LOCUS       sequence-368924-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABEmax-SpCas9-NGC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han
            R
  TITLE     Efficient precise in vivo base editing in adult dystrophic mice.
  JOURNAL   Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y.
  PUBMED    34140489
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-17"; volume: "12(1):3719. doi"; pages: " 
            10.1038/s41467-021-23996-y"
COMMENT     Sequence Label: pCMV_ABE‐NGC
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(510..1301,1302..1370))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1133..1152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1371..1475)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1554..1573)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1745..2124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2125..2328
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2299
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2370..2389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2370..2388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2438..2458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3647..7747
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKEYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7790..7810
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7819..7836
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7859..7876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7865..8089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8160..8176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8160..8176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8173..8195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8184..8200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8208..8214,8215..8232,8233..8238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8253..8274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8391..8408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8562..8811,1..339))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8642..8661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
       61 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      121 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      181 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      241 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      361 acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1381 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1441 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1501 gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc
     1561 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     1621 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     1681 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     1741 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     1801 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1861 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     1921 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     1981 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2041 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2101 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2161 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2221 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2281 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     2341 cgtcagatcc gctagagatc cgcggccgct aatacgactc actataggga gagccgccac
     2401 catgaaacgg acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc
     2461 tgaagtcgag tttagccacg agtattggat gaggcacgca ctgaccctgg caaagcgagc
     2521 atgggatgaa agagaagtcc ccgtgggcgc cgtgctggtg cacaacaata gagtgatcgg
     2581 agagggatgg aacaggccaa tcggccgcca cgaccctacc gcacacgcag agatcatggc
     2641 actgaggcag ggaggcctgg tcatgcagaa ttaccgcctg atcgatgcca ccctgtatgt
     2701 gacactggag ccatgcgtga tgtgcgcagg agcaatgatc cacagcagga tcggaagagt
     2761 ggtgttcgga gcacgggacg ccaagaccgg cgcagcaggc tccctgatgg atgtgctgca
     2821 ccaccccggc atgaaccacc gggtggagat cacagaggga atcctggcag acgagtgcgc
     2881 cgccctgctg agcgatttct ttagaatgcg gagacaggag atcaaggccc agaagaaggc
     2941 acagagctcc accgactctg gaggatctag cggaggatcc tctggaagcg agacaccagg
     3001 cacaagcgag tccgccacac cagagagctc cggcggctcc tccggaggat cctctgaggt
     3061 ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga
     3121 tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg
     3181 ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag
     3241 acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt
     3301 cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt
     3361 tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc
     3421 cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct
     3481 gctgtgctat ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag
     3541 ctccaccgac tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag
     3601 cgagagcgca acacctgaaa gcagcggggg cagcagcggg gggtcagaca agaagtacag
     3661 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     3721 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     3781 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
     3841 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     3901 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     3961 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     4021 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     4081 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     4141 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     4201 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     4261 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     4321 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
     4381 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     4441 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     4501 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     4561 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     4621 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     4681 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     4741 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     4801 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     4861 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     4921 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     4981 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     5041 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     5101 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     5161 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     5221 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     5281 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     5341 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     5401 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     5461 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     5521 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     5581 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     5641 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     5701 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     5761 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     5821 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     5881 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     5941 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     6001 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     6061 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     6121 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     6181 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa
     6241 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     6301 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     6361 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     6421 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     6481 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     6541 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     6601 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     6661 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     6721 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     6781 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     6841 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     6901 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     6961 caaagagtct atccggccca agaggaacag cgataagctg atcgccagaa agaaggactg
     7021 ggaccctaag aagtacggcg gcttcgtcag ccccaccgtg gcctattctg tgctggtggt
     7081 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     7141 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     7201 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     7261 ggaaaacggc cggaagagaa tgctggcctc tgcccgcttt ctgcagaagg gaaacgaact
     7321 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     7381 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     7441 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     7501 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     7561 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctcggg ccttcaagta
     7621 ctttgacacc accatcgacc ggaaggagta ccggagcacc aaagaggtgc tggacgccac
     7681 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     7741 aggtgactct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa
     7801 gaggaaagtc taaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     7861 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7921 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7981 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     8041 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     8101 gaaagaacca gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg
     8161 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8221 ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg
     8281 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8341 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8401 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8461 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8521 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8581 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8641 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8701 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8761 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg c
//
