LOCUS       sequence-368899-       17827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty pVS1-based INTEGRATE vector with mCherry cargo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17827)
  AUTHORS   Aliu E, Lee K, Wang K
  TITLE     CRISPR RNA-guided integrase enables high-efficiency targeted genome 
            engineering in Agrobacterium tumefaciens.
  JOURNAL   Plant Biotechnol J. 2022 Jun 11. doi: 10.1111/pbi.13872.
  PUBMED    35690588
REFERENCE   2  (bases 1 to 17827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2022 Jun 11. doi: 10.1111/pbi.13872."
COMMENT     Sequence Label: pEA186
FEATURES             Location/Qualifiers
     source          1..17827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(724..742)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      738..785
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        931..965
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     RBS             975..986
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             993..1697
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMEELY"
     primer_bind     1406..1425
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1722..1793
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1945..2390
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(2391..2477,2478..3812)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     CDS             4029..4823
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MAREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPA
                     KRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDL
                     FEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQ
                     PVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5069..5657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5558..5577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5811..5828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5843..5983
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5909..5928)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6069..6091
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6327..6521
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6587..7660)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8089..8718)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     terminator      9968..9995
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(9996..10067)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        10082..10116
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             12238..12249
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtgaaatggt attataagac aatcaccttt ctgccagagt tgtgcaacaa cgagtcactg
       61 gctgcaaagt gtctccgcgt tctgcatgga tttaactatc agtatgagac acgaaatatc
      121 ggcgtttcat ttccgctttg gtgtgatgca acggttggaa aaaagatttc atttgtcagc
      181 aagaacaaga tagaactcga cttactactt aaacaacact atttcgtcca aatggaacaa
      241 cttcaatact ttcatatatc caacactgtt ctcgtcccag aagattgtac atacgtttcc
      301 tttagacgct gtcaatctat agataagctc acagcagcag ggctggcaag gaaaatcaga
      361 cgcctggaga aacgtgctct atctagaggc gagcaatttg acccatcatc ttttgctcaa
      421 aaagagcata ctgcaatagc gcactaccac tcacttgggg agtccagcaa acagacgaac
      481 cgcaactttc gactcaatat caggatgctc tcggagcaac cccgtgaggg gaactcgatt
      541 tttagtagct atggcttatc aaattcagaa aactcgtttc agcctgtacc cttaatctga
      601 cgatatacca tgggtgaact gccgagtagg tagctgataa cgagacctct gtcttgtcag
      661 ctagggtggt ctcgtgaact gccgagtagg tagctgataa cggatccgaa ttcgctgctg
      721 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      781 ttttgctgaa acctcaggca tttgttgttg atacaaccat aaaatgataa ttacacccat
      841 aaattgataa ttatcacacc cataaattga tattgcctct tcatggtcta aacttcagta
      901 agtttacgac attttcctcg aggtcatttc tttacggcta gctcagccct aggtattatg
      961 ctagcgaatt cattaaagag gagaaaacgc gtatggtgag caagggcgag gaggataaca
     1021 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1081 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1141 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1201 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     1261 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     1321 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     1381 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     1441 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     1501 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     1561 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     1621 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     1681 gcatggagga gctgtacaga tcttaaaagc tttaaggatc tcaaataaaa cgaaaggctc
     1741 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctccttactg
     1801 cagtagtttt gctgaaatac tcgattcaca aaaatatcaa cttatggttg ttttgtgaga
     1861 tatcaatata tggttgtttt gtggttaagt tgctgattat aaataattat taaatatcac
     1921 tttatggttg catcaacagg tacccacata tacctgccgt tcactattat ttagtgaaat
     1981 gagatattat gatattttct gaattgtgat taaaaaggca actttatgcc catgcaacag
     2041 aaactataaa aaatacagag aatgaaaaga aacagataga ttttttagtt ctttaggccc
     2101 gtagtctgca aatcctttta tgattttcta tcaaacaaaa gaggaaaata gaccagttgc
     2161 aatccaaacg agagtctaat agaatgaggt cgaaaagtaa atcgcgcggg tttgttactg
     2221 ataaagcagg caagacctaa aatgtgtaaa gggcaaagtg tatactttgg cgtcacccct
     2281 tacatatttt aggtcttttt ttattgtgcg taactaactt gccatcttca aacaggaggg
     2341 ctggaagaag cagaccgcta acacagtaca taaaaaagga gacatgaacg atgaacatca
     2401 aaaagtttgc aaaacaagca acagtattaa cctttactac cgcactgctg gcaggaggcg
     2461 caactcaagc gtttgcgaaa gaaacgaacc aaaagccata taaggaaaca tacggcattt
     2521 cccatattac acgccatgat atgctgcaaa tccctgaaca gcaaaaaaat gaaaaatatc
     2581 aagtttctga atttgattcg tccacaatta aaaatatctc ttctgcaaaa ggcctggacg
     2641 tttgggacag ctggccatta caaaacgctg acggcactgt cgcaaactat cacggctacc
     2701 acatcgtctt tgcattagcc ggagatccta aaaatgcgga tgacacatcg atttacatgt
     2761 tctatcaaaa agtcggcgaa acttctattg acagctggaa aaacgctggc cgcgtcttta
     2821 aagacagcga caaattcgat gcaaatgatt ctatcctaaa agaccaaaca caagaatggt
     2881 caggttcagc cacatttaca tctgacggaa aaatccgttt attctacact gatttctccg
     2941 gtaaacatta cggcaaacaa acactgacaa ctgcacaagt taacgtatca gcatcagaca
     3001 gctctttgaa catcaacggt gtagaggatt ataaatcaat ctttgacggt gacggaaaaa
     3061 cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta cagctcaggc gacaaccata
     3121 cgctgagaga tcctcactac gtagaagata aaggccacaa atacttagta tttgaagcaa
     3181 acactggaac tgaagatggc taccaaggcg aagaatcttt atttaacaaa gcatactatg
     3241 gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact tctgcaaagc gataaaaaac
     3301 gcacggctga gttagcaaac ggcgctctcg gtatgattga gctaaacgat gattacacac
     3361 tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt aacagatgaa attgaacgcg
     3421 cgaacgtctt taaaatgaac ggcaaatggt acctgttcac tgactcccgc ggatcaaaaa
     3481 tgacgattga cggcattacg tctaacgata tttacatgct tggttatgtt tctaattctt
     3541 taactggccc atacaagccg ctgaacaaaa ctggccttgt gttaaaaatg gatcttgatc
     3601 ctaacgatgt aacctttact tactcacact tcgctgtacc tcaagcgaaa ggaaacaatg
     3661 tcgtgattac aagctatatg acaaacagag gattctacgc agacaaacaa tcaacgtttg
     3721 cgccgagctt cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc
     3781 ttgaacaagg acaattaaca gttaacaaat aaggtaccat tattcttaaa ttattcaatt
     3841 agataataat tgaataattt aacgatttcc ctttcatatt tgatattgat tagctcacca
     3901 atcaatacaa ttcgccgtcg ttcaatgaga atggataaga ggctcgtggg attgacgtga
     3961 gggggcaggg atggctatat ttctgggagc gaactccggg cgatatcact agttgtaggg
     4021 agggatccat ggctcgtgaa gcggttatcg ccgaagtatc aactcaacta tcagaggtag
     4081 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     4141 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     4201 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     4261 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     4321 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     4381 ttcttgcagg tatcttcgag ccagccacaa tcgacattga tctggctatc ttgctgacaa
     4441 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     4501 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     4561 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     4621 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     4681 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     4741 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     4801 tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc
     4861 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     4921 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     4981 gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     5221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5821 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5881 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5941 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     6001 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     6061 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     6121 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     6181 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     6241 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     6301 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     6361 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     6421 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     6481 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagata ccttttcgac
     6541 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     6601 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     6661 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     6721 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     6781 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     6841 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     6901 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     6961 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     7021 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     7081 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     7141 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     7201 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     7261 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     7321 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     7381 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     7441 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     7501 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     7561 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     7621 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
     7681 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc
     7741 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
     7801 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
     7861 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
     7921 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
     7981 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
     8041 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
     8101 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
     8161 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
     8221 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
     8281 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
     8341 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
     8401 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
     8461 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
     8521 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
     8581 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
     8641 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
     8701 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
     8761 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
     8821 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
     8881 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
     8941 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
     9001 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
     9061 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
     9121 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
     9181 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
     9241 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
     9301 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
     9361 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
     9421 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
     9481 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
     9541 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
     9601 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
     9661 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
     9721 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
     9781 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
     9841 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
     9901 tactttaaag tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt
     9961 ggcatgcggc tcaccttcgg gtgggccttt ctgcggagag cgttcaccga caaacaacag
    10021 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgaga tccttaaagc
    10081 tttgacagct agctcagtcc taggtataat actagcgtcg acaggatccg aattcgagct
    10141 cggtataagg agatatacca tggcgacaag tttacctacg ccctcagcaa ttacgacttc
    10201 ggcgttagag tatgcattcc atactcccgc tcgcaatcta acgaaatctc gcggaaaaaa
    10261 cattcatcgt tatgtcagtg taaagatgag taagaggatt acggtagaat ctactctaga
    10321 gtgtgatgcc tgctatcact ttgattttga gccaagtatt gttcgctttt gcgctcaacc
    10381 gattcgattt ttatattatc tcaatggtca gtctcactcc tatgttcctg actttctagt
    10441 tcaatttgat accaacgagt ttgttctata tgaagtaaag tcagcttatg ctaagaacaa
    10501 acctgatttt gatgttgaat gggaggcgaa agtaaaagca gcaactgaac tagggctaga
    10561 attggagctt gttgaagaga gtgatattag ggatacggtt gtattaaata atcttaagcg
    10621 catgcatcgt tatgcttcga aagatgagct gaataacgta cataactctc tcttaaaaat
    10681 aataaagtac aatggcgccc aatctgcaag atgcttggga gaacagttgg gtttaaaagg
    10741 ccgaactgtt ttaccaattt tgtgcgattt gctgtcaagg tgtttactcg atacacgttt
    10801 ggataagcct ctatctcttg aatctcgatt tgagttggcc agttatggct aagaaagggt
    10861 tctcaagttt ccatagaaaa gcagtctctt cccaagatac gcttgaatcg atagagcttg
    10921 tctctagcgc taattgtcta gaaagtgtta cgtatcaaga tatatcagca tttcccgaaa
    10981 caattgcggt agagattaat ttccgattaa gcattcttcg ctttttagcg cgaaagtgcg
    11041 aaaccattgt ggccaaatca attgaaccac atcgtgtaga gctacagcaa aactatagta
    11101 gaaaaatacc cagtgcaata acgatatatc gatggtggct tgcttttcga aaatcagact
    11161 acaaccccat tagcttagca cctaatatca aggatagagg taatagagag acaaaagtgt
    11221 caacagttgt tgattctatt atggaacagg cagttgaaag agttatatct ggacgaaaag
    11281 tcaatgttag ctctgcatat aaacgtgttc gacgaaaagt tcgtcaatac aatctaactc
    11341 atggaacgaa atacacgtat cctaagtacg aatctgtaag aaagcgagta aaaaagaaaa
    11401 ccccatttga gttattagcc gcagggaaag gggagagagt agctaagaga gagtttcgcc
    11461 gaatgggaaa aaagatcctc acgtctagcg tgctagagag ggttgaaata gatcacactg
    11521 tcgttgacct ttttgcagta cacgaagagt atcgaatccc attgggccga ccttggctta
    11581 ctcaattggt tgattgttac agtaaagctg taatcggttt ttatttaggt ttcgagcctc
    11641 ctagctatgt gtcggtttcc cttgcactta agaatgcaat acaacgcaaa gatgacttaa
    11701 tctcctcgta tgaatcgatc gagaatgaat ggctatgtta tggcatccca gacctactcg
    11761 taactgataa tggtaaagag tttttgtcga aagcatttga tcaagcatgt gaatcactat
    11821 tgatcaatgt gcatcaaaat aaagttgaga cgcccgacaa caaacctcat gttgaacgta
    11881 actacgggac tattaatact tctctgttag acgatttacc tgggaaatcc ttcagccagt
    11941 accttcaaag agaagggtac gactctgtgg gagaagctac ccttacactc aatgagatta
    12001 gagaaattta cttaatttgg ttggtggata tttatcataa aaaacccaat cagagaggca
    12061 ctaattgtcc taatgttgcg tggaaaaagg gttgtcaaga atgggaacca gaggagttct
    12121 ctggttctaa agacgaatta gactttaaat ttgctattgt tgattacaaa caacttacta
    12181 aagtagggat aactgtctac aaagaactga gttatagcaa tgaccgttta gctgaatata
    12241 gagggaagaa aggaaaccat aaagttcagt tcaagtataa ccctgagtgt atggcagtta
    12301 tttgggtgtt ggatgaggat atgaatgagt actttacagt taatgcgatt gactacgaat
    12361 atgcaagtag agtatcactt tggcaacata aatataacat gaaatatcaa gcagaactaa
    12421 attcagcaga atatgatgag gacaaggaaa ttgatgcaga aataaaaatt gaagaaatcg
    12481 cagatcgttc aattgttaag actaacaaaa tcagagctcg gaggcgtggc gctaggcatc
    12541 aagagaatag cgcaagggct aagtcaatca gtaatgcgaa cccggcctcg atacaaaaac
    12601 atgaagatga aatcgttagt gcagataatg acgattggga tattgattat gtctgagaaa
    12661 tcgtcaaatg agtgaaacgc gtgaggctcg tatatcaaga gctaaaaggg catttgtatc
    12721 cacaccgagc gttaggaaaa tcttgagtta catggataga tgtagagatc tatcagacct
    12781 agagtctgag cctacatgca tgatggtcta tggtgcttcg ggtgtaggta aaacgaccgt
    12841 catcaagaaa tacttaaatc aaaacagaag agagtccgaa gccgggggcg atataatacc
    12901 ggttttgcat attgagttgc cagacaatgc gaagccagta gatgcagcaa gggaattgct
    12961 ggttgaaatg ggtgacccgc tagcacttta tgaaactgac ttagctagat tgacgaaaag
    13021 actgactgaa ttaatccctg cggtcggcgt gaagctgatt attatcgatg agttccaaca
    13081 tttggtggaa gaaaggtcaa atcgggttct tacccaagta ggtaattggc taaaaatgat
    13141 acttaacaaa acgaaatgtc caattgttat atttggtatg ccatactcaa aagttgtact
    13201 gcaagcaaac tcgcaacttc acgggcgatt ttccattcag gttgaactgc gcccctttag
    13261 ctaccaggga ggtagaggtg tatttaaaac ttttttggaa taccttgata aagccctacc
    13321 ttttgaaaaa caggctggct tagccaacga aagtttgcag aaaaaattgt atgcattctc
    13381 tcagggaaac atgcgttcgt tgagaaacct tatttatcaa gcatctatcg aagcaattga
    13441 taatcagcat gagacgataa ccgaagaaga tttcgttttt gcatcgaagt tgacatcggg
    13501 cgataaaccc aactcatgga aaaatccttt tgaggagggt gttgaggtaa cagaagatat
    13561 gttacgaccg ccaccaaaag atattggttg ggaggactat ttgagacatt caaccccgag
    13621 agtgagtaaa ccaggtagaa ataaaaactt tttcgaataa cctaggctgc tggaaggaga
    13681 tatacatatg tttttgcaaa gacctaaacc ttacagcgat gaaagtttag aaagtttctt
    13741 tatccgagtg gctaacaaaa atggctacgg tgatgtccat cgcttcctag aagccactaa
    13801 acgattcctt caagacattg accataatgg ctatcaaacc tttccgactg atataactcg
    13861 gataaaccca tactcagcta aaaacagttc cagcgcacga actgcgtcat tcctgaaact
    13921 tgcacaattg acatttaatg aaccgccaga gctacttggg ttggcaatta acagaacaaa
    13981 catgaaatac tcgccgtcaa ctagcgcggt tgttagaggt gcagaagtct ttcctcgcag
    14041 tttactacgg acgcactcca tcccctgctg tcctttgtgt ctgcgagaaa atggctacgc
    14101 ctcctacctt tggcactttc aggggtacga atactgccac agccataacg tacctttaat
    14161 taccacttgt agctgtggta aggagtttga ctaccgagta tctgggttaa agggcatttg
    14221 ctgcaaatgc aaggagccta tcaccttaac cagcagggag aacggtcatg aggcagcgtg
    14281 tactgtttca aactggcttg ctggccatga atctaaacct ctgccaaatc ttcctaaaag
    14341 ctaccgatgg ggtttagttc attggtggat gggtattaaa gatagcgagt tcgatcactt
    14401 ttcgttcgtt caatttttct caaactggcc aaggtcattc cactcgataa tcgaagatga
    14461 agtagagttc aaccttgagc atgctgttgt cagcacgtct gaattacgac taaaagatct
    14521 tcttggtcga ttgtttttcg gttcaattcg gttacctgag cggaatcttc aacacaatat
    14581 catccttggt gagcttctct gctatttaga aaatcgttta tggcaagaca agggattaat
    14641 cgccaacctc aaaatgaacg cgttagaggc gactgtaatg ttaaattgta gcctcgatca
    14701 gattgcatca atggttgaac aacgcatctt gaagccaaat cgaaaaagca agcccaacag
    14761 ccctcttgat gttaccgatt atctatttca tttcggcgat attttctgtc tttggttagc
    14821 tgagttccaa agcgatgagt ttaaccgttc gttttatgtg tcgaggtggt aaatgcaaac
    14881 tctgaaagaa ctaatcgcat ccaatcctga cgacttaaca actgaactta agagagcatt
    14941 tcgtccactc acaccgcata ttgcaattga tggtaatgaa cttgacgcac tgacgatatt
    15001 agtcaattta accgataaga ctgatgatca gaaagacctg ctcgatcgag ccaaatgcaa
    15061 gcaaaaactt cgagatgaaa aatggtgggc tagctgcata aattgcgtta attacagaca
    15121 aagccataac ccaaaattcc cggatatacg ttctgaaggc gtgattcgaa cccaagccct
    15181 gggtgaatta ccgagtttcc tactctcgtc ctcaaaaatc ccaccatacc attggtcata
    15241 tagccatgat tcaaaatacg tcaacaaaag cgcattcctc accaatgagt tttgttggga
    15301 tggtgagatc tcatgtttgg gtgagcttct taaagatgca gatcacccac tttggaatac
    15361 tttgaaaaag ttaggttgtt ctcaaaaaac ttgcaaagca atggcaaaac aactagctga
    15421 tattactctc acgactatca atgtcacact cgcaccaaat tacctgactc aaatatctct
    15481 ccccgatagt gatacatcct acatttcact ctctcctgtt gcatcgctat cgatgcaaag
    15541 ccactttcat cagaggctcc aagatgaaaa tcggcatagc gcgataacgc ggtttagccg
    15601 aactaccaac atgggggtca ctgcgatgac atgtggcggc gcatttagaa tgttaaagtc
    15661 tggcgctaag ttttccagcc cccctcatca ccgattaaac agtaaacgaa gttggttgac
    15721 gtcagagcat gttcagtcat taaaacagta ccagcgcctc aacaaaagcc tcatacctga
    15781 aaactctcgg attgcactcc gtagaaaata caaaatcgag cttcaaaata tggtcagatc
    15841 ttggtttgca atgcaagacc atacccttga ttcgaacata cttatccaac atttgaatca
    15901 tgacctatct tacttaggag ccacaaaacg ttttgcatac gatccagcga tgaccaagct
    15961 ctttactgag cttttgaaac gagagttatc aaattcaatc aataatggtg agcaacacac
    16021 taatggatcg tttttagtcc taccgaatat cagggtttgt ggcgcaacag ctttaagctc
    16081 cccggtaacg gtggggattc catcacttac agctttcttt ggcttcgttc acgcatttga
    16141 acggaatata aatcgcacca cctcatcgtt tcgtgttgaa tcctttgcga tatgcgtcca
    16201 tcaactacat gtcgagaagc gaggtttgac agcagagttt gtggaaaaag gcgacgggac
    16261 tatatccgct cccgcgaccc gggatgactg gcagtgtgat gtcgtattta gccttatttt
    16321 gaacaccaac tttgctcaac atattgacca agatacgtta gttacatcac taccaaagcg
    16381 attggctcgg ggttcagcaa aaattgcgat tgatgacttt aaacatatca actcattctc
    16441 gacattagaa acagcgatcg aatctctgcc aatagaagct ggtaggtggt tatcacttta
    16501 cgcacagtca aacaataatc taagtgatct attagcagcc atgacagagg accatcagct
    16561 catggcaagc tgcgtcggtt accacttgtt agaagagccc aaagataaac caaactccct
    16621 cagaggttac aaacacgcta tcgccgagtg catcattgga ctcattaact caatcacctt
    16681 tagctcagag actgatccca acacaatctt ttggtcgcta aagaactatc aaaactacct
    16741 agtggtacag ccaaggagta tcaacgatga aactaccgac aaatctagcc tatgagcgct
    16801 ctatcgaccc atcagatgtc tgtttttttg tcgtctggcc cgatgataga aaaacacctt
    16861 taacctacaa ttctcgtact ctgctcgggc aaatggaagc ggcatcatta gcctatgatg
    16921 tctcaggtca accaataaaa agtgccaccg ctgaggcgtt agctcaaggg aaccctcatc
    16981 aagttgattt ctgccacgtt ccatacggtg cgagtcatat tgaatgcagt ttctccgtct
    17041 cgttttcttc tgaactacgt caaccatata agtgtaactc aagcaaagtt aaacaaacgc
    17101 tagtgcaatt agtcgagctc tacgaaacga aaatcggctg gactgagcta gcaacccgat
    17161 atttgatgaa catttgcaac ggtaaatggc tgtggaaaaa tacccgtaaa gcctattgct
    17221 ggaacattgt acttacacct tggccttgga acggggaaaa ggttggattt gaagatattc
    17281 gtactaacta cacctcacgg caagacttta aaaataataa aaattggtct gctatagttg
    17341 aaatgatcaa aaccgcattt tctagtactg atgggctggc gatatttgaa gtcagggcca
    17401 ccttgcactt gccaacgaat gctatggtgc ggccaagcca agttttcaca gaaaaagaaa
    17461 gtggcagtaa aagtaaatct aaaactcaaa acagtcgagt ttttcagagt acaactattg
    17521 atggtgaacg atcgccaata ctaggggcct ttaaaacggg agcagctatt gcaaccattg
    17581 acgactggta tcctgaagcc actgagccac taagggtcgg acggtttggg gttcatcgcg
    17641 aagatgtcac ttgctaccgt catccgtcta ccggaaaaga ttttttctcg atattacaac
    17701 aagcagagca ctatattgaa gtgttgagcg ccaacaaaac tcccgctcaa gaaactatca
    17761 acgacatgca ctttttaatg gctaacctga ttaagggtgg gatgttccag cataaaggag
    17821 actgact
//
