LOCUS       sequence-368583-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABEmax-SpCas9-NGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han
            R
  TITLE     Efficient precise in vivo base editing in adult dystrophic mice.
  JOURNAL   Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y.
  PUBMED    34140489
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-17"; volume: "12(1):3719. doi"; pages: " 
            10.1038/s41467-021-23996-y"
COMMENT     Sequence Label: pCMV_ABEmaxNG‐NGA
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(549..1340,1341..1409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1172..1191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1410..1514)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1593..1612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1784..2163
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2164..2367
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2318..2338
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2409..2428
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2409..2427
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2477..2497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3686..7786
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7829..7849
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7858..7875
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7898..7915)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7904..8128
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8199..8215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8199..8215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8212..8234)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8223..8239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8247..8253,8254..8271,8272..8277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8292..8313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8430..8447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8601..8811,1..378))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8681..8700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
       61 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
      181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
      241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
      301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
      361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cactcagtgg
      421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
      481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
      541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
      601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
      661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
      721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     1021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     1081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     1141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     1201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     1261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     1321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     1381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     1441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     1561 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     1621 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     1681 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     1741 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     1801 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1861 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1921 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1981 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2041 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2101 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2161 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2221 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2281 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2341 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc
     2401 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
     2461 aagcgagttc gagtcaccaa agaagaagcg gaaagtctct gaagtcgagt ttagccacga
     2521 gtattggatg aggcacgcac tgaccctggc aaagcgagca tgggatgaaa gagaagtccc
     2581 cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga gagggatgga acaggccaat
     2641 cggccgccac gaccctaccg cacacgcaga gatcatggca ctgaggcagg gaggcctggt
     2701 catgcagaat taccgcctga tcgatgccac cctgtatgtg acactggagc catgcgtgat
     2761 gtgcgcagga gcaatgatcc acagcaggat cggaagagtg gtgttcggag cacgggacgc
     2821 caagaccggc gcagcaggct ccctgatgga tgtgctgcac caccccggca tgaaccaccg
     2881 ggtggagatc acagagggaa tcctggcaga cgagtgcgcc gccctgctga gcgatttctt
     2941 tagaatgcgg agacaggaga tcaaggccca gaagaaggca cagagctcca ccgactctgg
     3001 aggatctagc ggaggatcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc
     3061 agagagctcc ggcggctcct ccggaggatc ctctgaggtg gagttttccc acgagtactg
     3121 gatgagacat gccctgaccc tggccaagag ggcacgcgat gagagggagg tgcctgtggg
     3181 agccgtgctg gtgctgaaca atagagtgat cggcgagggc tggaacagag ccatcggcct
     3241 gcacgaccca acagcccatg ccgaaattat ggccctgaga cagggcggcc tggtcatgca
     3301 gaactacaga ctgattgacg ccaccctgta cgtgacattc gagccttgcg tgatgtgcgc
     3361 cggcgccatg atccactcta ggatcggccg cgtggtgttt ggcgtgagga acgcaaaaac
     3421 cggcgccgca ggctccctga tggacgtgct gcactacccc ggcatgaatc accgcgtcga
     3481 aattaccgag ggaatcctgg cagatgaatg tgccgccctg ctgtgctatt tctttcggat
     3541 gcctagacag gtgttcaatg ctcagaagaa ggcccagagc tccaccgact ccggaggatc
     3601 tagcggaggc tcctctggct ctgagacacc tggcacaagc gagagcgcaa cacctgaaag
     3661 cagcgggggc agcagcgggg ggtcagacaa gaagtacagc atcggcctgg ccatcggcac
     3721 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     3781 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     3841 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     3901 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     3961 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     4021 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     4081 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     4141 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     4201 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     4261 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     4321 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     4381 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     4441 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     4501 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     4561 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     4621 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     4681 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     4741 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     4801 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     4861 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     4921 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     4981 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     5041 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     5101 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     5161 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     5221 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     5281 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     5341 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     5401 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     5461 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     5521 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     5581 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     5641 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     5701 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     5761 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     5821 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     5881 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     5941 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     6001 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     6061 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     6121 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     6181 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     6241 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     6301 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     6361 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     6421 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     6481 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     6541 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     6601 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     6661 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     6721 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     6781 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     6841 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     6901 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     6961 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tccggcccaa
     7021 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     7081 cttcgtcagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     7141 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     7201 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     7261 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     7321 gctggcctct gcccgctttc tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     7381 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     7441 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     7501 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     7561 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     7621 taccctgacc aatctgggag cccctcgggc cttcaagtac tttgacacca ccatcgaccg
     7681 gaagcagtac cggagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     7741 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa
     7801 aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat
     7861 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     7921 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7981 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8041 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8101 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     8161 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8221 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8281 tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8341 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8401 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8461 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8521 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag g
//
