LOCUS       sequence-368595-        6940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human mitochondrial DNA ND4 target TALE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Mok YG, Lee JM, Chung E, Lee J, Lim K, Cho SI, Kim JS
  TITLE     Base editing in human cells with monomeric DddA-TALE fusion 
            deaminases.
  JOURNAL   Nat Commun. 2022 Jul 12;13(1):4038. doi: 10.1038/s41467-022-31745-y.
  PUBMED    35821233
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-12"; volume: "13(1):4038. doi"; pages: " 
            10.1038/s41467-022-31745-y"
COMMENT     Sequence Label: pAAV-ND4 Right-GSVG-UGI
FEATURES             Location/Qualifiers
     source          1..6940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     enhancer        171..550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        551..754
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     705..725
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    799..823
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      824..833
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             923..949
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             953..979
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             983..1009
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3677..3928
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     promoter        complement(3938..3956)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3939..3956)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3976..3993)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3982..4189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4197..4337
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4197..4326
                     /label=AAV2 ITR
     rep_origin      4412..4867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4499..4518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4709..4730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4884..4903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5003..5025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5063..5081)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5149..5253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5254..5322,5323..6114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5472..5491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6285..6873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6774..6793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgatgt acgggccaga tatacgcgtt gacattgatt
      181 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      241 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      301 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      361 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      421 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      481 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      541 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      601 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      661 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      721 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      781 ttactggctt atcgaaattc aagtttgtac aaaaaagcag gctgccacca tggctcttag
      841 tcgagccgtc tgtggtactt cccgacagct tgctccagtc ttggggtacc ttggctcccg
      901 acaaaagcac tctctccctg attaccctta cgatgtacct gactacgccg gctaccctta
      961 cgacgtacca gattacgctg gatatccata cgatgtccct gattatgctg ggatccgaat
     1021 tcaagatctg cgtaccctgg gttacagcca gcagcagcag gagaagatca agccaaaggt
     1081 gcgcagcacc gtggcccagc accatgaagc tctggtgggt cacggcttca cccacgcgca
     1141 catcgtggct ctgagccagc acccagccgc gctgggtacc gtggccgtga aatatcagga
     1201 catgattgct gccctgccag aggctaccca tgaagcgatt gtgggtgtgg gcaagcagtg
     1261 gagcggtgct cgtgcgctgg aggccctgct gaccgtggcc ggtgaactgc gtggcccgcc
     1321 gctgcagctg gataccggcc agctgctgaa aatcgcgaaa cgtggcggtg tgaccgctgt
     1381 ggaagctgtg catgcctggc gtaatgctct gaccggtgcc ccgctgaacc tgaccccgga
     1441 acaagtggtt gcgattgcgt cccatgatgg tggtaaacaa gccctggaga ccgttcaagc
     1501 tctgctgcca gtgctgtgcc aggctcatgg tctgaccccg cagcaggtgg tggctattgc
     1561 cagccatgat ggcggcaagc aggctctgga gaccgtgcag cgtctgctgc cggtgctgtg
     1621 ccaagcccat ggcctgaccc cgcagcaagt tgtggctatt gccagcaacg gcggtggcaa
     1681 acaggcgctg gaaaccgtgc agcgcctgtt accggtgctg tgccaagcgc atggtctgac
     1741 cccggagcag gtggtggcca tcgctagcaa caacggtggc aagcaggccc tggaaaccgt
     1801 gcaggcgctg ttgccggtgc tgtgccaggc ccatggctta accccggaac aagtggtggc
     1861 cattgcgagc aatggtggcg gtaaacaggc tctggaaacc gtgcaggccc tgcttccggt
     1921 gctgtgccag gcccatgggc tgaccccgga acaagttgtg gctatcgcca gcaacatcgg
     1981 cggcaagcag gcgctggaga ccgttcaggc tctgttaccg gtgctgtgcc aggcgcatgg
     2041 cctgaccccg gaacaggtgg tggcgatcgc tagcaacatt ggcggtaaac aggccctgga
     2101 gaccgttcag cgcctgctcc cggtgctgtg ccaggcccat ggtctgaccc cggaacaggt
     2161 tgttgctatt gccagcaaca acggcggcaa gcaggccctg gagaccgtgc aggcgctgct
     2221 accggtgctg tgccaggccc atggactgac cccggagcag gttgtggcca tcgcgtccaa
     2281 tggcggtggc aaacaggctc tggagaccgt tcagcgtctg ttaccggtgc tgtgccaggc
     2341 acatggcctg accccggagc aagttgttgc catcgctagc aacattggtg gcaagcaggc
     2401 gctggaaacc gttcagcgcc tgctaccggt gctgtgccag gctcatggct taaccccgga
     2461 gcaggttgtc gccattgcca gcaacaatgg tggcaaacag gctctggaaa ctgtgcaggc
     2521 cctgctaccg gtgctgtgcc aggcccatgg gttaaccccg gaacaggttg tggccattgc
     2581 ctccaataac ggtggcaagc aggcgctgga aacggtgcag gctctgcttc cggtgctgtg
     2641 ccaggctcat gggctgaccc cggagcaagt ggttgctatt gcgtccaaca ttggtggcaa
     2701 acaggccctg gaaaccgttc aggcgctgct cccggtgctg tgccaggccc atgggctaac
     2761 cccggaacag gtggttgcca ttgcctccaa caatggtggc aagcaggccc tggaaacggt
     2821 tcagcgtctg cttccggtgc tgtgccaggc ccatgggctt accccgcagc aagttgttgc
     2881 tatcgccagc aatattggtg gcaaacaggc tctggaaacg gtgcagcgcc tgctaccggt
     2941 gctgtgccag gctcatggtt taaccccgca gcaggtggtt gcgattgcct ccaacaacgg
     3001 tggcaagcag gcgctggaaa ctgttcagcg tctgctcccg gtgctgtgcc aggctcacgg
     3061 cctgaccccg cagcaagtgg tggctatcgc ctccaacggt ggtggtcgcc cggctctgga
     3121 aagcattgtg gcgcagctga gccgtccaga cccggccctg gcggctctga ccaatgatca
     3181 cctggtggcc ctggcctgcc tgggtggccg tccggctctg gatgccgtga agaaaggtct
     3241 gggcggatcc ggcagctacg ccctgggtcc gtatcagatt agcgccccgc agctgccagc
     3301 atacaatggt cagaccgtgg gtaccttcta ctatgtgaac gacgctggcg gtctggaggg
     3361 caaggtgttt agcagcggcg gtccaacccc gtacccaaac tatgccaatg ccggtcatgt
     3421 ggagagtcag agcgccctgt tcatgcgtga taacggcatc agcgagggtc tggtgttcca
     3481 caacaacccg gaaggcacct gcggtttttg cgtgaacatg accgagaccc tgctgccgga
     3541 aaacgcgaaa atgaccgtgg tgccgccgga aggtgtcatt ccagtgaagc gcggcgctac
     3601 cggtgaaacc aaagtgttta ccggtaacag caacggcccg aagagcccga ccaaaggcgg
     3661 ttgctctggc ggctcaacta atctgagcga catcattgag aaggagactg ggaaacagct
     3721 ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa
     3781 gccagagtct gacatcctgg tgcacaccgc ctacgacgag tccacagatg agaatgtgat
     3841 gctgctgacc tctgacgccc ccgagtataa gccttgggcc ctggtcatcc aggattctaa
     3901 cggcgagaat aagatcaaga tgctgtgagg gccctattct atagtgtcac ctaaatgcta
     3961 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4021 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4081 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4141 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggag ggccgcagga
     4201 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4261 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4321 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4381 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4441 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     4501 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4561 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4621 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4681 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4741 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     4801 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4861 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4921 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4981 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5041 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5101 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6901 ttcctggcct tttgctggcc ttttgctcac atgtgtcgac
//
