LOCUS       sequence-36904-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4179 (=HL 865 + pHL 1381)
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(64..81)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      234..320
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      412..439
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    532..548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atggatgagc tctacaaata aaagctagct tggctgtttt ggcggatgag agaagatttt
       61 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
      121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
      181 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
      241 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      301 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      361 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
      421 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat
      481 tcaaatatgt atccgctcag gatccataaa tgtgagcgga taacattgac attgtgagcg
      541 gataacaaga tactcccggg taaggaggaa aaaaaatggc taaggggcaa tctttacaag
      601 atccgttcct gaacgcactg cgtcgggaac gtgttccagt ttctatttat ttggtgaatg
      661 gtattaagct gcaagggcaa atcgagtctt ttgatcagtt cgtgatcctg ttgaaaaaca
      721 cggtcagcca gatggtttac aagcacgcga t
//
