LOCUS       sequence-368869-        7206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human Plekhh1-CTer labelled with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Prosperi MT, Pernier J, Lachuer H, Coudrier E
  TITLE     Plekhh1, a partner of myosin 1 and an effector of EphB2, controls 
            the cortical actin network during cell repulsion.
  JOURNAL   J Cell Sci. 2021 Dec 1;134(23). pii: 272686. doi: 
            10.1242/jcs.258802. Epub 2021 Dec 6.
  PUBMED    34723325
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-12-1"; volume: "134(23). pii: 272686. doi"; 
            pages: " 10.1242/jcs.258802. Epub 2021 Dec 6"
COMMENT     Sequence Label: EGFP-Plekhh1-CTer
FEATURES             Location/Qualifiers
     source          1..7206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3994..4115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4031..4050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4085..4104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4122..4577)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4259..4280)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4471..4490
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4604..4708
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4710..5067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4710..4901
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4733..4753)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4918..5053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5102..5896
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5102..5896
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5156..5175)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5766..5785
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6084..6103)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6128..6175
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6504..7092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6993..7012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgacta cgccatcccc
     1381 ccggacgcct gctcactgga cagtgactac tcagagcctg agcacaaact gcagcgcacc
     1441 tcatcctact ccaccgacgg gctgggcctg ggcggggagt cactggagaa gtcgggctac
     1501 ctgctgaaaa tggggagcca ggtgaagacg tggaagaggc gctggtttgt cctgagacag
     1561 ggacagatta tgtactacaa gtccccgagt gatgtcatcc ggaaacctca aggccaagtg
     1621 gatctgaact cccgctgcca aattgttcga ggggagggtt cacagacgtt tcagctcatc
     1681 tctgagaaga aaacctacta cctgacggcc gattcaccca gcctgctgga ggagtggatc
     1741 cgagtactcc agagcctgct gaaggtgcag gccaccgggc ctccagctct gcttcggggt
     1801 ggcaccaagc ccaccgtgaa gggctggctg accaaggtaa agcatggcca ctccaaggtg
     1861 gtctggtgcg ctcttgttgg gaaaatcttc tactactatc ggagccatga ggacaagcga
     1921 cccctgggct gcctgcctgt gcgggatgcg cacatagagg aagtagatcg atcctgtgac
     1981 tcagacgagg actatgaggc tggaggaacc agacggttgc tttcctccca ctgcaccctg
     2041 gtgatccacc ccacagagca cagccccacc tacctcctca ttggcaccaa gcatgaaaag
     2101 gatacgtggc tctaccacct cacagtggct gcaggtggca gcagtgccaa ggtgggcact
     2161 gcctatgagc agctcattgg aaaactgatg gatggtgaag gagatccaga ttcgccgctc
     2221 tggaggcacc ccatgctgtg ctacagcaaa gacggcctat acgcctccct caccaccctg
     2281 ccctctgagg ccttgcagac ggaggccctc aagctcttca agtcctgcca gctcttcatc
     2341 aacgtgccgg tggaagctgc ctcggtggac taccatgtgt ccctggccca gaccgcactg
     2401 caggtctgcc tggttcaccc cgagctgcag agtgagatct actgccaact catgaagcag
     2461 accagctgcc gcccacctca gaagtactcc ctcatgcagt gctggcagct cctcgctctg
     2521 tgtgctccac ttttcctgcc tcagcatcac ttcctctggt atgtcaagca gcagctccaa
     2581 cgccatgcag atcccagaag tgaaactggc cagtatgcca cctactgcca gcgggcagtg
     2641 gagcggaccc tgcggaccgg ggagcgggaa gccaggccat cgcgcatgga agtggtgtcc
     2701 atcctgctgc gtaacccctt ccaccactcc ttgcccttca gcatccccgt gcactttacc
     2761 aacgggactt accatgtggt tggttttgac ggctcttcca cggttgatga gttcctccag
     2821 cggctgaacc aggagatagg catgagaaag ccatcccact ctggctttgc cctcttcacg
     2881 gacgatccct cgggcaggga cctggagcac tgcctgcagg gaagtgtcaa gatctgtgat
     2941 gccatctcca agtgggaaca agccatgaag gagctgcacc ccggaaagtc tgagggtggg
     3001 acacgcgtcg tgaagctgat gtacaagaac aggctgtact ttcgcagtca agtcaaaggg
     3061 gagacggacc gagaacggct gctccttgcc tctcaaacca gtagagagat agtggcaggg
     3121 aggtttccta tcaacaagga attggctctt gagatggctg ccctgatggc ccaggtagaa
     3181 tatggggact tggagaagcc tgccctgcca ggccctggag gcacatcccc tgccaaggct
     3241 cagcatcttc tccagcaggt cctagacagg ttccacccca ggcgctatag acatggggcc
     3301 cccgctgaac agctgaggca cctggcagat atgttgacca caaaatgggc aacattgcaa
     3361 ggatgctccc ctcctgagtg catccgcatc tacctgaccg tggccaggaa atggcctttc
     3421 tttggtgcta aactttttgc tgctcagcct gcccagctgt cttccaagga gaacgctctg
     3481 gtgtggattg ctgtgaatga ggatggcgtc agcatcctgg accacaacac tatgcaagtg
     3541 cacatcactt acccctactc ttcagtgaca acgtttggtg gctgcaggga tgacttcatg
     3601 cttgtgatta gatctatccc agacaagagc tctggaaaaa gccacattga gaagttgatc
     3661 ttccggatgg ctgctcccaa gattgcagaa gccaccttca tcatggccag ctatatgaac
     3721 cattgcacta caactgtgaa cccccccacc aacccacccg gagcctgcca gctgtgggaa
     3781 ctggatggac gacagttctt ttcttctgtt tcctgtgcta ccaaggggcc aacgttgctg
     3841 tgagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     3901 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3961 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4021 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4081 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     4141 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4201 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4261 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4321 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4381 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4441 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4501 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4561 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4621 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4681 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4741 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4861 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4921 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4981 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5041 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     5101 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5161 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5221 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5281 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5341 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5401 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5461 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5521 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5581 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5641 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5701 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5761 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5821 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5881 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5941 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6001 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6061 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     6121 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     6181 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     6241 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6301 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     6361 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6421 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6901 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7141 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7201 atgcat
//
