LOCUS       sequence-36864-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 1329 (=HL 865 + pHL 344)
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        293..397
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(616..635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgggaagtc ttctccttnc gcatgctatc atgaactttc agcgtattct gactcataag
       61 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt
      121 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
      181 tgcaagcgtg ttgaactggt tccggtgtcg acagtatctt gttatccgct cacaatgtca
      241 atgttatccg ctcacattta tgacgtcagg tggcactttt cggggaaatg tgcgcggaac
      301 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      361 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      421 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      481 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      541 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      601 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      661 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      721 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      781 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      841 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      901 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg cc
//
