LOCUS       sequence-369044-        8510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite destination vector for ascidian electroporation of an 
            AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined 
            reg. sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
FEATURES             Location/Qualifiers
     source          1..8510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..156
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(585..890)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(687..706)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1210..1893)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     1808..1827
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1894..1996)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2174..2297)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2325..2341)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2325..2341)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    2371..2395
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2429..2449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2537..5584
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2539..2561)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(5609..5633)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5971..6105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6008..6027)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6062..6081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6141..6160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6142..6160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6247..6264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6418..7006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6498..6517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7177..7968,7969..8037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7800..7819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8038..8142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8210..8228
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8266..8288)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8388..8407
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8488..8506
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8488..8505
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     join(8509..8510,1..15)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     join(8510,1..16)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaggtcgacg gtatcgataa gcttgatggg gacaagtttg tataataaag ttgaacgaga
       61 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      121 ataatactgt aaaacacaac atatccagtc actatgaatc aactacttag atggtattag
      181 tgacctgtag tcgaccgaca gccttccaaa tgttcttcgg gtgatgctgc caacttagtc
      241 gaccgacagc cttccaaatg ttcttctcaa acggaatcgt cgtatccagc ctactcgcta
      301 ttgtcctcaa tgccgtatta aatcataaaa agaaataaga aaaagaggtg cgagcctctt
      361 ttttgtgtga caaaataaaa acatctacct attcatatac gctagtgtca tagtcctgaa
      421 aatcatctgc atcaagaaca atttcacaac tcttatactt ttctcttaca agtcgttcgg
      481 cttcatctgg attttcagcc tctatactta ctaaacgtga taaagtttct gtaatttcta
      541 ctgtatcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
      601 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
      661 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
      721 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
      781 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
      841 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
      901 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
      961 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     1021 tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg
     1081 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
     1141 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
     1201 atctggcttt tagtaagccg gatccacgcg gcgtttacgc cccccctgcc actcatcgca
     1261 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1321 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1381 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1441 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     1501 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1561 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1621 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
     1681 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1741 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1801 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1861 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1921 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1981 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc
     2041 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
     2101 ggcgcaaagt gcgtcgggtg atgctgccaa cttagtcgac tacaggtcac taataccatc
     2161 taagtagttg attcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     2221 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac
     2281 ttttgtatac aaagtggtcc ccatcgaatt cctgcagccc gggggatcca ctagttctag
     2341 aggatccatt taaataggcc tttgtttatc acaagtttgt acaaaaaagc aggctgcaag
     2401 ggtaccgagc tcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac cggtgggtga
     2461 agaccagaaa cagcacctcg aactgagccg cgatattgcc cagcgtttca acgcgctgta
     2521 tggcgagatc gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2581 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2641 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
     2701 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
     2761 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
     2821 gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
     2881 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
     2941 tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca
     3001 ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg
     3061 cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg
     3121 gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag
     3181 cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga
     3241 agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg
     3301 tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg
     3361 tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc
     3421 cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat
     3481 tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct
     3541 gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca
     3601 tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa
     3661 ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga
     3721 ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat
     3781 gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat
     3841 ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg
     3901 ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc
     3961 ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta
     4021 cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg
     4081 gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg
     4141 taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca
     4201 gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa
     4261 cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat
     4321 gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca
     4381 gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct
     4441 gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct
     4501 ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc
     4561 tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc
     4621 gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc
     4681 ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag
     4741 cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg
     4801 ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca
     4861 gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc
     4921 taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt
     4981 gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca
     5041 gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca
     5101 aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg
     5161 cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca
     5221 agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc
     5281 agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga
     5341 attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag
     5401 tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg
     5461 gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt
     5521 atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa
     5581 ataataataa ccgggcaggc catgtctgac ccagctttct tgtacaaagt ggtgataaac
     5641 ttagatatcg gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca
     5701 aaaataataa taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat
     5761 gtactattta aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt
     5821 tatcatggga gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag
     5881 caaaagtgat acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc
     5941 gctgtttggt ctgctttctg acaaactcgg aacttgttta ttgcagctta taatggttac
     6001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgacaa agtcaaagcg
     6121 gccatcagat ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa
     6181 cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6241 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6301 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6361 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6421 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6481 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6541 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6601 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6661 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6721 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6781 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6841 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6901 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6961 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7021 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7081 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7141 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7201 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7261 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7321 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7381 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7441 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7501 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7561 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7621 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7681 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7741 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7801 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7861 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7921 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7981 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8041 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8161 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8221 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     8281 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     8341 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     8401 gattgtactg agagtgcacc atatggacat attgtcgtta gaacgcggct acaattaata
     8461 cataacctta tgtatcatac acatacgatt taggtgacac tatagaactc
//
