LOCUS       sequence-368870-        7482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmFMR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7482)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmFMR1_B
FEATURES             Location/Qualifiers
     source          1..7482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4788..4829
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4839..4856
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4879..4896)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4929..5063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4953..4972)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5007..5026
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5664..6252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5744..5763)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6423..7214,7215..7283))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7046..7065
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7284..7388)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7456..7474
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgatggaag atctcctcgt ggaagttcgg ctcgacaatg gcgcctacta
     2761 caagggccag gtgacagctg tcgccgatga cggcatcttt gtggatgtgg acggcgtgcc
     2821 cgagagtatg aaatatccat ttgtggacgt tcgcctgccg cccgaggaaa ccgttgaggt
     2881 ggccgcaccg atcttcgagg agggcatgga agtggaggtg ttcacgcgca cgaacgatcg
     2941 ggaaacctgc ggctggtggg tgggcattat taaaatgcgc aaagcggaaa tctatgcggt
     3001 cgcgtacatc ggttttgaga cgtcctacac cgagatctgt gaattgggtc gtctgcgcgc
     3061 caaaaactcg aatccgccaa ttacagccaa gaccttctac cagttcacgc tgcccgtgcc
     3121 cgaggagttg cgcgaagaag cccagaagga tggtatacac aaggagtttc agcgcacaat
     3181 cgatgccggc gtgtgcaact acagtcgcga tctggacgcc ctgattgtca tctccaagtt
     3241 tgagcacact cagaagcgag ccagtatgct caaggatatg cattttcgta atctctcaca
     3301 aaaggttatg ctgttgaaaa ggactgaaga agctgcccgt cagctcgaaa caactaaact
     3361 gatgagccgt ggaaactacg ttgaggagtt ccgtgtgcgt gacgatctca tgggtttggc
     3421 gattggctcg catggctcga atattcaagc ggcacgcact gtggacggcg tcaccaacat
     3481 cgagctggag gagaagtcct gcacattcaa aatcagcgga gagaccgagg agtctgtgca
     3541 gcgtgcccgc gcgatgctcg aatacgccga ggagttcttc caggtgccca gggagttggt
     3601 gggcaaggtg attggcaaga atgggcgcat tatccaggag attgtggaca agagtggcgt
     3661 gtttcgaatc aagatcgctg gcgacgatga acaggatcag aacataccac gtgagctggc
     3721 gcatgtaccc tttgtgttca ttggcaccgt ggagagcatt gcaaatgcca aagtgctgtt
     3781 ggagtatcat ctgtcgcacc tgaaggaagt agaacagttg cgtcaggaga agatggagat
     3841 tgatcagcag cttcgcgcca tccaggaatc ctccatgggc tccacacaga gcttcccagt
     3901 gacgcggcgc tctgagcgcg gctacagcag tgacattgag tcggtgcgct ctatgcgcgg
     3961 cggtggtggc ggccagcgtg gtcgtgtacg cggacgtggt ggtggcggtc ctggaggtgg
     4021 caatggcctg aaccagcgct accacaacaa tcgccgcgac gaggatgact acaactcgcg
     4081 gggcgatcat cagcgtgacc agcagcgtgg ctacaacgat cgcggcggtg gtgacaacac
     4141 tggcagctat cgtggaggcg gtggtggcgc cggtggtcct ggcaacaaca ggcgcggcgg
     4201 aatcaaccgc cggccgccac gcaacgatca gcagaatggc agggactatc agcaccacaa
     4261 ccacaccacc gaagaggtgc gggagacccg tgaaatgagc agcgtggaga gagcggacag
     4321 taactcatcg tacgaaggta gctcgcggag gcgcagaagg cagaagaaca acaatagacc
     4381 cagtaacaca aacggagctg ttgccaataa caacaacaaa cctcagtcgg cgcagcagcc
     4441 acaacagcag cagccgccgg cgcctgggaa taaggcggca ctaaatgccg gagatgccag
     4501 caaacagaac agcggcaatg cgaacgccgc tggcggtgcg agcaagccga aggatgcctc
     4561 acgcaacggc gacaagcagc aggcgggcac acagcagcaa cagccatcgc aggtacaaca
     4621 gcagcaggcg gcacagcagc agcagccgaa accgcgacgc aacaagaatc gcagcaacaa
     4681 ccacacggac cagccatccg gacagcagca gctggcggag aatgtcaaaa aggagggcct
     4741 ggtcaatggc acgtcctaac tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     4801 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     4861 tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct
     4921 cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4981 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     5041 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     5101 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     5161 attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     5221 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     5281 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     5341 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     5401 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     5461 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     5521 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     5581 aggcaggtag atgacgacca tcagggacag cttcaaggcc agcaaaaggc caggaaccgt
     5641 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5761 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5821 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5881 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5941 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6061 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6121 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6181 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6241 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6301 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6361 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6421 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6481 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6541 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6601 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6661 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6721 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6781 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6841 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6901 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6961 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7021 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     7081 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7141 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7201 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7261 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7321 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7381 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7441 acattaacct ataaaaatag gcgtatcacg aggccctttc gt
//
