LOCUS       sequence-369027-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABEmax-SpCas9-NGX‐NGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han
            R
  TITLE     Efficient precise in vivo base editing in adult dystrophic mice.
  JOURNAL   Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y.
  PUBMED    34140489
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-17"; volume: "12(1):3719. doi"; pages: " 
            10.1038/s41467-021-23996-y"
COMMENT     Sequence Label: pCMV‐ABEmaxNGX‐NGA
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..43)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(197..785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(277..296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(956..1747,1748..1816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1579..1598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1817..1921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2000..2019)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2191..2570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2571..2774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2725..2745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2816..2835
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2816..2834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2884..2904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4093..8193
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8236..8256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8265..8282
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8305..8322)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8311..8535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8606..8622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8606..8622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8619..8641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8630..8646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8654..8660,8661..8678,8679..8684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8699..8720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
       61 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac
      841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1921 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     1981 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     2041 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     2101 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     2161 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     2221 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2281 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2341 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2401 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2461 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2521 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2581 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2641 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2701 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2761 atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata
     2821 cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag cgagttcgag
     2881 tcaccaaaga agaagcggaa agtctctgaa gtcgagttta gccacgagta ttggatgagg
     2941 cacgcactga ccctggcaaa gcgagcatgg gatgaaagag aagtccccgt gggcgccgtg
     3001 ctggtgcaca acaatagagt gatcggagag ggatggaaca ggccaatcgg ccgccacgac
     3061 cctaccgcac acgcagagat catggcactg aggcagggag gcctggtcat gcagaattac
     3121 cgcctgatcg atgccaccct gtatgtgaca ctggagccat gcgtgatgtg cgcaggagca
     3181 atgatccaca gcaggatcgg aagagtggtg ttcggagcac gggacgccaa gaccggcgca
     3241 gcaggctccc tgatggatgt gctgcaccac cccggcatga accaccgggt ggagatcaca
     3301 gagggaatcc tggcagacga gtgcgccgcc ctgctgagcg atttctttag aatgcggaga
     3361 caggagatca aggcccagaa gaaggcacag agctccaccg actctggagg atctagcgga
     3421 ggatcctctg gaagcgagac accaggcaca agcgagtccg ccacaccaga gagctccggc
     3481 ggctcctccg gaggatcctc tgaggtggag ttttcccacg agtactggat gagacatgcc
     3541 ctgaccctgg ccaagagggc acgcgatgag agggaggtgc ctgtgggagc cgtgctggtg
     3601 ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca cgacccaaca
     3661 gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa ctacagactg
     3721 attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg cgccatgatc
     3781 cactctagga tcggccgcgt ggtgtttggc gtgaggaacg caaaaaccgg cgccgcaggc
     3841 tccctgatgg acgtgctgca ctaccccggc atgaatcacc gcgtcgaaat taccgaggga
     3901 atcctggcag atgaatgtgc cgccctgctg tgctatttct ttcggatgcc tagacaggtg
     3961 ttcaatgctc agaagaaggc ccagagctcc accgactcca gaggatctag cggaggctcc
     4021 tctggctctg agacacctgg cacaagcgag agcgcaacac ctgaaagcag cgggggcagc
     4081 agcggggggt cagacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
     4141 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     4201 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     4261 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     4321 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     4381 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     4441 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     4501 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     4561 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     4621 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     4681 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     4741 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     4801 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     4861 ctggccgagg ataccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     4921 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     4981 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     5041 agcgcctcta tgatcaagct gtacgacgag caccaccagg acctgaccct gctgaaagct
     5101 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     5161 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     5221 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     5281 ctgctgcgga agcagcggac cttcgacaac ggcatcatcc cccaccagat ccacctggga
     5341 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     5401 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     5461 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     5521 ttcgagaagg tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     5581 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     5641 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     5701 gccttcctga gcggcgacca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     5761 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     5821 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     5881 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     5941 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     6001 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     6061 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     6121 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     6181 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     6241 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     6301 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     6361 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     6421 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
     6481 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     6541 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
     6601 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
     6661 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
     6721 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
     6781 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     6841 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     6901 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     6961 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     7021 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     7081 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     7141 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     7201 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     7261 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     7321 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     7381 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc ggcccaagag gaacagcgat
     7441 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgtcagcccc
     7501 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     7561 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     7621 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     7681 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     7741 cgctttctgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     7801 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     7861 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     7921 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     7981 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     8041 ctgggagccc ctcgggcctt caagtacttt gacaccacca tcgaccggaa gcagtaccgg
     8101 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     8161 acacggatcg acctgtctca gctgggaggt gactctggcg gctcaaaaag aaccgccgac
     8221 ggcagcgaat tcgagcccaa gaagaagagg aaagtctaac cggtcatcat caccatcacc
     8281 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     8341 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8401 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8461 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8521 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct
     8581 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8641 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat
     8701 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8761 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt t
//
