LOCUS       sequence-369030-       13027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 13027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST EGFP-flag-CAD
FEATURES             Location/Qualifiers
     source          1..13027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     198..220
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     236..252
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    278..652
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     590..606
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(681..683,684..686,687..1397)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(726..747)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(987..1006)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1334..1355
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8070..8093
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8160..8712
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8304..8321)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8531..8550
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8724..9119
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             9290..9759
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9889..9906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10060..10648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10140..10159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10812..11603,11604..11672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11435..11454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11673..11777)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             11807..12400
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    12463..12820
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             join(12879..13027,1..268)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
       61 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
      121 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
      181 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
      241 tccagccctc actccttctc taggcgcccc catatggcca tatgagatct tatatggggc
      301 acccccgccc cttgtaaact tccctgaccc tgacatgaca agagttacta acagcccctc
      361 tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga gacctctggc
      421 ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc gagtcggcga
      481 cacagtgtgg gtccgccgac accagactaa gaacctagaa cctcgctgga aaggacctta
      541 cacagtcctg ctgaccaccc ccaccgccct caaagtagac ggcatcgcag cttggataca
      601 cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac tgccggatct
      661 agctagttaa ttaaggatcc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      721 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg
      781 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      841 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      901 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      961 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1021 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1081 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1141 tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
     1201 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1261 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     1321 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1381 tggacgagct gtacaaggcg gccctagtgt tggaggacgg gtcggtcctg cggggccagc
     1441 cctttggggc cgccgtgtcg actgccgggg aagtggtgtt tcaaaccggc atggtcggct
     1501 accccgaggc cctcactgat ccctcctaca aggcacagat cttagtgctc acctatcctc
     1561 tgatcggcaa ctatggcatc cccccagatg aaatggatga gttcggtctc tgcaagtggt
     1621 ttgaatcctc gggcatccac gtagcagcac tggtagtggg agagtgctgt cctactccca
     1681 gccactggag tgccacccgc accctgcatg agtggctgca gcagcatggc atccctggct
     1741 tgcaaggagt agacactcgg gagctgacca agaagttgcg ggaacagggg tctctgctgg
     1801 ggaagctggt ccagaatgga acagaacctt caagtctgcc attcttggac cccaatgccc
     1861 gccccctggt accagaggtc tccattaaga ctccacgggt attcaataca gggggtgccc
     1921 ctcggatcct tgctttggac tgtggcctca agtataatca gatccgatgc ctctgccagc
     1981 gtggggctga ggtcactgtg gtaccctggg accatgcact agacagccaa gagtatgagg
     2041 gtctcttctt aagtaatggg cctggtgacc ctgcctccta tcccagtgtc gtatccacac
     2101 tgagccgtgt tttatctgag cctaatcccc gacctgtctt tgggatctgc ctgggacacc
     2161 agctattggc cttagccatt ggggccaaga cttacaagat gagatatggg aaccgaggcc
     2221 ataaccagcc ctgcttgttg gtgggctctg ggcgctgctt tctgacatcc cagaaccatg
     2281 ggtttgctgt ggagacagac tcactgccag cagactgggc tcctctcttc accaacgcca
     2341 atgatggttc caatgaaggc attgtgcaca acagcttgcc tttcttcagt gtccagtttc
     2401 acccagagca ccaagctggc ccttcagata tggaactgct tttcgatatc tttctggaaa
     2461 ctgtgaaaga ggccacagct gggaaccctg ggggccagac agttagagag cggctgactg
     2521 agcgcctctg tccccctggg attcccactc ccggctctgg acttccacca ccacgaaagg
     2581 ttctgatcct gggctcaggg ggcctctcca ttggccaagc tggagaattt gactactcgg
     2641 gctctcaggc aattaaggcc ctgaaggagg aaaacatcca gacgttgctg atcaacccca
     2701 atattgccac agtgcagacc tcccaggggc tggccgacaa ggtctatttt cttcccataa
     2761 cacctcatta tgtaacccag gtgatacgta atgaacgccc cgatggtgtg ttactgactt
     2821 ttgggggcca gactgctctg aactgtggtg tggagctgac caaggccggg gtgctggctc
     2881 ggtatggggt ccgggtcctg ggcacaccag tggagaccat tgagctgacc gaggatcgac
     2941 gggcctttgc tgccagaatg gcagagatcg gagagcatgt ggccccgagc gaggcagcaa
     3001 attctcttga acaggcccag gcagccgctg aacggctggg gtaccctgtg ctagtgcgtg
     3061 cagcctttgc cctgggtggc ctgggctctg gctttgcctc taacagggag gagctctctg
     3121 ctctcgtggc cccagctttt gcccatacca gccaagtgct agtagacaag tctctgaagg
     3181 gatggaagga gattgagtac gaggtggtga gagacgccta tggcaactgt gtcacggtgt
     3241 gtaacatgga gaacttggac ccactgggca tccacactgg tgagtccata gtggtggccc
     3301 ctagccagac actgaatgac agggagtatc agctcctgag gcagacagct atcaaggtga
     3361 cccagcacct gggaattgtt ggggagtgca atgtgcagta tgccttgaac cctgagtctg
     3421 agcagtatta catcattgaa gtgaatgcca ggctctctcg cagctctgcc ctggccagta
     3481 aggccacagg ttatccactg gcttatgtgg cagccaagct agcattgggc atccctttgc
     3541 ctgagctcag gaactctgtg acagggggta cagcagcctt tgaacccagc gtggattatt
     3601 gtgtggtgaa gattcctcga tgggacctta gcaagttcct gcgagtcagc acaaagattg
     3661 ggagctgcat gaagagcgtt ggtgaagtca tgggcattgg gcgttcattt gaggaggcct
     3721 tccagaaggc cctgcgcatg gtggatgaga actgtgtggg ctttgatcac acagtgaaac
     3781 cagtcagcga tatggagttg gagactccaa cagataagcg gatttttgtg gtggcagctg
     3841 ctttgtgggc tggttattca gtggaccgcc tgtatgagct cacacgcatc gaccgctggt
     3901 tcctgcaccg aatgaagcgt atcatcgcac atgcccagct gctagaacaa caccgtggac
     3961 agcctttgcc gccagacctg ctgcaacagg ccaagtgtct tggcttctca gacaaacaga
     4021 ttgcccttgc agttctgagc acagagctgg ctgttcgcaa gctgcgtcag gaactgggga
     4081 tctgtccagc agtgaaacag attgacacag ttgcagctga gtggccagcc cagacaaatt
     4141 acctatacct aacgtattgg ggcaccaccc atgacctcac ctttcgaaca cctcatgtcc
     4201 tagtccttgg ctctggcgtc taccgtattg gctctagcgt tgaatttgac tggtgtgctg
     4261 taggctgcat ccagcagctc cgaaagatgg gatataagac catcatggtg aactataacc
     4321 cagagacagt cagcaccgac tatgacatgt gtgatcgact ctactttgat gagatctctt
     4381 ttgaggtggt gatggacatc tatgagctcg agaaccctga aggtgtgatc ctatccatgg
     4441 gtggacagct gcccaacaac atggccatgg cgttgcatcg gcagcagtgc cgggtgctgg
     4501 gcacctcccc tgaagccatt gactcggctg agaaccgttt caagttttcc cggctccttg
     4561 acaccattgg tatcagccag cctcagtgga gggagctcag tgacctcgag tctgctcgcc
     4621 aattctgcca gaccgtgggg tacccctgtg tggtgcgccc ctcctatgtg ctgagcggtg
     4681 ctgctatgaa tgtggcctac acggatggag acctggagcg cttcctgagc agcgcagcag
     4741 ccgtctccaa agagcatccc gtggtcatct ccaagttcat ccaggaggct aaggagattg
     4801 acgtggatgc cgtggcctct gatggtgtgg tggcagccat cgccatctct gagcatgtgg
     4861 agaatgcagg tgtgcattca ggtgatgcga cgctggtgac ccccccacaa gatatcactg
     4921 ccaaaaccct ggagcggatc aaagccattg tgcatgctgt gggccaggag ctacaggtca
     4981 caggaccctt caatctgcag ctcattgcca aggatgacca gctgaaagtt attgaatgca
     5041 acgtacgtgt ctctcgctcc ttccccttcg tttccaagac actgggtgtg gacctagtag
     5101 ccttggccac gcgggtcatc atgggggaag aagtggaacc tgtggggcta atgactggtt
     5161 ctggagttgt gggagtaaag gtgcctcagt tctccttctc ccgcttggcg ggtgctgacg
     5221 tggtgttggg tgtggaaatg accagtactg gggaggtggc cggctttggg gagagccgct
     5281 gtgaggcata cctcaaggcc atgctaagca ctggctttaa gatccccaag aagaatatcc
     5341 tgctgaccat tggcagctat aagaacaaaa gcgagctgct cccaactgtg cggctactgg
     5401 agagcctggg ctacagcctc tatgccagtc tcggcacagc tgacttctac actgagcatg
     5461 gcgtcaaggt aacagctgtg gactggcact ttgaggaggc tgtggatggt gagtgcccac
     5521 cacagcggag catcctggag cagctagctg agaaaaactt tgagctggtg attaacctgt
     5581 caatgcgtgg agctgggggc cggcgtctct cttcctttgt caccaagggc taccgcaccc
     5641 gacgcttggc cgctgacttc tccgtgcccc taatcatcga tatcaagtgc accaaactct
     5701 ttgtggaggc cctaggccag atcgggccag cccctccttt gaaggtgcat gttgactgta
     5761 tgacctccca aaagcttgtg cgactgccgg gattgattga tgtccatgtg cacctgcggg
     5821 aaccaggtgg gacacataag gaggactttg cttcaggcac agccgctgcc ctggctgggg
     5881 gtatcaccat ggtgtgtgcc atgcctaata cccggccccc catcattgac gcccctgctc
     5941 tggccctggc ccagaagctg gcagaggctg gcgcccggtg cgactttgcg ctattccttg
     6001 gggcctcgtc tgaaaatgca ggaaccttgg gcaccgtggc cgggtctgca gccgggctga
     6061 agctttacct caatgagacc ttctctgagc tgcggctgga cagcgtggtc cagtggatgg
     6121 agcatttcga gacatggccc tcccacctcc ccattgtggc tcacgcggag cagcaaaccg
     6181 tggctgctgt cctcatggtg gctcagctca ctcagcgctc agtgcacata tgtcacgtgg
     6241 cacggaagga ggagatcctg ctaattaaag ctgcaaaggc acggggcttg ccagtgacct
     6301 gcgaggtggc tccccaccac ctgttcctaa gccatgatga cctggagcgc ctggggcctg
     6361 ggaaggggga ggtccggcct gagcttggct cccgccagga tgtggaagcc ctgtgggaga
     6421 acatggctgt catcgactgc tttgcctcag accatgctcc ccataccttg gaggagaagt
     6481 gtgggtccag gcccccacct gggttcccag ggttagagac catgctgcca ctactcctga
     6541 cggctgtaag cgagggccgg ctcagcctgg acgacctgct gcagcgattg caccacaatc
     6601 ctcggcgcat ctttcacctg cctccgcagg aggacaccta tgtggaggtg gatctggagc
     6661 atgagtggac aattcccagc cacatgccct tctccaaggc ccactggaca ccttttgaag
     6721 ggcagaaagt gaagggcacc gtccgccgtg tggtcctgcg aggggaggtt gcctatatcg
     6781 atgggcaggt tctggtaccc ccgggctatg gacaggatgt acggaagtgg ccacaggggg
     6841 ctgttcctca gctcccaccc tcagcccctg ccactagtga gatgaccacg acacctgaaa
     6901 gaccccgccg tggcatccca gggcttcctg atggccgctt ccatctgccg ccccgaatcc
     6961 atcgagcctc cgacccaggt ttgccagctg aggagccaaa ggagaagtcc tctcggaagg
     7021 tagccgagcc agagctgatg ggaacccctg atggcacctg ctaccctcca ccaccagtac
     7081 cgagacaggc atctccccag aacctgggga cccctggctt gctgcacccc cagacctcac
     7141 ccctgctgca ctcattagtg ggccaacata tcctgtccgt ccagcagttc accaaggatc
     7201 agatgtctca cctgttcaat gtggcacaca cactgcgtat gatggtgcag aaggagcgga
     7261 gcctcgacat cctgaagggg aaggtcatgg cctccatgtt ctatgaagtg agcacacgga
     7321 ccagcagctc ctttgcagca gccatggccc ggctgggagg tgctgtgctc agcttctcgg
     7381 aagccacatc gtccgtccag aagggcgaat ccctggctga ctccgtgcag accatgagct
     7441 gctatgccga cgtcgtcgtg ctccggcacc cccagcctgg agcagtggag ctggccgcca
     7501 agcactgccg gaggccagtg atcaatgctg gggatggggt cggagagcac cccacccagg
     7561 ccctgctgga catcttcacc atccgtgagg agctgggaac tgtcaatggc atgacgatca
     7621 cgatggtggg tgacctgaag cacggacgca cagtacattc cctggcctgc ctgctcaccc
     7681 agtatcgtgt cagcctgcgc tacgtggcac ctcccagcct gcgcatgcca cccactgtgc
     7741 gggccttcgt ggcctcccgc ggcaccaagc aggaggaatt cgagagcatt gaggaggcgc
     7801 tgcctgacac tgatgtgctc tacatgactc gaatccagaa ggaacgattt ggctctaccc
     7861 aggagtacga agcttgcttt ggtcagttca tcctcactcc ccacatcatg acccgggcca
     7921 agaagaagat ggtggtgatg cacccgatgc cccgtgtcaa cgagataagc gtggaagtgg
     7981 actcggatcc ccgcgcagcc tacttccgcc aggctgagaa cggcatgtac atccgcatgg
     8041 ctctgttagc caccgtgctg ggccgtttcg actacaaaga cgatgacgac aagtaggaat
     8101 tcctgcaggc ctcgagggcc ggcgcgccgc ggccgctacg taaattcnnn ncccccctaa
     8161 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     8221 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     8281 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     8341 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     8401 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     8461 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     8521 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     8581 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     8641 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     8701 acgatgataa tatggccaca accatggttc ctttgtctca agaagaatcc accctcattg
     8761 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     8821 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     8881 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8941 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     9001 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     9061 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     9121 tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     9181 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     9241 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     9301 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     9361 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     9421 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     9481 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     9541 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     9601 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     9661 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     9721 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     9781 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     9841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    10021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    10081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    10201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    10261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    10381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    10441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    10501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    10561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    10621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    10681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    10741 cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata tgagtaaact
    10801 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10861 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10921 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10981 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11041 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11101 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11161 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11221 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11281 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11341 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11401 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11461 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11521 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11581 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11641 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11701 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11761 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcataatg aaagacccca
    11821 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
    11881 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
    11941 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
    12001 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
    12061 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
    12121 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
    12181 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
    12241 aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
    12301 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
    12361 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
    12421 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
    12481 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
    12541 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
    12601 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
    12661 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc cccctaatag gagggatatg
    12721 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
    12781 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
    12841 tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct
    12901 taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag
    12961 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg
    13021 gatggcc
//
