LOCUS       sequence-36859-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Hong JH, Hwang ES, McManus MT, Amsterdam A, Tian Y, Kalmukova R, 
            Mueller E, Benjamin T, Spiegelman BM, Sharp PA, Hopkins N, Yaffe MB
  TITLE     TAZ, a transcriptional modulator of mesenchymal stem cell 
            differentiation.
  JOURNAL   Science. 2005 Aug 12;309(5737):1074-8.
  PUBMED    16099986
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-08-12"; volume: "309(5737)"; pages: "1074-8"
COMMENT     Sequence Label: pcDNA3.1/HisC-mTAZ
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..121
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             125..157
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(161..169,170..184)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttac catggggggt tctcatcatc atcatcatca
      121 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga
      181 taaggtacca ggatccagtg tggtggaatt catgaatccg tcctcggtgc cccatccgct
      241 cccgccgcca gggcagcaag tcatccacgt cacgcaggac ctggacaccg acctcgaagc
      301 cctcttcaac tctgtcatga accccaagcc cagctcatgg cggaaaaaga tcctcccgga
      361 gtccttcttt aaggagcccg attccggctc gcactcgcgc caatccagca cagactcatc
      421 aggcggccac ccggggcctc gactagctgg cggcgcgcag cacgtccgct cgcactcgtc
      481 gcccgcatcc ctgcagctgg gcaccggtgc gggagccgct ggaggccctg cacagcagca
      541 tgcacatctc cgccagcagt cctatgacgt gaccgacgag ctgccgttgc cccccgggtg
      601 ggagatgacc ttcacggcca ctggccagag atacttcctt aatcacatag agaaaatcac
      661 cacatggcaa gaccccagga aggtgatgaa tcagcctctg aatcatgtga acctccaccc
      721 gtccatcact tccacctcgg tgccacagag gtccatggca gtgtcccagc cgaatctcgc
      781 aatgaatcac caacaccagc aagtcgtggc cactagcctg agtccacaga accacccgac
      841 tcagaaccaa cccacagggc tcatgagtgt gcccaatgc
//
