381 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     7441 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     7501 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     7561 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     7621 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     7681 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     7741 aaaaggtgtc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     7801 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     7861 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     7921 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     7981 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     8041 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     8101 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     8161 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8221 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8281 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     8341 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     8401 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     8461 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9001 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9061 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9241 cgctcagtgg aacgaaaact cacgttaagg gctgatgaat cccctaatga tttttatcaa
     9301 aatcattaag ttaaggtaga tacacatctt gtcatatgat caaatggttt cgccaaaaat
     9361 caataatcag acaacaaaat gtgcgaactc gatattttac acgactctct ttaccaattc
     9421 tgccccgaat tacacttaaa acgactcaac agcttaacgt tggcttgcca cgccttactt
     9481 gactgtaaaa ctctcactct taccgaactt ggccgtaacc tgccaaccaa agcgagaaca
     9541 aaacataaca tcaaacgaat cgaccgattg ttaggtaatc gtcacctcca caaagagcga
     9601 ctcgctgtat accgttggca tgctagcttt atctgttcgg gcaatacgat gcccattgta
     9661 cttgttgact ggtctgatat ccgtgagcaa aaacggctta tggtattgcg agcttcagtc
     9721 gcactacacg gtcgttctgt tactctttat gagaaagcgt tcccgctttc agagcaatgt
     9781 tcaaagaaag ctcatgacca atttctagcc gaccttgcga gcattctacc gagtaacacc
     9841 acaccgctca ttgtcagtga tgctggcttt aaagtgccat ggtataaatc cgttgagaag
     9901 ctgggttggt actggttaag tcgagtaaga ggaaaagtac aatatgcaga cctaggagcg
     9961 gaaaactgga aacctatcag caacttacat gatatgtcat ctagtcactc aaagacttta
    10021 ggctataaga ggctgactaa aagcaatcca atctcatgcc aaattctatt gtataaatct
    10081 cgctctaaag gccgaaaaaa tcagcgctcg acacggactc attgtcacca cccgtcacct
    10141 aaaatctact cagcgtcggc aaaggagcca tggattctag caactaactt acctgttgaa
    10201 attcgaacac ccaaacaact tgttaatatc tattcgaagc gaatgcagat tgaagaaacc
    10261 ttccgagact tgaaaagtcc tgcctacgga ctaggcctac gccatagccg aacgagcagc
    10321 tcagagcgtt ttgatatcat gctgctaatc gccctgatgc ttcaactaac atgttggctt
    10381 gcgggcgttc atgctcagaa acaaggttgg gacaagcact tccaggctaa cacagtcaga
    10441 aatcgaaacg tactctcaac agttcgctta ggcatggaag ttttgcggca ttctggctac
    10501 acaataacaa gggaagactc actcgtggct gcaaccctgc ttactcaaaa tctattcaca
    10561 catggttacg ttttggggaa attatgaggg gatctctcag cgttaaggga ttttggtcat
    10621 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
    10681 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
    10741 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
    10801 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
    10861 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
    10921 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
    10981 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
    11041 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
    11101 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
    11161 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
    11221 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
    11281 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
    11341 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
    11401 ttccggtgat attctcattt tagccat
//
