LOCUS       sequence-36865-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 839 (=HL 716 + pHL 108)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(199..217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        285..389
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(608..627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcatgctagc tgggaccagg agggacagta ctttaacttt catgttatta accctctgtt
       61 atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat taatcagtcg
      121 gcaagtcgac agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact
      181 cgaggtgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      241 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      361 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      421 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      481 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      541 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      601 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      661 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      721 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      781 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggactacgct ttgcca
//
