LOCUS       sequence-36900-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4120 (=HL 716 + pHL 1370)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(64..81)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      234..320
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      412..439
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    506..524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atggatgagc tctacaaata aaagctagct tggctgtttt ggcggatgag agaagatttt
       61 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
      121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
      181 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
      241 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      301 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      361 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
      421 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat
      481 tcaaatatgt atccgctcag gatcctccct atcagtgata gagattgaca tccctatcag
      541 tgatagagat actccgggaa cacatcagat ttcctggtgt aacgaatttt ttaagtgctt
      601 cttgcttaag caagtttcat cccgaccccc tcagggtcgg gattttttta ttgtgcattc
      661 aacgattcac ttcagagacg ctaagcttta atcgtacagg gtagtacaaa taaaaaaggc
      721 acgtcagatg acgtgccttt tttcttgtga gcagtgaatt ctcgagcacc gtcgttgttg
      781 acatttttat gcttggcggt tataatggat t
//
