LOCUS       sequence-369032-        3117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  provides sulfadiazine resistance (sulI) as a level 0 GoldenBraid 
            part (CDS; B3-B4-B5).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Kobercova E, Srba M, Fischer L
  TITLE     Sulfadiazine and phosphinothricin selection systems optimised for 
            the transformation of tobacco BY-2 cells.
  JOURNAL   Plant Cell Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7.
  PUBMED    36609768
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7."
COMMENT     Sequence Label: pUPD2_SulR(CDS)
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      38..95
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(119..136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(290..878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(370..389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1061..1155)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1176..1835)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1750..1769
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1997..2015
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2019..2062)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2065..2086
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt
       61 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct
      121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
      301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1021 gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg
     1081 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1141 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcgtcg
     1201 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1261 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1321 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1381 ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag
     1441 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1501 tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1561 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1621 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1681 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1741 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1801 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1861 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1921 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     1981 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     2041 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggtctc
     2101 gaatggcatc tatgatttct tcttcagctg tgactacagt cagccgtgct tctacggtgc
     2161 aatcggccgc tgtggctcca ttcggcggcc tcaaatccat gactggattc ccagttaaga
     2221 aggtcaacac tgacattact tccattacaa gcaatggtgg aagagtaaag tgcatggtga
     2281 cggtgttcgg cattctgaat ctcaccgagg actccttctt cgatgagagc cggcggctag
     2341 accccgccgg cgctgtcacg gctgcgatcg aaatgctgcg agtcggatca gacgtcgtgg
     2401 atgtcggacc ggccgccagc catccggacg cgaggccagt atcgccggcc gatgagatca
     2461 gacgtattgc gccgctctta gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca
     2521 gcttccaacc ggaaacccag cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgaca
     2581 tccaaggatt tcctgaccct gcgctctatc ccgatattgc tgaggcggac tgtaggctgg
     2641 tggttatgca ctcagcgcag cgggatggca tcgccacccg caccggtcac cttcgacccg
     2701 aagacgcgct cgacgagatt gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga
     2761 gcggggtcgc tgccgaccgg ctcatcctcg atccggggat gggatttttc ttgagccccg
     2821 caccggaaac atcgctgcac gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc
     2881 ttccgctatt ggtatcggtg tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg
     2941 taaaggatct gggtccagcg agccttgcgg cggaacttca cgcgatcggc aatggcgctg
     3001 actacgtccg cacccacgcg cctggagatc tgcgaagcgc aatcaccatc tcggaaaccc
     3061 tcgcgaaatt tcgcagtcgc gacgccagag atcgagggtt agatcatgcc taagctt
//
