LOCUS       sequence-369-exp        6820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, 
            Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W
  TITLE     Visualization of retroviral replication in living cells reveals 
            budding into multivesicular bodies.
  JOURNAL   Traffic 2003 Nov;4(11):785-801.
  PUBMED    14617360
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic"; 
            date: "2003-11"; volume: "4(11)"; pages: "785-801"
COMMENT     Sequence Label: MLV Gag-CFP
FEATURES             Location/Qualifiers
     source          1..6820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        461..664
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     615..635
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     661..685
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          799..1274
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1332..1748
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1678..1700
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1716..1732
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             2925..3635
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2964..2985)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3225..3244)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3572..3593
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3812..4205
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(4540..4558)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4626..4730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4731..4799,4800..5591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4949..4968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     6141..6160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatattgga gttccgcgtt acataactta cggtaaatgg
      181 cccgcctggc tgaccgccca acgacccccg ccccttgacg tcaataatga cgtatgttcc
      241 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      301 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      361 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      421 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tgtggcagta
      481 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      541 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      601 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      661 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      721 tagaagacac cgggaccgat ccagcctccc ctcgaagctt tacatgtggt accgagctcg
      781 gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc cccttctttt
      841 ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat attgaccaaa
      901 tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt
      961 gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat
     1021 gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggcaa
     1081 tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt
     1141 catattgcta atagcagcta caatccagct accattctgc ttttatttta tggttgggat
     1201 aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta
     1261 tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca
     1321 aagaattcat gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1381 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1441 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1501 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1561 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1621 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1681 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1741 taggcgccaa acctaaacct caagttcttt ctgacagtgg ggggccgctc atcgacctac
     1801 ttacagaaga ccccccgcct tatagggacc caagaccacc cccttccgac agggacggaa
     1861 atggtggaga agcgacccct gcgggagagg caccggaccc ctccccaatg gcatctcgcc
     1921 tacgtgggag acgggagccc cctgtggccg actccactac ctcgcaggca ttccccctcc
     1981 gcgcaggagg aaacggacag cttcaatact ggccgttctc ctcttctgac ctttacaact
     2041 ggaaaaataa taacccttct ttttctgaag atccaggtaa actgacagct ctgatcgagt
     2101 ctgttctcat cacccatcag cccacctggg acgactgtca gcagctgttg gggactctgc
     2161 tgaccggaga agaaaaacaa cgggtgctct tagaggctag aaaggcggtg cggggcgatg
     2221 atgggcgccc cactcaactg cccaatgaag tcgatgccgc ttttcccctc gagcgcccag
     2281 actgggatta caccacccag gcaggtagga accacctagt ccactatcgc cagttgctcc
     2341 tagcgggtct ccaaaacgcg ggcagaagcc ccaccaattt ggccaaggta aaaggaataa
     2401 cacaagggcc caatgagtct ccctcggcct tcctagagag acttaaggaa gcctatcgca
     2461 ggtacactcc ttatgaccct gaggacccag ggcaagaaac taatgtgtct atgtctttca
     2521 tttggcagtc tgccccagac attgggagaa agttagagag gttagaagat ttaaaaaaca
     2581 agacgcttgg agatttggtt agagaggcag aaaagatctt taataaacga gaaaccccgg
     2641 aagaaagaga ggaacgtatc aggagagaaa cagaggaaaa agaagaacgc cgtaggacag
     2701 aggatgagca gaaagagaaa gaaagagatc gtaggagaca tagagagatg agcaagctat
     2761 tggccactgt cgttagtgga cagaaacagg atagacaggg aggagaacga aggaggtccc
     2821 aactcgatcg cgaccagtgt gcctactgca aagaaaaggg gcactgggct aaagattgtc
     2881 ccaagaaacc acgaggacct cggggaccaa gaccgcaggc tagcagcaag ggcgaggagc
     2941 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3001 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3061 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     3121 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3181 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3241 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3301 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     3361 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     3421 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3481 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     3541 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3601 ccgggatcac tctcggcatg gacgagctgt acaaggctag cccgggggtg gaccatcctc
     3661 tagactgaca tggcgcgttc aacgctctca aaacccctta aaaataaggt taacccgcga
     3721 ggccccctaa gaattcaccc caccagtgca ggctgcctat cagaaagtgg tggctggtgt
     3781 ggctaatgcc ctggcccaca agtatcacta agctcgcttt cttgctgtcc aatttctatt
     3841 aaaggttcct ttgttcccta agtccaacta ctaaactggg ggatattatg aagggccttg
     3901 agcatctgga ttctgcctaa taaaaaacat ttattttcat tgcaatgatg tatttaaatt
     3961 atttctgaat attttactaa aaagggaatg tgggaggtca gtgcatttaa aacataaaga
     4021 aatgaagagc tagttcaaac cttgggaaaa tacactatat cttaaactcc atgaaagaag
     4081 gtgaggctgc aaacagctaa tgcacattgg caacagccct gatgcctatg ccttattcat
     4141 ccctcagaaa aggattcaag tagaggcttg atttggaggt taaagttttg ctatgctgta
     4201 ttttacatta cttattgttt tagctgtcct catgaatgtc ttttcactac ccatttgctt
     4261 atcctgcatc tctcagcctt gactccactc agttctcttg cttagagata ccacctttcc
     4321 cctgaagtgt tccttccatg ttttacggcg aagatggttt ctcctcgcct ggccactcag
     4381 ccttagttgt ctctgttgtc ttatagaggt ctacttgaag aaggaaaaac agggggcatg
     4441 gtttgactgt cctgtgagcc cttcttccct gcctccccca ctcacagtga cccggaatcc
     4501 ctcgacatgg cagtctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     4561 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4621 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     4981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     5281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5641 atttttaatt taaaaggatc taggtgaaga tccgtaatct gctgcttgca aacaaaaaaa
     5701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5761 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6241 aacgccagca acggatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     6301 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     6361 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     6421 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     6481 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atccccgtga cgatcagcgg
     6541 tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
     6601 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     6661 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcggggagc tttttgcaaa
     6721 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     6781 cctcggcctc tgcataaata aaaaaaatta gtcagccatg
//
