LOCUS       sequence-369025-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABEmax-SpCas9-NGX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han
            R
  TITLE     Efficient precise in vivo base editing in adult dystrophic mice.
  JOURNAL   Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y.
  PUBMED    34140489
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-17"; volume: "12(1):3719. doi"; pages: " 
            10.1038/s41467-021-23996-y"
COMMENT     Sequence Label: pCMV‐ABEmaxNGX
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     512..531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(750..854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(933..952)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1124..1503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1504..1707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1658..1678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1749..1768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1749..1767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1817..1837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3026..7126
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7169..7189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7198..7215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7238..7255)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7244..7468
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7539..7555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7539..7555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7552..7574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7563..7579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7587..7593,7594..7611,7612..7617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7632..7653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7770..7787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7941..8529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8021..8040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8700..8811,1..680,681..749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
       61 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
      361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
      421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
      481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
      541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
      601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
      661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
      721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
      781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
      841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
      901 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
      961 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     1021 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     1081 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     1141 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1201 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1261 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1321 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1381 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1441 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1501 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1561 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1621 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1681 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc
     1741 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
     1801 aagcgagttc gagtcaccaa agaagaagcg gaaagtctct gaagtcgagt ttagccacga
     1861 gtattggatg aggcacgcac tgaccctggc aaagcgagca tgggatgaaa gagaagtccc
     1921 cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga gagggatgga acaggccaat
     1981 cggccgccac gaccctaccg cacacgcaga gatcatggca ctgaggcagg gaggcctggt
     2041 catgcagaat taccgcctga tcgatgccac cctgtatgtg acactggagc catgcgtgat
     2101 gtgcgcagga gcaatgatcc acagcaggat cggaagagtg gtgttcggag cacgggacgc
     2161 caagaccggc gcagcaggct ccctgatgga tgtgctgcac caccccggca tgaaccaccg
     2221 ggtggagatc acagagggaa tcctggcaga cgagtgcgcc gccctgctga gcgatttctt
     2281 tagaatgcgg agacaggaga tcaaggccca gaagaaggca cagagctcca ccgactctgg
     2341 aggatctagc ggaggatcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc
     2401 agagagctcc ggcggctcct ccggaggatc ctctgaggtg gagttttccc acgagtactg
     2461 gatgagacat gccctgaccc tggccaagag ggcacgcgat gagagggagg tgcctgtggg
     2521 agccgtgctg gtgctgaaca atagagtgat cggcgagggc tggaacagag ccatcggcct
     2581 gcacgaccca acagcccatg ccgaaattat ggccctgaga cagggcggcc tggtcatgca
     2641 gaactacaga ctgattgacg ccaccctgta cgtgacattc gagccttgcg tgatgtgcgc
     2701 cggcgccatg atccactcta ggatcggccg cgtggtgttt ggcgtgagga acgcaaaaac
     2761 cggcgccgca ggctccctga tggacgtgct gcactacccc ggcatgaatc accgcgtcga
     2821 aattaccgag ggaatcctgg cagatgaatg tgccgccctg ctgtgctatt tctttcggat
     2881 gcctagacag gtgttcaatg ctcagaagaa ggcccagagc tccaccgact ccggaggatc
     2941 tagcggaggc tcctctggct ctgagacacc tggcacaagc gagagcgcaa cacctgaaag
     3001 cagcgggggc agcagcgggg ggtcagacaa gaagtacagc atcggcctgg ccatcggcac
     3061 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     3121 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     3181 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     3241 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     3301 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     3361 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     3421 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     3481 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     3541 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     3601 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     3661 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     3721 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     3781 gagcaacttc gacctggccg aggataccaa actgcagctg agcaaggaca cctacgacga
     3841 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     3901 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     3961 caaggccccc ctgagcgcct ctatgatcaa gctgtacgac gagcaccacc aggacctgac
     4021 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     4081 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     4141 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     4201 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcatca tcccccacca
     4261 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     4321 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     4381 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     4441 caccccctgg aacttcgaga aggtggtgga caagggcgct tccgcccaga gcttcatcga
     4501 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     4561 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     4621 aatgagaaag cccgccttcc tgagcggcga ccagaaaaag gccatcgtgg acctgctgtt
     4681 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     4741 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     4801 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     4861 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     4921 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     4981 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     5041 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     5101 catccagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     5161 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     5221 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     5281 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     5341 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     5401 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     5461 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     5521 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     5581 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     5641 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     5701 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     5761 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     5821 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     5881 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     5941 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     6001 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     6061 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     6121 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     6181 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     6241 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     6301 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tccggcccaa
     6361 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     6421 cttcgtcagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     6481 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     6541 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     6601 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     6661 gctggcctct gcccgctttc tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     6721 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     6781 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     6841 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     6901 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     6961 taccctgacc aatctgggag cccctcgggc cttcaagtac tttgacacca ccatcgaccg
     7021 gaaggtgtac cggagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     7081 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa
     7141 aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat
     7201 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     7261 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7321 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7381 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7441 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     7501 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7561 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7621 tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7681 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7741 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7801 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7861 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7921 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7981 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8341 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8401 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8461 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8521 ggatctcaag aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac
     8581 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8641 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc a
//
