LOCUS       sequence-368559-       11092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused 
            to trucated flowering locus T (BsaI-domesticated) which targets 
            phytoene desaturase (PDS) of Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQDKN0760
FEATURES             Location/Qualifiers
     source          1..11092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    425..449
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        978..1323
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1266..1286
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2926..3001
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3573..3590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3625..3877
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3899..3923
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5224..5853
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6282..7355
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7421..7615
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7851..7873)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7959..8099
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8014..8033
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8114..8131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8285..8873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8365..8384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10298..11092)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
       61 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
      121 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
      181 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
      241 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      301 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      361 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      421 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      481 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcctaggcca ccatgttggg
      541 cccggcgcgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac actctcgtct
      601 actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac
      661 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
      721 aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg
      781 ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga
      841 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgatg
      901 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
      961 gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     1021 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc
     1081 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt
     1141 ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc
     1201 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     1261 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     1321 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat
     1381 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc
     1441 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac
     1501 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt
     1561 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac
     1621 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg
     1681 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt
     1741 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg
     1801 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg
     1861 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg
     1921 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga
     1981 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac
     2041 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg
     2101 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag
     2161 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga
     2221 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg
     2281 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt
     2341 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact
     2401 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc
     2461 cagcgagtgg aggtccgata cgtcctaatc cctagggatt taaggacgtg aactctgttg
     2521 agatctctgt gaaattcaga gggtgggtga taccatattc actgatgcca ttagcgacat
     2581 ctaaataggg ctaattgtga ctaatttgag ggaatttcct ttaccattga cgtcagtgtc
     2641 gttggtagca tttgagtttc ggcacacaag gttaaaaacg ctgtagtaat acatgcgcaa
     2701 gaacaggctg agcatcttgt tctggggttt cacactatct ttagagaaag tgttaagtta
     2761 attaagttat cttaattaag agcataatta tactgatttg tctctcgttg atagagtcta
     2821 tcattctgtt attaaaaatt tgacaactcg gtttgctgac ctactggtca ctgtatcact
     2881 tacccgagtt aacgagtcta gaaacttggt agtagcgact ccatggtttt agagctagaa
     2941 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     3001 ctccctagtc tataaatata agagatcctc ttatagtaag cagagttgtt ggagacgttc
     3061 ttgatccgtt taatagatca atcactctaa aggttactta tggccaaagt gccgggcatg
     3121 tcccgaagac attaaactac ggttctttaa gtagatccgt gtctgaagtt ttaggttcaa
     3181 tttaaaccta cgagattgac attctcgact gatcttgatt gatcggtaag tcttttgtaa
     3241 tttaattttc tttttgattt tattttaaat tgttatctgt ttctgtgtat agactgtttg
     3301 agatcggcgt ttggccgact cattgtctta ccatagggga acggactttg tttgtgttgt
     3361 tattttattt gtattttatt aaaattctca acgatctgaa aaagcctcgc ggctaagaga
     3421 ttgttggggg gtgagtaagt acttttaaag tgatgatggt tacaaaggca aaaggggtaa
     3481 aacccctcgc ctacgtaagc gttattacgc ccgtctgtac ttatatcagt acactgacga
     3541 gtccctaaag gacgaaacgg gagaacgcta gccaccacca ccaccaccac gtgtgaatta
     3601 caggtgacca gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
     3661 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     3721 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     3781 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     3841 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg
     3901 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     3961 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
     4021 gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
     4081 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
     4141 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
     4201 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
     4261 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
     4321 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
     4381 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
     4441 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
     4501 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
     4561 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
     4621 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc
     4681 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
     4741 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
     4801 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
     4861 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
     4921 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
     4981 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct
     5041 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
     5101 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     5161 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     5221 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     5281 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     5341 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     5401 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     5461 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     5521 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     5581 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     5641 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     5701 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     5761 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     5821 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     5881 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     5941 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     6001 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     6061 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     6121 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     6181 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg
     6241 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
     6301 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     6361 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     6421 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     6481 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     6541 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     6601 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     6661 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     6721 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     6781 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     6841 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     6901 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     6961 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     7021 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
     7081 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     7141 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     7201 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     7261 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     7321 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     7381 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg
     7441 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     7501 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     7561 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     7621 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
     7681 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
     7741 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
     7801 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7861 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7921 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     7981 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     8041 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8101 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8161 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8221 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8701 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8761 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8821 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8881 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggctga
     8941 tgaatcccct aatgattttt atcaaaatca ttaagttaag gtagatacac atcttgtcat
     9001 atgatcaaat ggtttcgcca aaaatcaata atcagacaac aaaatgtgcg aactcgatat
     9061 tttacacgac tctctttacc aattctgccc cgaattacac ttaaaacgac tcaacagctt
     9121 aacgttggct tgccacgcct tacttgactg taaaactctc actcttaccg aacttggccg
     9181 taacctgcca accaaagcga gaacaaaaca taacatcaaa cgaatcgacc gattgttagg
     9241 taatcgtcac ctccacaaag agcgactcgc tgtataccgt tggcatgcta gctttatctg
     9301 ttcgggcaat acgatgccca ttgtacttgt tgactggtct gatatccgtg agcaaaaacg
     9361 gcttatggta ttgcgagctt cagtcgcact acacggtcgt tctgttactc tttatgagaa
     9421 agcgttcccg ctttcagagc aatgttcaaa gaaagctcat gaccaatttc tagccgacct
     9481 tgcgagcatt ctaccgagta acaccacacc gctcattgtc agtgatgctg gctttaaagt
     9541 gccatggtat aaatccgttg agaagctggg ttggtactgg ttaagtcgag taagaggaaa
     9601 agtacaatat gcagacctag gagcggaaaa ctggaaacct atcagcaact tacatgatat
     9661 gtcatctagt cactcaaaga ctttaggcta taagaggctg actaaaagca atccaatctc
     9721 atgccaaatt ctattgtata aatctcgctc taaaggccga aaaaatcagc gctcgacacg
     9781 gactcattgt caccacccgt cacctaaaat ctactcagcg tcggcaaagg agccatggat
     9841 tctagcaact aacttacctg ttgaaattcg aacacccaaa caacttgtta atatctattc
     9901 gaagcgaatg cagattgaag aaaccttccg agacttgaaa agtcctgcct acggactagg
     9961 cctacgccat agccgaacga gcagctcaga gcgttttgat atcatgctgc taatcgccct
    10021 gatgcttcaa ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag gttgggacaa
    10081 gcacttccag gctaacacag tcagaaatcg aaacgtactc tcaacagttc gcttaggcat
    10141 ggaagttttg cggcattctg gctacacaat aacaagggaa gactcactcg tggctgcaac
    10201 cctgcttact caaaatctat tcacacatgg ttacgttttg gggaaattat gaggggatct
    10261 ctcagcgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
    10321 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
    10381 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
    10441 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
    10501 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
    10561 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
    10621 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
    10681 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
    10741 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
    10801 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
    10861 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
    10921 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
    10981 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
    11041 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc at
//
