LOCUS       sequence-36863-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA, 
            Sancak Y, Bao XR, Strittmatter L, Goldberger O, Bogorad RL, 
            Koteliansky V, Mootha VK
  TITLE     Integrative genomics identifies MCU as an essential component of the
            mitochondrial calcium uniporter.
  JOURNAL   Nature. 2011 Jun 19;476(7360):341-5. doi: 10.1038/nature10234.
  PUBMED    21685886
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-19- 19"; volume: "476"; issue: "7360"; 
            pages: "341-5"
COMMENT     Sequence Label: pDEST40-MCU-V5-HIS
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(33..74)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tactcatggt gatggtgatg atgaccggta cgcgtagaat cgagaccgag gagagggtta
       61 gggataggct taccttcgaa ccgcgggccc tctagatcaa ccactttgta caagaaagtt
      121 gggcaatctt tttcaccaat ttgtcggaga ggcagatgta cttgtaatgg gtctctcagt
      181 ctcttaaggt ccatttctgc ctgagcaatt gcatccttga gttgattgta tttctctagg
      241 tcaaaacgtg actttttggc tcctttatgg aaaaatagta agtattgtct gtctctggct
      301 tctggataaa catattcctg gcgtgtcatt acaaaatatg catacattgc catggcactt
      361 ccataagtga tgaagtatgt tactggctcc atgatgtccc aggaatattc ccaccaggta
      421 agccgggcca aaatgccaaa ctgtgtggcc atgtaggcaa ggccacccca tagcaccaaa
      481 gtggtcctct tctcagcttt tctgctaatc tcaattcgta ccttttccag gggagccagc
      541 tgctctttga gatcctctag tctttcaata agctcccttt ccttgtttaa ctggtgctgc
      601 tcaatgcaca gtgtggtgta tagttgctgg accaatgtct ttacatcatt cagcgttgct
      661 gcattttcat gacttaagag gtctcttttt ggtggtcgta cgtggtatgt taagtcatta
      721 atgaccagct taaagtcatc aaggaggagg aggtctattc ctgttgaagc agcaacgcga
      781 acaccatctg gtgaatagat agcaactctg tcaattcccc gatcctcttc ttgcagttgt
      841 cgtaaaaata caccaacaga gtcagagata ggcttgagtg tgaactgaca gcgttcacgc
      901 cggatgtagc ctccagantc cagacntgac
//
