LOCUS       sequence-368897-       15124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty pVS1-based INTEGRATE vector with loxP cargo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Aliu E, Lee K, Wang K
  TITLE     CRISPR RNA-guided integrase enables high-efficiency targeted genome 
            engineering in Agrobacterium tumefaciens.
  JOURNAL   Plant Biotechnol J. 2022 Jun 11. doi: 10.1111/pbi.13872.
  PUBMED    35690588
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2022 Jun 11. doi: 10.1111/pbi.13872."
COMMENT     Sequence Label: pKL2310
FEATURES             Location/Qualifiers
     source          1..15124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..39
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             2161..2172
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8474..8492)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8488..8535
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    8681..8714
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             9076..9870
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MAREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPA
                     KRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDL
                     FEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQ
                     PVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10116..10704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10605..10624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10858..10875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10890..11030
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10956..10975)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11116..11138
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11374..11568
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11634..12707)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13136..13765)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     terminator      15015..15042
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(15043..15114)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agctttgaca gctagctcag tcctaggtat aatactagcg tcgacaggat ccgaattcga
       61 gctcggtata aggagatata ccatggcgac aagtttacct acgccctcag caattacgac
      121 ttcggcgtta gagtatgcat tccatactcc cgctcgcaat ctaacgaaat ctcgcggaaa
      181 aaacattcat cgttatgtca gtgtaaagat gagtaagagg attacggtag aatctactct
      241 agagtgtgat gcctgctatc actttgattt tgagccaagt attgttcgct tttgcgctca
      301 accgattcga tttttatatt atctcaatgg tcagtctcac tcctatgttc ctgactttct
      361 agttcaattt gataccaacg agtttgttct atatgaagta aagtcagctt atgctaagaa
      421 caaacctgat tttgatgttg aatgggaggc gaaagtaaaa gcagcaactg aactagggct
      481 agaattggag cttgttgaag agagtgatat tagggatacg gttgtattaa ataatcttaa
      541 gcgcatgcat cgttatgctt cgaaagatga gctgaataac gtacataact ctctcttaaa
      601 aataataaag tacaatggcg cccaatctgc aagatgcttg ggagaacagt tgggtttaaa
      661 aggccgaact gttttaccaa ttttgtgcga tttgctgtca aggtgtttac tcgatacacg
      721 tttggataag cctctatctc ttgaatctcg atttgagttg gccagttatg gctaagaaag
      781 ggttctcaag tttccataga aaagcagtct cttcccaaga tacgcttgaa tcgatagagc
      841 ttgtctctag cgctaattgt ctagaaagtg ttacgtatca agatatatca gcatttcccg
      901 aaacaattgc ggtagagatt aatttccgat taagcattct tcgcttttta gcgcgaaagt
      961 gcgaaaccat tgtggccaaa tcaattgaac cacatcgtgt agagctacag caaaactata
     1021 gtagaaaaat acccagtgca ataacgatat atcgatggtg gcttgctttt cgaaaatcag
     1081 actacaaccc cattagctta gcacctaata tcaaggatag aggtaataga gagacaaaag
     1141 tgtcaacagt tgttgattct attatggaac aggcagttga aagagttata tctggacgaa
     1201 aagtcaatgt tagctctgca tataaacgtg ttcgacgaaa agttcgtcaa tacaatctaa
     1261 ctcatggaac gaaatacacg tatcctaagt acgaatctgt aagaaagcga gtaaaaaaga
     1321 aaaccccatt tgagttatta gccgcaggga aaggggagag agtagctaag agagagtttc
     1381 gccgaatggg aaaaaagatc ctcacgtcta gcgtgctaga gagggttgaa atagatcaca
     1441 ctgtcgttga cctttttgca gtacacgaag agtatcgaat cccattgggc cgaccttggc
     1501 ttactcaatt ggttgattgt tacagtaaag ctgtaatcgg tttttattta ggtttcgagc
     1561 ctcctagcta tgtgtcggtt tcccttgcac ttaagaatgc aatacaacgc aaagatgact
     1621 taatctcctc gtatgaatcg atcgagaatg aatggctatg ttatggcatc ccagacctac
     1681 tcgtaactga taatggtaaa gagtttttgt cgaaagcatt tgatcaagca tgtgaatcac
     1741 tattgatcaa tgtgcatcaa aataaagttg agacgcccga caacaaacct catgttgaac
     1801 gtaactacgg gactattaat acttctctgt tagacgattt acctgggaaa tccttcagcc
     1861 agtaccttca aagagaaggg tacgactctg tgggagaagc tacccttaca ctcaatgaga
     1921 ttagagaaat ttacttaatt tggttggtgg atatttatca taaaaaaccc aatcagagag
     1981 gcactaattg tcctaatgtt gcgtggaaaa agggttgtca agaatgggaa ccagaggagt
     2041 tctctggttc taaagacgaa ttagacttta aatttgctat tgttgattac aaacaactta
     2101 ctaaagtagg gataactgtc tacaaagaac tgagttatag caatgaccgt ttagctgaat
     2161 atagagggaa gaaaggaaac cataaagttc agttcaagta taaccctgag tgtatggcag
     2221 ttatttgggt gttggatgag gatatgaatg agtactttac agttaatgcg attgactacg
     2281 aatatgcaag tagagtatca ctttggcaac ataaatataa catgaaatat caagcagaac
     2341 taaattcagc agaatatgat gaggacaagg aaattgatgc agaaataaaa attgaagaaa
     2401 tcgcagatcg ttcaattgtt aagactaaca aaatcagagc tcggaggcgt ggcgctaggc
     2461 atcaagagaa tagcgcaagg gctaagtcaa tcagtaatgc gaacccggcc tcgatacaaa
     2521 aacatgaaga tgaaatcgtt agtgcagata atgacgattg ggatattgat tatgtctgag
     2581 aaatcgtcaa atgagtgaaa cgcgtgaggc tcgtatatca agagctaaaa gggcatttgt
     2641 atccacaccg agcgttagga aaatcttgag ttacatggat agatgtagag atctatcaga
     2701 cctagagtct gagcctacat gcatgatggt ctatggtgct tcgggtgtag gtaaaacgac
     2761 cgtcatcaag aaatacttaa atcaaaacag aagagagtcc gaagccgggg gcgatataat
     2821 accggttttg catattgagt tgccagacaa tgcgaagcca gtagatgcag caagggaatt
     2881 gctggttgaa atgggtgacc cgctagcact ttatgaaact gacttagcta gattgacgaa
     2941 aagactgact gaattaatcc ctgcggtcgg cgtgaagctg attattatcg atgagttcca
     3001 acatttggtg gaagaaaggt caaatcgggt tcttacccaa gtaggtaatt ggctaaaaat
     3061 gatacttaac aaaacgaaat gtccaattgt tatatttggt atgccatact caaaagttgt
     3121 actgcaagca aactcgcaac ttcacgggcg attttccatt caggttgaac tgcgcccctt
     3181 tagctaccag ggaggtagag gtgtatttaa aacttttttg gaataccttg ataaagccct
     3241 accttttgaa aaacaggctg gcttagccaa cgaaagtttg cagaaaaaat tgtatgcatt
     3301 ctctcaggga aacatgcgtt cgttgagaaa ccttatttat caagcatcta tcgaagcaat
     3361 tgataatcag catgagacga taaccgaaga agatttcgtt tttgcatcga agttgacatc
     3421 gggcgataaa cccaactcat ggaaaaatcc ttttgaggag ggtgttgagg taacagaaga
     3481 tatgttacga ccgccaccaa aagatattgg ttgggaggac tatttgagac attcaacccc
     3541 gagagtgagt aaaccaggta gaaataaaaa ctttttcgaa taacctaggc tgctggaagg
     3601 agatatacat atgtttttgc aaagacctaa accttacagc gatgaaagtt tagaaagttt
     3661 ctttatccga gtggctaaca aaaatggcta cggtgatgtc catcgcttcc tagaagccac
     3721 taaacgattc cttcaagaca ttgaccataa tggctatcaa acctttccga ctgatataac
     3781 tcggataaac ccatactcag ctaaaaacag ttccagcgca cgaactgcgt cattcctgaa
     3841 acttgcacaa ttgacattta atgaaccgcc agagctactt gggttggcaa ttaacagaac
     3901 aaacatgaaa tactcgccgt caactagcgc ggttgttaga ggtgcagaag tctttcctcg
     3961 cagtttacta cggacgcact ccatcccctg ctgtcctttg tgtctgcgag aaaatggcta
     4021 cgcctcctac ctttggcact ttcaggggta cgaatactgc cacagccata acgtaccttt
     4081 aattaccact tgtagctgtg gtaaggagtt tgactaccga gtatctgggt taaagggcat
     4141 ttgctgcaaa tgcaaggagc ctatcacctt aaccagcagg gagaacggtc atgaggcagc
     4201 gtgtactgtt tcaaactggc ttgctggcca tgaatctaaa cctctgccaa atcttcctaa
     4261 aagctaccga tggggtttag ttcattggtg gatgggtatt aaagatagcg agttcgatca
     4321 cttttcgttc gttcaatttt tctcaaactg gccaaggtca ttccactcga taatcgaaga
     4381 tgaagtagag ttcaaccttg agcatgctgt tgtcagcacg tctgaattac gactaaaaga
     4441 tcttcttggt cgattgtttt tcggttcaat tcggttacct gagcggaatc ttcaacacaa
     4501 tatcatcctt ggtgagcttc tctgctattt agaaaatcgt ttatggcaag acaagggatt
     4561 aatcgccaac ctcaaaatga acgcgttaga ggcgactgta atgttaaatt gtagcctcga
     4621 tcagattgca tcaatggttg aacaacgcat cttgaagcca aatcgaaaaa gcaagcccaa
     4681 cagccctctt gatgttaccg attatctatt tcatttcggc gatattttct gtctttggtt
     4741 agctgagttc caaagcgatg agtttaaccg ttcgttttat gtgtcgaggt ggtaaatgca
     4801 aactctgaaa gaactaatcg catccaatcc tgacgactta acaactgaac ttaagagagc
     4861 atttcgtcca ctcacaccgc atattgcaat tgatggtaat gaacttgacg cactgacgat
     4921 attagtcaat ttaaccgata agactgatga tcagaaagac ctgctcgatc gagccaaatg
     4981 caagcaaaaa cttcgagatg aaaaatggtg ggctagctgc ataaattgcg ttaattacag
     5041 acaaagccat aacccaaaat tcccggatat acgttctgaa ggcgtgattc gaacccaagc
     5101 cctgggtgaa ttaccgagtt tcctactctc gtcctcaaaa atcccaccat accattggtc
     5161 atatagccat gattcaaaat acgtcaacaa aagcgcattc ctcaccaatg agttttgttg
     5221 ggatggtgag atctcatgtt tgggtgagct tcttaaagat gcagatcacc cactttggaa
     5281 tactttgaaa aagttaggtt gttctcaaaa aacttgcaaa gcaatggcaa aacaactagc
     5341 tgatattact ctcacgacta tcaatgtcac actcgcacca aattacctga ctcaaatatc
     5401 tctccccgat agtgatacat cctacatttc actctctcct gttgcatcgc tatcgatgca
     5461 aagccacttt catcagaggc tccaagatga aaatcggcat agcgcgataa cgcggtttag
     5521 ccgaactacc aacatggggg tcactgcgat gacatgtggc ggcgcattta gaatgttaaa
     5581 gtctggcgct aagttttcca gcccccctca tcaccgatta aacagtaaac gaagttggtt
     5641 gacgtcagag catgttcagt cattaaaaca gtaccagcgc ctcaacaaaa gcctcatacc
     5701 tgaaaactct cggattgcac tccgtagaaa atacaaaatc gagcttcaaa atatggtcag
     5761 atcttggttt gcaatgcaag accataccct tgattcgaac atacttatcc aacatttgaa
     5821 tcatgaccta tcttacttag gagccacaaa acgttttgca tacgatccag cgatgaccaa
     5881 gctctttact gagcttttga aacgagagtt atcaaattca atcaataatg gtgagcaaca
     5941 cactaatgga tcgtttttag tcctaccgaa tatcagggtt tgtggcgcaa cagctttaag
     6001 ctccccggta acggtgggga ttccatcact tacagctttc tttggcttcg ttcacgcatt
     6061 tgaacggaat ataaatcgca ccacctcatc gtttcgtgtt gaatcctttg cgatatgcgt
     6121 ccatcaacta catgtcgaga agcgaggttt gacagcagag tttgtggaaa aaggcgacgg
     6181 gactatatcc gctcccgcga cccgggatga ctggcagtgt gatgtcgtat ttagccttat
     6241 tttgaacacc aactttgctc aacatattga ccaagatacg ttagttacat cactaccaaa
     6301 gcgattggct cggggttcag caaaaattgc gattgatgac tttaaacata tcaactcatt
     6361 ctcgacatta gaaacagcga tcgaatctct gccaatagaa gctggtaggt ggttatcact
     6421 ttacgcacag tcaaacaata atctaagtga tctattagca gccatgacag aggaccatca
     6481 gctcatggca agctgcgtcg gttaccactt gttagaagag cccaaagata aaccaaactc
     6541 cctcagaggt tacaaacacg ctatcgccga gtgcatcatt ggactcatta actcaatcac
     6601 ctttagctca gagactgatc ccaacacaat cttttggtcg ctaaagaact atcaaaacta
     6661 cctagtggta cagccaagga gtatcaacga tgaaactacc gacaaatcta gcctatgagc
     6721 gctctatcga cccatcagat gtctgttttt ttgtcgtctg gcccgatgat agaaaaacac
     6781 ctttaaccta caattctcgt actctgctcg ggcaaatgga agcggcatca ttagcctatg
     6841 atgtctcagg tcaaccaata aaaagtgcca ccgctgaggc gttagctcaa gggaaccctc
     6901 atcaagttga tttctgccac gttccatacg gtgcgagtca tattgaatgc agtttctccg
     6961 tctcgttttc ttctgaacta cgtcaaccat ataagtgtaa ctcaagcaaa gttaaacaaa
     7021 cgctagtgca attagtcgag ctctacgaaa cgaaaatcgg ctggactgag ctagcaaccc
     7081 gatatttgat gaacatttgc aacggtaaat ggctgtggaa aaatacccgt aaagcctatt
     7141 gctggaacat tgtacttaca ccttggcctt ggaacgggga aaaggttgga tttgaagata
     7201 ttcgtactaa ctacacctca cggcaagact ttaaaaataa taaaaattgg tctgctatag
     7261 ttgaaatgat caaaaccgca ttttctagta ctgatgggct ggcgatattt gaagtcaggg
     7321 ccaccttgca cttgccaacg aatgctatgg tgcggccaag ccaagttttc acagaaaaag
     7381 aaagtggcag taaaagtaaa tctaaaactc aaaacagtcg agtttttcag agtacaacta
     7441 ttgatggtga acgatcgcca atactagggg cctttaaaac gggagcagct attgcaacca
     7501 ttgacgactg gtatcctgaa gccactgagc cactaagggt cggacggttt ggggttcatc
     7561 gcgaagatgt cacttgctac cgtcatccgt ctaccggaaa agattttttc tcgatattac
     7621 aacaagcaga gcactatatt gaagtgttga gcgccaacaa aactcccgct caagaaacta
     7681 tcaacgacat gcacttttta atggctaacc tgattaaggg tgggatgttc cagcataaag
     7741 gagactgact gtgaaatggt attataagac aatcaccttt ctgccagagt tgtgcaacaa
     7801 cgagtcactg gctgcaaagt gtctccgcgt tctgcatgga tttaactatc agtatgagac
     7861 acgaaatatc ggcgtttcat ttccgctttg gtgtgatgca acggttggaa aaaagatttc
     7921 atttgtcagc aagaacaaga tagaactcga cttactactt aaacaacact atttcgtcca
     7981 aatggaacaa cttcaatact ttcatatatc caacactgtt ctcgtcccag aagattgtac
     8041 atacgtttcc tttagacgct gtcaatctat agataagctc acagcagcag ggctggcaag
     8101 gaaaatcaga cgcctggaga aacgtgctct atctagaggc gagcaatttg acccatcatc
     8161 ttttgctcaa aaagagcata ctgcaatagc gcactaccac tcacttgggg agtccagcaa
     8221 acagacgaac cgcaactttc gactcaatat caggatgctc tcggagcaac cccgtgaggg
     8281 gaactcgatt tttagtagct atggcttatc aaattcagaa aactcgtttc agcctgtacc
     8341 cttaatctga cgatatacca tgggtgaact gccgagtagg tagctgataa cgagacctct
     8401 gtcttgtcag ctagggtggt ctcgtgaact gccgagtagg tagctgataa cggatccgaa
     8461 ttcgctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     8521 ttgaggggtt ttttgctgaa acctcaggca tttgttgttg atacaaccat aaaatgataa
     8581 ttacacccat aaattgataa ttatcacacc cataaattga tattgcctct tcatggtcta
     8641 aacttcagta agtttacgac attttcctcg aggtcatttc ataacttcgt ataatgtatg
     8701 ctatacgaag ttatcttact gcagtagttt tgctgaaata ctcgattcac aaaaatatca
     8761 acttatggtt gttttgtgag atatcaatat atggttgttt tgtggttaag ttgctgatta
     8821 taaataatta ttaaatatca ctttatggtt gcatcaacag gtaccattat tcttaaatta
     8881 ttcaattaga taataattga ataatttaac gatttccctt tcatatttga tattgattag
     8941 ctcaccaatc aatacaattc gccgtcgttc aatgagaatg gataagaggc tcgtgggatt
     9001 gacgtgaggg ggcagggatg gctatatttc tgggagcgaa ctccgggcga tatcactagt
     9061 tgtagggagg gatccatggc tcgtgaagcg gttatcgccg aagtatcaac tcaactatca
     9121 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     9181 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     9241 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     9301 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     9361 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     9421 aatgacattc ttgcaggtat cttcgagcca gccacaatcg acattgatct ggctatcttg
     9481 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     9541 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     9601 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     9661 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     9721 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     9781 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     9841 ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg
     9901 ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag
     9961 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
    10021 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga
    10081 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10141 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10201 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10261 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10321 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10381 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10441 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10501 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10561 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10621 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10681 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10741 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10801 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10861 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
    10921 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10981 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
    11041 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
    11101 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
    11161 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
    11221 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
    11281 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
    11341 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
    11401 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
    11461 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
    11521 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagatacct
    11581 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
    11641 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
    11701 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
    11761 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
    11821 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
    11881 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
    11941 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
    12001 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
    12061 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
    12121 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
    12181 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
    12241 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
    12301 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
    12361 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
    12421 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
    12481 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
    12541 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
    12601 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
    12661 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg
    12721 gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc
    12781 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat
    12841 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt
    12901 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt
    12961 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc
    13021 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga
    13081 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa
    13141 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg
    13201 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg
    13261 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc
    13321 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg
    13381 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag
    13441 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt
    13501 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt
    13561 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg
    13621 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg
    13681 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct
    13741 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg
    13801 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt
    13861 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc
    13921 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc
    13981 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc
    14041 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc
    14101 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg
    14161 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca
    14221 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc
    14281 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg
    14341 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg
    14401 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc
    14461 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg
    14521 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg
    14581 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac
    14641 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg
    14701 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt
    14761 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc
    14821 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt
    14881 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
    14941 atcaaagtac tttaaagtac tttaaagtac tttaaagtac tttgatcccg aggggaaccc
    15001 tgtggttggc atgcggctca ccttcgggtg ggcctttctg cggagagcgt tcaccgacaa
    15061 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgagatcc
    15121 ttaa
//
