LOCUS       sequence-368888-        8091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rat myosin 1b isoform b  domain labelled with Cherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Iuliano O, Yoshimura A, Prosperi MT, Martin R, Knolker HJ, Coudrier 
            E
  TITLE     Myosin 1b promotes axon formation by regulating actin wave 
            propagation and growth cone dynamics.
  JOURNAL   J Cell Biol. 2018 Jun 4;217(6):2033-2046. doi: 
            10.1083/jcb.201703205. Epub 2018 Mar 27.
  PUBMED    29588377
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201703205"; journalName: "J Cell Biol"; date: 
            "2018-06-4- 4"; volume: "217"; issue: "6"; pages: "2033-2046"
COMMENT     Sequence Label: Cherry-Myo1b
FEATURES             Location/Qualifiers
     source          1..8091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      613..622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             619..1326
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(764..782)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1032..1051
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4879..5000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4916..4935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4970..4989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5007..5462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5144..5165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5356..5375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5489..5593
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5595..5952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5595..5786
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5618..5638)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5803..5938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5987..6781
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5987..6781
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6041..6060)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6651..6670
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6969..6988)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7013..7060
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7389..7977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7878..7897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgttt
      601 aaacttaagc ttgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      661 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      721 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      781 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      841 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      901 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      961 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1021 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1081 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1141 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1201 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1261 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1321 tacaagtccg gactcagatc tcgagctcaa gcttcgaatt cggatcctag atatccaccc
     1381 atggccaaga aggaggtaaa atcctcactt ctggacaata tgatcggcgt tggagatacg
     1441 gttctcttgg agcctctcaa cgaggagacg ttcatcgaca acctgaagaa gcgcttcgat
     1501 cacaatgaga tatatacata catcgggagt gtggttatat ctgttaaccc ctaccggtcc
     1561 ttgcccattt attcaccaga gaaagtggaa gattacagaa atagaaactt ctatgaactg
     1621 agccctcaca tctttgcttt gtcggatgaa gcatacagat ccctccgtga tcaagataaa
     1681 gatcagtgca ttctcattac tggggagagt ggagcgggaa aaacagaggc cagcaagctt
     1741 gtgatgtcct atgtcgctgc agtgtgtgga aaaggagcag aagttaatca ggtcaaggaa
     1801 cagcttctcc agtccacccc tgtcctggaa gcttttggaa atgccaagac tgtaaggaat
     1861 gacaattcat ccagattcgg caaatacatg gatattgaat ttgactttaa aggcgatccg
     1921 ctgggaggag tgataagtaa ctatctttta gagaaatctc gtgttgttaa gcagccgaga
     1981 ggtgaaagaa acttccacgt gttctatcag ctgctctcgg gtgcctctga ggaactcctc
     2041 cacaagctta aactggagcg ggatttcagc agatacaact atctgagtct ggactctgcc
     2101 aaagtgaatg gtgtggacga cgctgccaat ttcagaactg tcaggaatgc catgcagatc
     2161 gtgggctttt cggatcctga ggccgagtct gtcctggagg ttgtggcagc tgtcttgaaa
     2221 ctggggaaca tcgagttcaa gcctgaatca cgaatgaacg gtttggatga aagcaaaatc
     2281 aaagacaaaa atgagttaaa agaaatttgt gagttgacca gcatcgatca agtggtttta
     2341 gaaagagcgt tcagtttccg aacggttgag gcgaagcagg agaaagtttc aaccacattg
     2401 aatgtggctc aggcttatta tgcccgtgat gccctggcta aaaacctcta cagccggttg
     2461 ttttcatggt tggtaaatcg aatcaacgaa agcattaagg cacaaaccaa agtgagaaag
     2521 aaggtcatgg gtgttctgga catttatggc tttgagatat ttgaggacaa cagcttcgag
     2581 cagttcatta ttaattattg taatgaaaag cttcaacaaa tcttcattga actgaccctc
     2641 aaagaagagc aagaggaata tattcgtgag gacatagaat ggactcacat tgactacttc
     2701 aacaatgcta tcatttgcga cctaatagaa aacaacacaa acggaatctt ggccatgctg
     2761 gatgaagagt gcctgagacc cggcactgtt acagatgaga ccttcctgga aaagctgaac
     2821 caagtctgtg ccacccacca gcacttcgag agcaggatga gcaaatgttc ccgcttcctc
     2881 aatgacacga ccctgcctca cagctgcttc agaatccagc attatgctgg caaggtgcta
     2941 taccaggtgg aagggtttgt tgacaagaac aatgaccttc tgtaccgaga cctgtctcaa
     3001 gccatgtgga aggctggcca tgccctcatc aagtctctgt tccctgaagg gaatcctgca
     3061 aaggtcaacc tgaaaagacc tcctactgca gggtcccagt tcaaggcgtc ggtggccacc
     3121 ctgatgaaaa acctgcagac caagaacccg aactatatca ggtgcatcaa gccgaacgat
     3181 aaaaaggccg ctcacatttt cagtgagagt ctcgtgtgcc atcagatccg gtacctgggt
     3241 cttctggaga acgttcgagt gagacgggca ggctatgcct tcaggcaggc ctatgaacct
     3301 tgtctggaaa gatacaagat gctttgcaaa caaacatggc ctcactggaa gggaccagca
     3361 aggtctggtg tggaagttct gtttaatgag ctagagattc ccgtggaaga gtactccttc
     3421 ggtagatcca agatattcat ccgaaaccca aggacattat tccaattaga agacctaagg
     3481 aagcagcggc tggaggacct ggccactctc attcagaaga tttatcgagg atggaaatgt
     3541 cgtacacatt ttctgctcat gaaaagaagc caagtcgtga ttgccgcctg gtacaggaga
     3601 tacgcgcaac aaaagcgata tcagcagata aagagttcag ccctggtgat tcagtcttac
     3661 atccggggct ggaaggctag aaaaatcctg cgggagctga agcatcagaa acgctgcaag
     3721 gaggcagcca ccaccatcgc agcatactgg cacggcaccc aggcacgaag ggaattgaaa
     3781 cgcttgaagg aggaggctag gcgtaagcat gcagttgctg tcatttgggc ttactggctt
     3841 ggactgaagg tacgaaggga gtacaggaaa ttctttagag caaatgctgg aaagaaaatc
     3901 tatgagttta cacttcaaag aattgtgcaa aaatacttgt tggaaatgaa aaataaaatg
     3961 ccttccttgt caccaataga taaaaattgg ccatcaagac cttacctatt cttggattcg
     4021 actcacaagg agctaaaaag gatcttccac ttgtggaggt gtaaaaaata cagggatcaa
     4081 ttcacagacc agcagaaact tatttatgaa gagaagctgg aggccagtga actcttcaaa
     4141 gacaagaagg ctttatatcc ttctagtgtt ggtcagccat tccaaggagc ttacctggaa
     4201 atcaacaaga acccaaaata taagaaactc aaagatgcca ttgaagagaa gatcattatt
     4261 gctgaggttg tgaacaaaat caaccgggct aatgggaaga gtacatctcg gattttcctc
     4321 ttaacaaaca ataaccttct ccttgctgac caaaagtcgg ggcagatcaa gtccgaggtc
     4381 cccctggtgg atgtgactaa ggtctccatg agttcacaga atgatggttt ctttgcagta
     4441 cacctcaagg agggttcaga agcagctagt aagggtgact ttctcttcag cagtgaccac
     4501 ctgattgaaa tggcaaccaa gttgtatcgc acgactctta gccaaactaa acagaagctc
     4561 aatatcgaga tttccgatga gttcctggta cagttcagac aggacaaagt atgtgtgaag
     4621 tttattcaag gcaaccagaa aaacgggagc gtgccaacct gcaaacgaaa gaacaacagg
     4681 cttcttgaag tcgctgtccc ttaagtgagg atcaagctta tcgataccgt cgacggtacc
     4741 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     4801 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     4861 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4921 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4981 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     5041 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     5101 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5161 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5221 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5281 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5341 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     5401 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     5461 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5581 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     5641 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5701 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5761 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5881 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5941 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     6001 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6061 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6121 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     6181 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6241 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6301 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6361 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6421 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6481 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     6541 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6601 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6661 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6721 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6781 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     6841 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6901 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     6961 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     7021 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     7081 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     7141 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     7201 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     7261 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7321 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7381 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7441 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7501 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7561 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7621 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7681 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7741 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7801 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7861 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7921 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7981 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8041 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
