LOCUS       sequence-369031-       12327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged human CAD T1348A in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD T1348A
FEATURES             Location/Qualifiers
     source          1..12327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..52
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    78..452
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     390..406
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      479..488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7156..7179
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7260..7812
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7404..7421)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7631..7650
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7824..8219
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8390..8859
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8989..9006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9160..9748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9240..9259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9912..10703,10704..10772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10535..10554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10773..10877)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             10907..11500
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    11563..11920
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             join(11979..12327,1..68)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     join(12325..12327,1..20)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
       61 taggcgcccc catatggcca tatgagatct tatatggggc acccccgccc cttgtaaact
      121 tccctgaccc tgacatgaca agagttacta acagcccctc tctccaagct cacttacagg
      181 ctctctactt agtccagcac gaagtctgga gacctctggc ggcagcctac caagaacaac
      241 tggaccgacc ggtggtacct cacccttacc gagtcggcga cacagtgtgg gtccgccgac
      301 accagactaa gaacctagaa cctcgctgga aaggacctta cacagtcctg ctgaccaccc
      361 ccaccgccct caaagtagac ggcatcgcag cttggataca cgccgcccac gtgaaggctg
      421 ccgaccccgg gggtggacca tcctctagac tgccggatct agctagttaa ttaaggatcc
      481 atggcggccc tagtgttgga ggacgggtcg gtcctgcggg gccagccctt tggggccgcc
      541 gtgtcgactg ccggggaagt ggtgtttcaa accggcatgg tcggctaccc cgaggccctc
      601 actgatccct cctacaaggc acagatctta gtgctcacct atcctctgat cggcaactat
      661 ggcatccccc cagatgaaat ggatgagttc ggtctctgca agtggtttga atcctcgggc
      721 atccacgtag cagcactggt agtgggagag tgctgtccta ctcccagcca ctggagtgcc
      781 acccgcaccc tgcatgagtg gctgcagcag catggcatcc ctggcttgca aggagtagac
      841 actcgggagc tgaccaagaa gttgcgggaa caggggtctc tgctggggaa gctggtccag
      901 aatggaacag aaccttcaag tctgccattc ttggacccca atgcccgccc cctggtacca
      961 gaggtctcca ttaagactcc acgggtattc aatacagggg gtgcccctcg gatccttgct
     1021 ttggactgtg gcctcaagta taatcagatc cgatgcctct gccagcgtgg ggctgaggtc
     1081 actgtggtac cctgggacca tgcactagac agccaagagt atgagggtct cttcttaagt
     1141 aatgggcctg gtgaccctgc ctcctatccc agtgtcgtat ccacactgag ccgtgtttta
     1201 tctgagccta atccccgacc tgtctttggg atctgcctgg gacaccagct attggcctta
     1261 gccattgggg ccaagactta caagatgaga tatgggaacc gaggccataa ccagccctgc
     1321 ttgttggtgg gctctgggcg ctgctttctg acatcccaga accatgggtt tgctgtggag
     1381 acagactcac tgccagcaga ctgggctcct ctcttcacca acgccaatga tggttccaat
     1441 gaaggcattg tgcacaacag cttgcctttc ttcagtgtcc agtttcaccc agagcaccaa
     1501 gctggccctt cagatatgga actgcttttc gatatctttc tggaaactgt gaaagaggcc
     1561 acagctggga accctggggg ccagacagtt agagagcggc tgactgagcg cctctgtccc
     1621 cctgggattc ccactcccgg ctctggactt ccaccaccac gaaaggttct gatcctgggc
     1681 tcagggggcc tctccattgg ccaagctgga gaatttgact actcgggctc tcaggcaatt
     1741 aaggccctga aggaggaaaa catccagacg ttgctgatca accccaatat tgccacagtg
     1801 cagacctccc aggggctggc cgacaaggtc tattttcttc ccataacacc tcattatgta
     1861 acccaggtga tacgtaatga acgccccgat ggtgtgttac tgacttttgg gggccagact
     1921 gctctgaact gtggtgtgga gctgaccaag gccggggtgc tggctcggta tggggtccgg
     1981 gtcctgggca caccagtgga gaccattgag ctgaccgagg atcgacgggc ctttgctgcc
     2041 agaatggcag agatcggaga gcatgtggcc ccgagcgagg cagcaaattc tcttgaacag
     2101 gcccaggcag ccgctgaacg gctggggtac cctgtgctag tgcgtgcagc ctttgccctg
     2161 ggtggcctgg gctctggctt tgcctctaac agggaggagc tctctgctct cgtggcccca
     2221 gcttttgccc ataccagcca agtgctagta gacaagtctc tgaagggatg gaaggagatt
     2281 gagtacgagg tggtgagaga cgcctatggc aactgtgtca cggtgtgtaa catggagaac
     2341 ttggacccac tgggcatcca cactggtgag tccatagtgg tggcccctag ccagacactg
     2401 aatgacaggg agtatcagct cctgaggcag acagctatca aggtgaccca gcacctggga
     2461 attgttgggg agtgcaatgt gcagtatgcc ttgaaccctg agtctgagca gtattacatc
     2521 attgaagtga atgccaggct ctctcgcagc tctgccctgg ccagtaaggc cacaggttat
     2581 ccactggctt atgtggcagc caagctagca ttgggcatcc ctttgcctga gctcaggaac
     2641 tctgtgacag ggggtacagc agcctttgaa cccagcgtgg attattgtgt ggtgaagatt
     2701 cctcgatggg accttagcaa gttcctgcga gtcagcacaa agattgggag ctgcatgaag
     2761 agcgttggtg aagtcatggg cattgggcgt tcatttgagg aggccttcca gaaggccctg
     2821 cgcatggtgg atgagaactg tgtgggcttt gatcacacag tgaaaccagt cagcgatatg
     2881 gagttggaga ctccaacaga taagcggatt tttgtggtgg cagctgcttt gtgggctggt
     2941 tattcagtgg accgcctgta tgagctcaca cgcatcgacc gctggttcct gcaccgaatg
     3001 aagcgtatca tcgcacatgc ccagctgcta gaacaacacc gtggacagcc tttgccgcca
     3061 gacctgctgc aacaggccaa gtgtcttggc ttctcagaca aacagattgc ccttgcagtt
     3121 ctgagcacag agctggctgt tcgcaagctg cgtcaggaac tggggatctg tccagcagtg
     3181 aaacagattg acacagttgc agctgagtgg ccagcccaga caaattacct atacctaacg
     3241 tattggggca ccacccatga cctcaccttt cgaacacctc atgtcctagt ccttggctct
     3301 ggcgtctacc gtattggctc tagcgttgaa tttgactggt gtgctgtagg ctgcatccag
     3361 cagctccgaa agatgggata taagaccatc atggtgaact ataacccaga gacagtcagc
     3421 accgactatg acatgtgtga tcgactctac tttgatgaga tctcttttga ggtggtgatg
     3481 gacatctatg agctcgagaa ccctgaaggt gtgatcctat ccatgggtgg acagctgccc
     3541 aacaacatgg ccatggcgtt gcatcggcag cagtgccggg tgctgggcac ctcccctgaa
     3601 gccattgact cggctgagaa ccgtttcaag ttttcccggc tccttgacac cattggtatc
     3661 agccagcctc agtggaggga gctcagtgac ctcgagtctg ctcgccaatt ctgccagacc
     3721 gtggggtacc cctgtgtggt gcgcccctcc tatgtgctga gcggtgctgc tatgaatgtg
     3781 gcctacacgg atggagacct ggagcgcttc ctgagcagcg cagcagccgt ctccaaagag
     3841 catcccgtgg tcatctccaa gttcatccag gaggctaagg agattgacgt ggatgccgtg
     3901 gcctctgatg gtgtggtggc agccatcgcc atctctgagc atgtggagaa tgcaggtgtg
     3961 cattcaggtg atgcgacgct ggtgaccccc ccacaagata tcactgccaa aaccctggag
     4021 cggatcaaag ccattgtgca tgctgtgggc caggagctac aggtcacagg acccttcaat
     4081 ctgcagctca ttgccaagga tgaccagctg aaagttattg aatgcaacgt acgtgtctct
     4141 cgctccttcc ccttcgtttc caagacactg ggtgtggacc tagtagcctt ggccacgcgg
     4201 gtcatcatgg gggaagaagt ggaacctgtg gggctaatga ctggttctgg agttgtggga
     4261 gtaaaggtgc ctcagttctc cttctcccgc ttggcgggtg ctgacgtggt gttgggtgtg
     4321 gaaatgacca gtactgggga ggtggccggc tttggggaga gccgctgtga ggcatacctc
     4381 aaggccatgc taagcactgg ctttaagatc cccaagaaga atatcctgct gaccattggc
     4441 agctataaga acaaaagcga gctgctccca actgtgcggc tactggagag cctgggctac
     4501 agcctctatg ccagtctcgg cgcagctgac ttctacactg agcatggcgt caaggtaaca
     4561 gctgtggact ggcactttga ggaggctgtg gatggtgagt gcccaccaca gcggagcatc
     4621 ctggagcagc tagctgagaa aaactttgag ctggtgatta acctgtcaat gcgtggagct
     4681 gggggccggc gtctctcttc ctttgtcacc aagggctacc gcacccgacg cttggccgct
     4741 gacttctccg tgcccctaat catcgatatc aagtgcacca aactctttgt ggaggcccta
     4801 ggccagatcg ggccagcccc tcctttgaag gtgcatgttg actgtatgac ctcccaaaag
     4861 cttgtgcgac tgccgggatt gattgatgtc catgtgcacc tgcgggaacc aggtgggaca
     4921 cataaggagg actttgcttc aggcacagcc gctgccctgg ctgggggtat caccatggtg
     4981 tgtgccatgc ctaatacccg gccccccatc attgacgccc ctgctctggc cctggcccag
     5041 aagctggcag aggctggcgc ccggtgcgac tttgcgctat tccttggggc ctcgtctgaa
     5101 aatgcaggaa ccttgggcac cgtggccggg tctgcagccg ggctgaagct ttacctcaat
     5161 gagaccttct ctgagctgcg gctggacagc gtggtccagt ggatggagca tttcgagaca
     5221 tggccctccc acctccccat tgtggctcac gcggagcagc aaaccgtggc tgctgtcctc
     5281 atggtggctc agctcactca gcgctcagtg cacatatgtc acgtggcacg gaaggaggag
     5341 atcctgctaa ttaaagctgc aaaggcacgg ggcttgccag tgacctgcga ggtggctccc
     5401 caccacctgt tcctaagcca tgatgacctg gagcgcctgg ggcctgggaa gggggaggtc
     5461 cggcctgagc ttggctcccg ccaggatgtg gaagccctgt gggagaacat ggctgtcatc
     5521 gactgctttg cctcagacca tgctccccat accttggagg agaagtgtgg gtccaggccc
     5581 ccacctgggt tcccagggtt agagaccatg ctgccactac tcctgacggc tgtaagcgag
     5641 ggccggctca gcctggacga cctgctgcag cgattgcacc acaatcctcg gcgcatcttt
     5701 cacctgcctc cgcaggagga cacctatgtg gaggtggatc tggagcatga gtggacaatt
     5761 cccagccaca tgcccttctc caaggcccac tggacacctt ttgaagggca gaaagtgaag
     5821 ggcaccgtcc gccgtgtggt cctgcgaggg gaggttgcct atatcgatgg gcaggttctg
     5881 gtacccccgg gctatggaca ggatgtacgg aagtggccac agggggctgt tcctcagctc
     5941 ccaccctcag cccctgccac tagtgagatg accacgacac ctgaaagacc ccgccgtggc
     6001 atcccagggc ttcctgatgg ccgcttccat ctgccgcccc gaatccatcg agcctccgac
     6061 ccaggtttgc cagctgagga gccaaaggag aagtcctctc ggaaggtagc cgagccagag
     6121 ctgatgggaa cccctgatgg cacctgctac cctccaccac cagtaccgag acaggcatct
     6181 ccccagaacc tggggacccc tggcttgctg cacccccaga cctcacccct gctgcactca
     6241 ttagtgggcc aacatatcct gtccgtccag cagttcacca aggatcagat gtctcacctg
     6301 ttcaatgtgg cacacacact gcgtatgatg gtgcagaagg agcggagcct cgacatcctg
     6361 aaggggaagg tcatggcctc catgttctat gaagtgagca cacggaccag cagctccttt
     6421 gcagcagcca tggcccggct gggaggtgct gtgctcagct tctcggaagc cacatcgtcc
     6481 gtccagaagg gcgaatccct ggctgactcc gtgcagacca tgagctgcta tgccgacgtc
     6541 gtcgtgctcc ggcaccccca gcctggagca gtggagctgg ccgccaagca ctgccggagg
     6601 ccagtgatca atgctgggga tggggtcgga gagcacccca cccaggccct gctggacatc
     6661 ttcaccatcc gtgaggagct gggaactgtc aatggcatga cgatcacgat ggtgggtgac
     6721 ctgaagcacg gacgcacagt acattccctg gcctgcctgc tcacccagta tcgtgtcagc
     6781 ctgcgctacg tggcacctcc cagcctgcgc atgccaccca ctgtgcgggc cttcgtggcc
     6841 tcccgcggca ccaagcagga ggaattcgag agcattgagg aggcgctgcc tgacactgat
     6901 gtgctctaca tgactcgaat ccagaaggaa cgatttggct ctacccagga gtacgaagct
     6961 tgctttggtc agttcatcct cactccccac atcatgaccc gggccaagaa gaagatggtg
     7021 gtgatgcacc cgatgccccg tgtcaacgag ataagcgtgg aagtggactc ggatccccgc
     7081 gcagcctact tccgccaggc tgagaacggc atgtacatcc gcatggctct gttagccacc
     7141 gtgctgggcc gtttcgacta caaagacgat gacgacaagt aggaattcct gcaggcctcg
     7201 agggccggcg cgccgcggcc gctacgtaaa ttccgccccc ccccccyccc ccccccctaa
     7261 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     7321 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     7381 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     7441 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     7501 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     7561 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     7621 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     7681 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     7741 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     7801 acgatgataa tatggccaca accatggttc ctttgtctca agaagaatcc accctcattg
     7861 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     7921 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     7981 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8041 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     8101 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     8161 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     8221 tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     8281 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     8341 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     8401 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     8461 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     8521 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     8581 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     8641 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     8701 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     8761 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     8821 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     8881 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     8941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9841 cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata tgagtaaact
     9901 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9961 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10021 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10081 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10141 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10201 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10261 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10321 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10381 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10441 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10501 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10561 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10621 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10681 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10741 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10801 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10861 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcataatg aaagacccca
    10921 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
    10981 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
    11041 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
    11101 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
    11161 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
    11221 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
    11281 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
    11341 aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
    11401 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
    11461 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
    11521 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
    11581 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
    11641 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
    11701 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
    11761 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc cccctaatag gagggatatg
    11821 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
    11881 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
    11941 tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct
    12001 taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag
    12061 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg
    12121 gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct
    12181 tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct
    12241 tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc
    12301 ttcctccatc cgccccgtct ctccccc
//
