LOCUS       sequence-369042-       11791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along 
            with synaptophysin-HaloTag in neuronal cells, separated by a P2A 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Bradberry MM, Chapman ER
  TITLE     All-optical monitoring of excitation-secretion coupling demonstrates
            that SV2A functions downstream of evoked Ca(2+) entry.
  JOURNAL   J Physiol. 2022 Feb;600(3):645-654. doi: 10.1113/JP282601. Epub 2022
            Jan 13.
  PUBMED    34957569
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1113/JP282601"; 
            journalName: "J Physiol"; date: "2022-02"; volume: "600"; issue: 
            "3"; pages: "645-654"
COMMENT     Sequence Label: pFSW GAP43-jRGECO1a P2A Syp-Halo
FEATURES             Location/Qualifiers
     source          1..11791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3137..3154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3158..3190
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(3194..3202,3203..3217)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             3245..3301
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     3997..4016
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4490..4546
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5513..5533
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             5549..6430
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    6483..7071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6536..6556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6954..6965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7074..7090)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7075..7091)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7596..7776
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7802..7819)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7808..8032
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8078..8506
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8165..8184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8375..8396
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8515..8535)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8520..8849
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8700..8835
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8762..8781
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8897..8944
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8963..9337
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9467..9588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9504..9523)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9558..9577
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9637..9653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9637..9653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9650..9672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9661..9677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9685..9691,9692..9709,9710..9715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9730..9751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9868..9885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10039..10627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10119..10138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10798..11589,11590..11658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11421..11440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11659..11763)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatcgccacc atgctgtgct gtatgagaag aaccaaacag gttgaaaaga atgatgagga
     3121 ccaaaagatt ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
     3181 gcaaatgggt cgggatctgt acgacgatga cgataaggat ctcgcaacaa tggtcgactc
     3241 atcacgtcgt aagtggaata aggcaggtca cgcagtcaga gctataggtc ggctgagctc
     3301 acccgtggtt tccgagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa
     3361 ggggctgagg ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc
     3421 caagaagccc gtgcagctgc ccggcgccta catcgtggac atcaagttgg acatcgtgtc
     3481 ccacaacgag gactacacca tcgtggaaca gtgcgaacgc gccgagggcc gccactccac
     3541 cggcggcatg gacgagctgt acaagggagg tacaggcggg agtctggtga gcaagggcga
     3601 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3661 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt
     3721 tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3781 gtcccctcag ttcatgtacg gctccaaggc ctacattaag cacccagccg acatccccga
     3841 ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga
     3901 cggcggcatt attcacgtga accaggactc ctccctgcag gacggcgtat tcatctacaa
     3961 ggtgaagctg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     4021 gggctgggag gctacgcgtg acgacctgac tgaagagcag atcgcagaat ttaaagaggc
     4081 tttctcccta tttgacaagg acggggatgg gacgataaca accaaggagc ttgggacggt
     4141 gttccgatcg ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt
     4201 agatgccgac ggtgacggca cattcgactt ccctgagttc ctgacgatga tggcaagaaa
     4261 aatgaatgac acagacagtg aagaggaaat tcgcgaagcg ttccgcgtgt ttgataagga
     4321 cggcaatggc tacatcggcg cagcagagct tcgccacgtg atgacagacc ttggagagaa
     4381 gttaacagat gaggaggttg atgaaatgat cagggtagca gacatcgatg gggatggtca
     4441 ggtaaactac gaagagtttg tacaaatgat gacagcgaag ggaagcggag ctactaactt
     4501 cagcctgctg aagcaagctg gagacgtgga ggagaaccct ggacctatgg acgtggtgaa
     4561 tcagctggtg gctgggggtc agttccgggt ggtcaaggag ccccttggct tcgtgaaggt
     4621 gctgcagtgg gtctttgcca tcttcgcctt tgctacgtgt ggcagctaca ccggggagct
     4681 tcggctgagc gtggagtgtg ccaacaagac ggagagtgcc ctcaacatcg aagttgaatt
     4741 cgagtacccc ttcaggctgc accaagtgta ctttgatgca ccctcctgcg tcaaaggggg
     4801 cactaccaag atcttcctgg ttggggacta ctcctcgtcg gctgaattct ttgtcaccgt
     4861 ggctgtgttt gccttcctct actccatggg ggccctggcc acctacatct tcctgcagaa
     4921 caagtaccga gagaacaaca aagggcctat gatggacttt ctggctacag ccgtgttcgc
     4981 tttcatgtgg ctagttagtt catcagcctg ggccaaaggc ctgtccgatg tgaagatggc
     5041 cacggaccca gagaacatta tcaaggagat gcccatgtgc cgccagacag ggaacacatg
     5101 caaggaactg agggaccctg tgacttcagg actcaacacc tcagtggtgt ttggcttcct
     5161 gaacctggtg ctctgggttg gcaacttatg gttcgtgttc aaggagacag gctgggcagc
     5221 cccattcatg cgcgcacctc caggcgcccc ggaaaagcaa ccagcacctg gcgatgccta
     5281 cggcgatgcg ggctacgggc agggccccgg aggctatggg ccccaggact cctacgggcc
     5341 tcagggtggt tatcaacccg attacgggca gccagccagc ggtggcggtg gctacgggcc
     5401 tcagggcgac tatgggcagc aaggctatgg ccaacagggt gcgcccacct ccttctccaa
     5461 tcagatgaaa ggatccggaa gttcggggtc cagcggttct tcaggcagtt ccgaggatct
     5521 gtactttcag agcgataacg atggatccga aatcggtact ggctttccat tcgaccccca
     5581 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
     5641 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
     5701 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
     5761 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
     5821 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
     5881 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
     5941 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
     6001 caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct
     6061 gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt
     6121 cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg
     6181 tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc
     6241 tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc
     6301 tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct
     6361 gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtctactct
     6421 ggagatttcc tgaggccgca ctcctcaggt gcaggctgca attcgatatc aagcttatcg
     6481 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6841 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7081 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7141 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7201 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7261 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7321 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     7381 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     7441 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     7501 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     7561 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     7621 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7681 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7741 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     7801 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7861 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7921 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7981 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8041 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8101 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8161 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8221 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8281 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8341 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8401 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8461 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     8521 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8581 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8641 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8701 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8761 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8821 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8881 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8941 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     9001 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     9061 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     9121 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     9181 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     9241 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     9301 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     9361 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9421 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     9481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9601 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9841 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9901 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9961 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10021 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10081 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10141 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10201 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10261 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10321 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10381 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10441 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10501 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11761 gcgcacattt ccccgaaaag tgccacctga c
//
